Adsorption of a styrene maleic acid (SMA) copolymer-stabilized phospholipid nanodisc on a solid-supported planar lipid bilayer

Hall, Stephen B.; Clifton, Luke A.; Tognoloni, Cecilia; Morrison, Kerrie A.; Knowles, Timothy J.; Kinane, C. J.; Dafforn, Timothy R.; Edler, Karen J.; Arnold, Thomas

doi:10.1016/j.jcis.2020.04.013

Cited by 11 publications

(20 citation statements)

References 52 publications

(67 reference statements)

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“…Such monolayer surfaces are useful for surface pressure isotherms of embedded MPs and techniques apt to probe the protein orientation and surface coverage of reconstituted proteins, such as X-ray and neutron reflectivity. [2,6] Limitations of monolayers not simulating the double leaflet bilayer property of membranes explains the heightened relative popularity of SLBs, which are extended planar lipid bilayers often generated by Langmuir-Blodgett transfer or liposome (lipid vesicle) fusion onto a SiO 2 or modified gold surface. [7] SLBs are undoubtedly suitable for reconstituting certain peripheral and integral membrane proteins (which protrude through one membrane side) for use in surface sensitive measurement approaches including quartz crystal microbalance with dissipation monitoring [8] and total internal reflection fluorescence [6,9] of protein association properties.…”

Section: Membrane Mimetic Technology: a Brief Historymentioning

confidence: 99%

“…[2,6] Limitations of monolayers not simulating the double leaflet bilayer property of membranes explains the heightened relative popularity of SLBs, which are extended planar lipid bilayers often generated by Langmuir-Blodgett transfer or liposome (lipid vesicle) fusion onto a SiO 2 or modified gold surface. [7] SLBs are undoubtedly suitable for reconstituting certain peripheral and integral membrane proteins (which protrude through one membrane side) for use in surface sensitive measurement approaches including quartz crystal microbalance with dissipation monitoring [8] and total internal reflection fluorescence [6,9] of protein association properties. However, integral membrane proteins take up space on both intra-and extracellular sides of the lipid bilayer sheet to varying degrees, a property which is often better accommodated by tethered lipid bilayers making use of macromolecules such as polymer cushions.…”

Section: Membrane Mimetic Technology: a Brief Historymentioning

confidence: 99%

“…Furthermore, SMI is less efficient at MP solubilisation from biological membranes than SMA. [6] SMAd-A and SMI are able to solubilise MPs into nanodiscs below a neutral pH of~7-7.5 whilst displaying remarkable tolerance to divalent metal ions. [65][66] SMA-QA contains an ammonium group which is consistently charged independent of pH value, allowing for a broad applicability across pH and buffer conditions.…”

Section: Functionally Modified Smanh Derivatives: Smadsmentioning

confidence: 99%

“… [2,3,5] Lipid monolayer films consist of a 2D planar sheet of lipids in a single leaflet extended across an amphipathic interface, being either the solid–water (such as upon SiO 2 ) or the air–water interface. Such monolayer surfaces are useful for surface pressure isotherms of embedded MPs and techniques apt to probe the protein orientation and surface coverage of reconstituted proteins, such as X‐ray and neutron reflectivity [2,6] . Limitations of monolayers not simulating the double leaflet bilayer property of membranes explains the heightened relative popularity of SLBs, which are extended planar lipid bilayers often generated by Langmuir–Blodgett transfer or liposome (lipid vesicle) fusion onto a SiO 2 or modified gold surface [7] .…”

Section: Introductionmentioning

confidence: 99%

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Polymer Nanodiscs and Their Bioanalytical Potential

Farrelly

Martin

Thang

2021

Chemistry A European J

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Membrane proteins (MPs) play a pivotal role in cellular function and are therefore predominant pharmaceutical targets. Although detailed understanding of MP structure and mechanistic activity is invaluable for rational drug design, challenges are associated with the purification and study of MPs. This review delves into the historical developments that became the prelude to currently available membrane mimetic technologies before shining a spotlight on polymer nanodiscs. These are soluble nanosized particles capable of encompassing MPs embedded in a phospholipid ring. The expanding range of reported amphipathic polymer nanodisc materials is presented and discussed in terms of their tolerance to different solution conditions and their nanodisc properties. Finally, the analytical scope of polymer nanodiscs is considered in both the demonstration of basic nanodisc parameters as well as in the elucidation of structures, lipidprotein interactions, and the functional mechanisms of reconstituted membrane proteins. The final emphasis is given to the unique benefits and applications demonstrated for native nanodiscs accessed through a detergent free process.

