No abstract

In the bacterial enzyme-induced calcite precipitation (BEICP) technique for biocementation, the spatial distribution of adsorbed and catalytically active urease dictates the location where calcium carbonate precipitation and resulting cementation will occur. This study investigated the relationships between the amount of urease and total bacterial proteins adsorbed, the retained enzymatic activity of adsorbed urease, and the overall loss of activity upon adsorption, and how these relationships are influenced by changes in soil surface chemistry. In soils with hydrophobic contents higher than 20% (w/w) ratio, urease was preferentially adsorbed compared to the total amount of proteins present in the crude bacterial protein extract. Conversely, adsorption of urease onto silica sand and soil mixtures, including iron-coated sand, was much lower compared to the total proteins. Higher levels of urease activity were retained in hydrophobic-containing samples, with urease activity decreasing with lower hydrophobic content. These observations suggest that the surface manipulation of soils, such as treatments to add hydrophobicity to soil surfaces, can potentially be used to increase the activity of adsorbed urease to improve biocementation outcomes.