Adsorption and catalytic activity of Porcine pancreatic lipase on rod-like SBA-15 mesoporous material

Immobilization of Candida rugosa lipase on poly(3-hydroxybutyrate-co-hydroxyvalerate): a new eco-friendly support

“…In other words, immobilized CRL has increased tolerance to heat compared with free CRL. Similar results were reported by Tutar et al [31] for CRL immobilized on sporopollenin and Li et al [13] for PPL immobilized on rod-like SBA-15. One of the main reasons for enzyme immobilization is the anticipated increase in enzyme stability.…”

“…NMR analysis 13 C NMR spectra of the support (PHBV) and the immobilized biocatalyst (PHBV-CRL) also showed similar behavior, with a small displacement and decrease in peaks seen for the immobilized biocatalyst compared with the support, as shown in the supplementary material (Online Resource 2). The chemical shifts associated with the resonance peaks observed in the spectrum of the PHBV support are similar to that reported by Slater et al [24], however a small peak at approximately 117 ppm was identified, which is probably due to the presence of impurities in the composition of the support.…”

“…Although somewhat decreased immobilized CRL activity was found with each successive reaction, this may be due to CRL leaching out from the surface of PHBV during the multiple soaking, separation, and washing with hexane steps employed during the recycling reaction, because the enzyme is only attached by weak interaction forces [32]. Li et al [13] reported that activity of PPL immobilized on rod-like SBA-15 mesoporous material by adsorption decreased with reuse, retaining 32% of its original activity after five reuses. Huang et al [11] also reported decreased activity with covalent immobilization of Candida rugosa lipase on a cellulose nanofiber membrane, the remaining activity being about 30% after eight cycles of batch operation.…”

Covalent immobilization of porcine pancreatic lipase on carboxyl-activated magnetic nanoparticles: Characterization and application for enzymatic inhibition assays

“…Up to date, PPL has been immobilized with various solid supports including macroporous anion exchange resin, magnetic microspheres, chitosan beads, porous silica beads and polyvinyl alcohol [3,7,8]. The critical shortcoming of physical adsorption is that the immobilized enzymes are easily leached out from porous materials, resulting in enzyme loss and poor operational stability [9,10]. Covalent immobilization can bind enzymes to the solid supports stably, thus preventing leakage of the enzymes.…”

Screening of supports for immobilization of commercial porcine pancreatic lipase

“…This fact can be related to the higher chemical affinities of the enzyme by these supports, as already demonstrated by some works of the literature. The works by Li et al [25][26] showed that the free hydroxil groups present in the surface of these supports can form hydrogen bounds with the functional groups of aminoacids chains of the enzyme, promoting higher efficiency to the immobilization process. The van der Waals forces can also help the adsorption of the enzyme to the surface of the support.…”