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Section: Membrane Mimetic Technology: a Brief Historymentioning

confidence: 99%

Section: Membrane Mimetic Technology: a Brief Historymentioning

confidence: 99%

Section: Functionally Modified Smanh Derivatives: Smadsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Polymer Nanodiscs and Their Bioanalytical Potential

Farrelly

Martin

Thang

2021

Chemistry A European J

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“…Access to the neutron beam is limited and highly specialised personnel are needed for these experiments. Methodology based on attenuated total reflection Fourier transfer infrared spectroscopy (ATR-FTIR) seems an accessible complementary alternative method to determine lipid exchange [75], which is far more accessible and exists in many laboratories as a benchtop technique.…”

Section: Relative Lipid Exchange and Removal For Hdl Or Ldl Particlesmentioning

confidence: 99%

Structure of Lipoproteins and Their Capacity for Lipid Exchange: Relevance for Development of Atherosclerosis and Its Treatment by HDL Therapy

Waldie¹,

Giudice²,

Cárdenas³

2021

Management of Dyslipidemia

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Atherosclerosis, the largest killer in the western world, arises from build-up of plaques at the artery walls and can result in cardiovascular disease. Low- and high-density lipoproteins are involved in the disease development by depositing and removing lipids to and from macrophages at the artery wall. These processes are complex and not fully understood. Thus, determining the specific roles of the different lipoprotein fractions involved is of fundamental importance for the treatment of the disease. In this chapter, we present the state of the art in lipoprotein structure with focus on the comparison between normolipidemic and hypertriglyceridemic individuals. Then we discuss lipid transfer between lipoproteins and receptor-free cellular membranes. Although these models lack any receptor, key clinical observations are mirrored by these, including increased ability of HDL to remove lipids, in contrast to the ability of LDL to deposit them. Also effects of saturated and unsaturated lipids in the presence and absence of cholesterol are revised. These models can then be used to understand the difference in functionality of lipoproteins from individuals showing different lipid profiles and have the potential to be used also for the development of new HDL therapies.

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Interactions of Choline and Geranate (CAGE) and Choline Octanoate (CAOT) Deep Eutectic Solvents with Lipid Bilayers

Neville,

Dobre,

Smith

et al. 2023

Adv Funct Materials

Self Cite

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Mixtures between choline and geranic acid (CAGE) have previously been shown to insert into lipid bilayers. This may be useful for the transdermal delivery of larger pharmaceuticals, however, little is known about the mechanism of activity. By comparing the interactions between CAGE and lipid bilayers with those of a less‐active, yet closely‐related analogue, choline octanoic acid (CAOT), a chemical basis can be investigated. Overall, six systems are studied here by neutron reflectivity, where d54‐1,2‐dimyristoyl‐sn‐glycero‐3‐phosphocholine (DMPC) solid‐supported phospholipid bilayers are first formed on SiO2 substrates before exposure to the deep eutectic solvent (DES). Components of the DES could be identified within the bilayer by exploiting contrast variation and selective deuteration. CAGE is shown to be a mild disruptive agent, free to insert and diffuse across the bilayer, preserving much of the bilayer integrity. Experiments identify co‐mingling of geranate ions inhibits the efficient packing of lipid tails, increasing hydration across the bilayer. Conversely, CAOT is found to both exchange and remove lipid molecules to achieve incorporation, inducing swelling and the formation of solvent patches. It appears these behaviors derive from the structures of the anions and thus amphiphilicity of the DES, laying the foundations for the rational design and optimization of these candidates toward transdermal delivery.

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Adsorption of a styrene maleic acid (SMA) copolymer-stabilized phospholipid nanodisc on a solid-supported planar lipid bilayer

Abstract: Graphical abstract

Cited by 11 publications

References 52 publications

Polymer Nanodiscs and Their Bioanalytical Potential

Polymer Nanodiscs and Their Bioanalytical Potential

Structure of Lipoproteins and Their Capacity for Lipid Exchange: Relevance for Development of Atherosclerosis and Its Treatment by HDL Therapy

Interactions of Choline and Geranate (CAGE) and Choline Octanoate (CAOT) Deep Eutectic Solvents with Lipid Bilayers

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