Adsorption and recognition of protein molecular imprinted calcium alginate/polyacrylamide hydrogel film with good regeneration performance and high toughness

Zhao, Kongyin; Tian, Chen; Lin, Beibei; Cui, Wenkui; Kan, Bohong; Yang, Ning; Zhou, Xiangyu; Zhang, Xinxin; Wei, Junfu

doi:10.1016/j.reactfunctpolym.2014.12.001

Cited by 41 publications

(17 citation statements)

References 37 publications

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“…However, there are also some challenges for bio‐macromolecules, especially proteins, due to the structural complexity, conformational flexibility, large molecular size and poor solubility in water . Accordingly, more promising alternative techniques have been exploited to overcome these barriers from different viewpoints . Surface imprinting is one of the most widely used methods, which locates all affinity recognition sites on the polymer surfaces; thus the diffusion of large macromolecules into and out of the network is facilitated.…”

Section: Introductionmentioning

confidence: 99%

Preparation of pH and temperature dual‐sensitive molecularly imprinted polymers based on chitosan and N‐isopropylacrylamide for recognition of bovine serum albumin

Dong

Hou

et al. 2019

Polymer International

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pH and temperature dual‐sensitive protein imprinted microspheres with high absorption capacity have been successfully synthesized on the surface of SiO2 using chitosan grafted N‐isopropylacrylamide (CS‐g‐NIPAM) as the pH and temperature sensitive monomer, with acrylamide as comonomer, N,N′‐methylenebisacrylamide as the crosslinking agent and bovine serum albumin (BSA) as the template protein. The pH and temperature dual‐sensitivity was also investigated. The results showed that the adsorption capacity and imprinting factor improved slowly with increasing incubation pH from 4.6 to 7.0, and then decreased sharply in alkaline conditions due to the reduction of non‐specific binding from electrostatic and hydrogen bonding interactions. Fourier transform infrared spectroscopy, thermogravimetric analysis and transmission electron microscopy were used to characterize the polymers. The as‐prepared SiO2@BSA molecularly imprinted polymers were also found to have high adsorption capacity (119.88 mg g−1) within 2 h, an excellent imprinting factor (α = 2.25), specific selectivity and good reusability. © 2019 Society of Chemical Industry

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Section: Introductionmentioning

confidence: 99%

Preparation of pH and temperature dual‐sensitive molecularly imprinted polymers based on chitosan and N‐isopropylacrylamide for recognition of bovine serum albumin

Dong

Hou

et al. 2019

Polymer International

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“…Alginic acid is abundantly obtained as a marine biological resource, being especially produced by brown-algae seaweed, and alginate has been widely used in food industry [21], pharmaceutical and tissue engineering [22][23][24]. Our previous effort has focused on the preparation of a free-standing calcium alginate (CaAlg) filtration membrane using polyethylene glycol (PEG) as porogen agent [25].…”

Section: Introductionmentioning

confidence: 99%

Removal of Dyes and Cd2+ in Water by Kaolin/Calcium Alginate Filtration Membrane

Zhao

Liu

et al. 2019

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Kaolin/calcium alginate (kaolin/CaAlg) free-standing membranes were prepared by adding different amounts of Kaolin into the sodium alginate (NaAlg) casting solution and crosslinked by Ca2+ using urea as porogen agent. The morphology of the kaolin/CaAlg filtration membrane was characterized by scanning electron microscope (SEM). Then the kaolin/CaAlg membrane was used for the removal of dyes and Cd2+. The kaolin significantly improved the mechanical behavior and flux of the kaolin/CaAlg membrane. The flux reached 17.53 L/m2·h at 0.1 MPa and when the content of Kaolin in NaAlg was 70 wt.%. The filtration of BSA solution and oil-water emulsion indicated that the kaolin/CaAlg composite filtration membrane exhibited good anti-fouling properties. The rejection of Brilliant Blue G250, Congo red, and methylene blue by the kaolin/CaAlg filtration membrane was 100%, 95.22%, and 62.86%, respectively. The removal rate of Cd2+ reached 99.69%, with a flux of 17.06 L/m2·h at 0.1 MPa.

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“…[13][14][15] Template molecules interact with functional monomers to produce stable polymers by intermolecular interactions that utilize the chemical cross-linking reaction. By eluting the template molecules, a molecular imprinted polymer (MIP) is produced which retains binding sites and holes [16][17][18][19][20] that exactly match the size and shape of the original template, and such structures are like locks and keys. MIPs prepared by conventional methods exhibit high specic selectivity, strong stability and wide utilizations in chromatographic separation, solid phase extraction, simulated enzyme catalysis and membrane separation.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of switchable intelligent molecularly imprinted polymers with selective adsorption of ethyl carbamate and their application in electrochemical sensor analysis

et al. 2018

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Adsorption and recognition of protein molecular imprinted calcium alginate/polyacrylamide hydrogel film with good regeneration performance and high toughness

Cited by 41 publications

References 37 publications

Preparation of pH and temperature dual‐sensitive molecularly imprinted polymers based on chitosan and N‐isopropylacrylamide for recognition of bovine serum albumin

Preparation of pH and temperature dual‐sensitive molecularly imprinted polymers based on chitosan and N‐isopropylacrylamide for recognition of bovine serum albumin

Removal of Dyes and Cd2+ in Water by Kaolin/Calcium Alginate Filtration Membrane

Synthesis of switchable intelligent molecularly imprinted polymers with selective adsorption of ethyl carbamate and their application in electrochemical sensor analysis

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