Adsorption of bile acid by chitosan salts prepared with cinnamic acid and analogue compounds

Abstract

A chitosan (CS) powder treated with cinnamic acid and an analogue compound (CN) was prepared as CS-CN. Using it, bile acid adsorption by CS-CN and the release of CN were investigated in vitro. When CS-CN was soaked in a taurocholate solution, it released CN and simultaneously adsorbed the bile acid. For CS-CN prepared with cinnamic acid, the amount of CN released was 0.286 +/- 0.001 mmol/g CS-CN; the amount of taurocholate adsorbed was 0.284 +/- 0.003 mmol/g CS-CN. These two functions were recognized on alginate or pectin gel beads containing CS-CN. The amount of released CN was altered extensively by the species of CN used for gel-bead preparation. Results suggest that CS-CN is a candidate for complementary medicine to prevent lifestyle-related diseases.

Dietary chitosan enhances hepatic CYP7A1 activity and reduces plasma and liver cholesterol concentrations in diet-induced hypercholesterolemia in rats

“…Chitosan has been shown to be hypocholesterolemic based on the mechanism of greater excretion of bile acids and total steroids leading to an up-regulation of bile acid biosynthesis (Fukada et al, 1991; Gallaher et al, 2000; Maezaki et al, 1993; Murata et al, 2006). However, the effects of chitosan on the bile acid synthesizing enzyme, CYP7A1, have not been investigated.…”

Uptake of Bile Acid into Calcium-Induced Alginate Gel Beads Containing <i>β</i>-Chitosan Weak Acid Salt

“…One mechanism by which this occurs is via adsorption of bile acids within the intestinal tract. We have previously reported that a weak acid salt of α-CS adsorbs bile acids through electrostatic interactions and that oral administration of the salt reduces serum cholesterol levels in rats [10] [11]. We have also shown that a polymer matrix containing α-CS salt adsorbs bile acids by the same mechanism [12].…”

Functions of Chitosan-Ferulic Acid Salt for Prevention of Hypertension

“…In a mouse study, the low bile acid-binding capabilities of CS have been shown (Bennekum et al, 2005). We reported that CS salts adsorb BA in vitro, but this phenomenon has not been observed in the case of CS itself (Murata et al, 2006). Therefore, following oral administration of CS, the cationic polysaccharide might turn into HCl salt in the human stomach and subsequently bind BA through an ion-exchange reaction.…”