Adsorption and viscoelastic properties of fractionated mucin (BSM) and bovine serum albumin (BSA) studied with quartz crystal microbalance (QCM-D)

Feiler, Adam; Sahlholm, Anna; Sandberg, Tomas; Caldwell, Karin D.

doi:10.1016/j.jcis.2007.07.029

Cited by 171 publications

(187 citation statements)

References 36 publications

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“…The proposed model confirmed previous in situ studies on the adsorption of BSM at solid/liquid interfaces showing the presence of a loosely bound mucin layer in addition to irreversibly bound BSM [21]. In general, a surface bound BSM layer is considered to be much softer than a rigid layer of surface bound BSA [19].…”

Section: Lpo Adsorption On Au-bsm Surfacesupporting

confidence: 86%

“…A noticeable compaction of the protein bi-layer may be expected by the incorporation of LPO molecules into the mucin layer. Similar compaction behaviour of a surface bound BSM layer was previously observed upon subsequent addition of BSA [19] or chitosan [22]. The adsorption conditions might be of high importance in determining the interactions between biopolymers at surfaces since a swelling of an adsorbed BSM layer upon subsequent adsorption of chitosan has been also reported [23,24].…”

Section: Lpo Adsorption On Au-bsm Surfacementioning

confidence: 52%

“…It is known that from synergistic interactions BSM can form complexes with other proteins [18,19]. Castillo et al [20] have proposed the three-layer model for the in vitro bovine mucin adsorption on the surface of a contact lens with the top layer consisting of BSM gel layers capable of desorption.…”

Section: Lpo Adsorption On Au-bsm Surfacementioning

confidence: 99%

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Activity of lactoperoxidase when adsorbed on protein layers

Haberska

Svensson

Shleev

et al. 2008

Talanta

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This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with colleagues.Other uses, including reproduction and distribution, or selling or licensing copies, or posting to personal, institutional or third party websites are prohibited. Contents lists available at ScienceDirect Talanta j o u r n a l h o m e p a g e : w w w . e l s e v i e r . c o m / l o c a t e / t a l a n t a b s t r a c tLactoperoxidase (LPO) is an enzyme, which is used as an antimicrobial agent in a number of applications, e.g., food technology. In the majority of applications LPO is added to a homogeneous product phase or immobilised on product surface. In the latter case, however, the measurements of LPO activity are seldom reported. In this paper we have assessed LPO enzymatic activity on bare and protein modified gold surfaces by means of electrochemistry. It was found that LPO rapidly adsorbs to bare gold surfaces resulting in an amount of LPO adsorbed of 2.9 mg/m 2 . A lower amount of adsorbed LPO is obtained if the gold surface is exposed to bovine serum albumin, bovine or human mucin prior to LPO adsorption. The enzymatic activity of the adsorbed enzyme is in general preserved at the experimental conditions and varies only moderately when comparing bare gold and gold surface pretreated with the selected proteins. The measurement of LPO specific activity, however, indicate that it is about 1.5 times higher if LPO is adsorbed on gold surfaces containing a small amount of preadsorbed mucin in comparison to the LPO directly adsorbed on bare gold.

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Section: Lpo Adsorption On Au-bsm Surfacesupporting

confidence: 86%

Section: Lpo Adsorption On Au-bsm Surfacementioning

confidence: 52%

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Activity of lactoperoxidase when adsorbed on protein layers

Haberska

Svensson

Shleev

et al. 2008

Talanta

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“…In line with alternatives (b) and (c), we therefore tentatively propose a synergistic action of the mucin and serum components on the surface, wherein the mucin component could provide favorable support for the serum components. In support of such synergistic action, recent studies on a BSMbovine serum albumin system have shown several biologically relevant parameters, such as the layer viscoelasticity [33] and the interfacial friction and layer stability (unpublished data) to be significantly changed for the composite layer compared to the layers of its constituting components. As described there [33], the addition of albumin resulted in a more rigid, presumably more compact surface layer, which could resist substantial shear.…”

Section: Discussionmentioning

confidence: 91%

Interactions between human neutrophils and mucin-coated surfaces

Sandberg

Carlsson

Ott

2008

J Mater Sci: Mater Med

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Recently, we showed microscopically that bovine (BSM), porcine (PGM) and human (MG1) mucin coatings could suppress the adhesion of neutrophils to a polyethylene terephthalate-based model biomaterial (Thermanox). Here, using the release of reactive oxygen species (ROS) as a marker of material-induced neutrophil activation, the strong surface-passivating effects of these mucin coatings were corroborated. Under optimal adsorption conditions, all mucin species performed equally well, thus indicating a high degree of functional homology between the mucins. Cell adhesion and morphology correlated well with the release of ROS. Quartz crystal microbalance (QCM-D) analysis linked low neutrophil activation to efficient mucin surface-shielding. Interestingly, the shielding power appeared equal for thick expanded and thin compact mucin coatings. Combined mucin-serum coatings were found to be highly surface-passivating. Particularly, since our data suggested partly synergistic mucin-serum action, we highlight the possibility that pre-adsorbed mucins could provide favorable support for adsorbing host components.

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“…[27,33] The QCM-D technique is thoroughly described by Höök; [34] the underlying physical principle is that the changes in frequency, (Δf), depend on the difference in the total oscillating mass, both that directly adsorbed and the solvent associated with the bound molecules. If the film is thin and rigid one can relate the decrease in frequency to the mass of the film according to the Sauerbrey relation.…”

Section: Qcm-d Measurements and Data Analysismentioning

confidence: 99%

Polymeric Smart Coating Strategy for Titanium Implants

et al. 2014

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Hyaluronan based hydrogel coatings can mimic extracellular matrix components and incorporate growth factors that can be released during a progressive degradation while new tissue regenerates. This paper describes a structural characterization of a hydrogel coating made of modified hyaluronan polymers and how these coatings interact with bone morphogenetic protein-2 (BMP-2). Quartz crystal microbalance and neutron reflectivity measurements were used for in-situ, real-time measurements of the adsorption properties of polymers and proteins on smooth titanium oxide surfaces that mimic implant products in orthopedics. The adsorption of BMP-2 on a bare titanium oxide surface is compared to that on titanium oxide coated with different chemically modified hyaluronan, the most important being hyaluronan with bisphosphonate groups (HA-BP). The subsequent release of the BMP-2 from these hydrogel coatings could be triggered by calcium ions. The amount of adsorbed protein on the surfaces as well as the amount of released protein both depend on the type of hyaluronan coating. We conclude that HA-BP coated titanium oxide surfaces provide an excellent material for growth factor delivery in-vivo.

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Adsorption and viscoelastic properties of fractionated mucin (BSM) and bovine serum albumin (BSA) studied with quartz crystal microbalance (QCM-D)

Cited by 171 publications

References 36 publications

Activity of lactoperoxidase when adsorbed on protein layers

Activity of lactoperoxidase when adsorbed on protein layers

Interactions between human neutrophils and mucin-coated surfaces

Polymeric Smart Coating Strategy for Titanium Implants

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