Adsorption and mass transfer studies of Catalase in IMAC chromatography by dynamics methods

Gutiérrez, R.; Valle, Eva M. Martín del; Galán, Miguel Á.

doi:10.1016/j.procbio.2005.06.014

Cited by 7 publications

(8 citation statements)

References 21 publications

(19 reference statements)

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“…However, more than one type of interaction mechanism is operational. 32 The major functional groups on protein contributing toward the interaction with chelated metal ion consist of the histidine residue and the sulfur atom of the sulfidryl group of the free cysteine residue. While maintaining a free cysteine residue in a natural protein is rare, the exposed histidine residue is the dominant binding site in protein adsorption with a chelated metal ion.…”

Section: Effect Of Metal Typementioning

confidence: 99%

Antibody purification from human plasma by metal‐chelated affinity membranes

Yavuz

Bereli

Armutçu

et al. 2011

J of Applied Polymer Sci

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The aim of this study is to investigate in detail the feasibility of poly(2-hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine methyl ester), PHEMAH membranes for purification of immunoglobulin G (IgG) from human plasma. PHEMAH membranes were prepared by photo-polymerization technique. Then, Zn 2þ , Ni 2þ , Co 2þ , and Cu 2þ ions were chelated directly on the PHEMAH membranes. Elemental analysis assay was performed to determine the nitrogen content and polymerized MAH was calculated as 168.5 lmol/g. The nonspecific IgG adsorption onto the plain PHEMA membranes was negligible (about 0.25 mg/mL). A remarkable increase in the IgG adsorption capacities were achieved from human plasma with PHEMAH membranes (up to 68.4 mg/mL).Further increase was observed with the metal-chelated PHEMAH membranes (up to 118 mg/mL). The metal-chelate affinity membranes allowed the one-step separation of IgG from human plasma. The binding range of metal ions for surface histidines from human plasma followed the order: Cu 2þ > Ni 2þ > Zn 2þ > Co 2þ . Adsorbed IgG was eluted using 250 mM EDTA with a purity of 94.1%. IgG molecules could be repeatedly adsorbed and eluted with the metal-chelated PHEMAH membranes without noticeable loss in their IgG adsorption capacity.

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Section: Effect Of Metal Typementioning

confidence: 99%

Antibody purification from human plasma by metal‐chelated affinity membranes

Yavuz

Bereli

Armutçu

et al. 2011

J of Applied Polymer Sci

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“…Three main steps determine the adsorption process in a packed bed-column: the solute transport and dispersion in the moving phase, the mass transfer between the solid phase and the moving phase, and the solute transport, diffusion and adsorption in the stationary phase [6]. Between these steps, it must determine which of them is/are controlling steps.…”

Section: Adsorption-controlling Stepmentioning

confidence: 99%

“…(t/q t ) = (1/k 2 q 2 2cal ) + (1/q 2cal )t (6) where k 2 is the equilibrium rate constant of pseudo-second-order adsorption (g/mg min) and q 2cal is the adsorption capacity calculated by the pseudo-second-order kinetic model (mg/g). According to the values in Table 3, the optimum results are for both the second and first order models, with the second order mechanism R 2 values being the highest.…”

Section: Adsorption-controlling Stepmentioning

confidence: 99%

“…Aromatic amino acids and the amino-terminal of the peptides also have some contributions. The benefits of IMAC -ligand stability, relatively high selectivity, high protein loading, mild elution conditions, simple regeneration, low cost of metal ions, and reuse of adsorbents for hundred of times without and detecable loss of metal-chelating properties -are decisive when developing large-scale purification processes for industrial applications [6].…”

Section: Introductionmentioning

confidence: 99%

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Synthesis of tentacle type magnetic beads as immobilized metal chelate affinity support for cytochrome c adsorption

Türkmen

Yavuz

Deni̇zli̇

2006

International Journal of Biological Macromolecules

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“…When these amino acids are present on the surface of an protein such as histidine (His) − a highly selective and reversible adsorption process takes place . The traditional approach to packing of affinity chromatography columns is a random bed of solid particles . Random particle beds demand large pressure gradients due to enhanced tortuosity and small void fractions.…”

Section: Introductionmentioning

confidence: 99%

Productivity Model for Separation of Proteins Using Ceramic Monoliths As a Stationary Phase

Vega

Valle

Cerro

et al. 2014

Ind. Eng. Chem. Res.

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The compact geometry and outstanding mechanical properties of ceramic monoliths are valuable features when designing reusable standard affinity chromatography columns. High throughput protein separations are becoming standard industrial practice. Column reuse is an important consideration for the development of separation processes when production rate per unit volume of column is taken into account. Due to the cost and downtime needed to regenerate chromatographic columns, throughput depends on the use of the same column for several adsorption–desorption cycles without a significant loss in adsorption. An adsorption–desorption dynamic model was developed and used to estimate the optimum reuse of ceramic monolith columns in affinity chromatography. The model experimental metal chelate affinity chromatography (IMAC) system is a ceramic monolith covered with agarose type D-5 activated with 1.4 butanediol diglycidyl ether as spacer arm, iminodiacetic acid as chelating agent, and Cu2+ as ligand. The effect of performing several adsorption/elution cycles on column performance was evaluated using five monoliths at different flow rates, for a total of five cycles each. The strategy used to interpret experimental results leads to a theoretical model for successive monolith reuses that can be readily used to determine the optimal reuse of monoliths on the basis of maximum production rate per volume of chromatographic columns.

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Adsorption and mass transfer studies of Catalase in IMAC chromatography by dynamics methods

Cited by 7 publications

References 21 publications

Antibody purification from human plasma by metal‐chelated affinity membranes

Antibody purification from human plasma by metal‐chelated affinity membranes

Synthesis of tentacle type magnetic beads as immobilized metal chelate affinity support for cytochrome c adsorption

Productivity Model for Separation of Proteins Using Ceramic Monoliths As a Stationary Phase

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