Adrenergic regulation during acute hepatic infection with<i>Entamoeba histolytica</i>in the hamster: involvement of oxidative stress, Nrf2 and NF-KappaB

Aldaba‐Muruato, Liseth Rubí; Muñoz‐Ortega, Martín Humberto; Macías‐Pérez, José Roberto; Pulido-Ortega, Julieta; Martínez-Hernández, Sandra Luz; Ventura‐Juárez, Javier

doi:10.1051/parasite/2017048

Cited by 11 publications

(8 citation statements)

References 58 publications

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“…Previous studies have reported that Nrf2 is involved in the regulation of antioxidant responses and inflammation (21)(22)(23)(24). Western blotting demonstrated that the protein expression of Nrf2 and HO-1, which lies downstream of Nrf2, was further upregulated in osthole-treated BV2 microglia, compared with cells exposed to LPS alone (Fig.…”

Section: Effects Of Osthole On the Protein Expression Of Nrf2 And Ho-1mentioning

confidence: 71%

Protective effects of osthole against inflammation induced by lipopolysaccharide in BV2 cells

et al. 2018

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Inflammation and oxidative stress are implicated in the development of neurodegenerative diseases. Osthole is a compound that is extracted from She Chuang Zi, which is a type of traditional Chinese medicine. Osthole has previously been demonstrated to exhibit anticancer activities and has a low toxicity. However, to the best of our knowledge, the anti‑inflammatory effects of osthole in microglial cells have not been investigated extensively. The aim of the present study was to investigate the potential protective effects of osthole against inflammation induced by lipopolysaccharide (LPS) in microglial cells. The present study employed LPS‑stimulated BV2 mouse microglia to establish an inflammatory cell model and to investigate the anti‑inflammatory effects of osthole. Cells were pretreated with osthole for 1 h prior to LPS (10 µg/ml) stimulation. At 6 h after the addition of LPS, alterations in the levels of inflammatory factors, including tumor necrosis factor (TNF)‑α, interleukin (IL)‑6 and IL‑1β, were determined by ELISA. Furthermore, at 24 h after the addition of LPS, western blot analysis was performed to analyze the alterations in the protein expression of nuclear factor‑κB (NF‑κB) p65, phosphorylated‑NF‑κB p65, nuclear factor erythroid 2‑related factor 2 (Nrf2) and heme oxygenase (HO)‑1. The results demonstrated that the secretion of the inflammatory cytokines TNF‑α, IL‑6 and IL‑1β by LPS‑stimulated BV2 cells was significantly reduced by osthole treatment. Simultaneously, osthole treatment inhibited the LPS‑induced activation of the NF‑κB signaling pathway. In addition, osthole upregulated the expression of Nrf2 and HO‑1 in a dose‑dependent manner. Based on these results, osthole may exhibit anti‑inflammatory effects via the NF‑κB and Nrf2 pathways, indicating that osthole has the potential to be developed into an effective anti-inflammatory drug.

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Section: Effects Of Osthole On the Protein Expression Of Nrf2 And Ho-1mentioning

confidence: 71%

Protective effects of osthole against inflammation induced by lipopolysaccharide in BV2 cells

et al. 2018

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“…Oxidative stress is critically involved in the etiopathology of ALA [29]. There has been evidence that CUR exerts a hepatoprotective effect against oxidative stress via up-regulation of antioxidant enzyme genes [30,31].…”

Section: Resultsmentioning

confidence: 99%

Curcumin Attenuates the Pathogenicity of Entamoeba histolytica by Regulating the Expression of Virulence Factors in an Ex-Vivo Model Infection

et al. 2019

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Infection with the enteric protozoan Entamoeba histolytica is still a serious public health problem, especially in developing countries. Amoebic liver abscess (ALA) is the most common extraintestinal manifestation of the amoebiasis, and it can lead to serious and potentially life-threatening complications in some people. ALA can be cured by metronidazole (MTZ); however, because it has poor activity against luminal trophozoites, 40–60% of treated patients get repeated episodes of invasive disease and require repeated treatments that can induce resistance to MTZ, this may emerge as an important public health problem. Anti-virulence strategies that impair the virulence of pathogens are one of the novel approaches to solving the problem. In this study, we found that low doses of curcumin (10 and 50 μM) attenuate the virulence of E. histolytica without affecting trophozoites growth or triggering liver injury. Curcumin (CUR) decreases the expression of genes associated with E. histolytica virulence (gal/galnac lectin, ehcp1, ehcp5, and amoebapore), and is correlated with significantly lower amoebic invasion. In addition, oxidative stress is critically involved in the etiopathology of amoebic liver abscess; our results show no changes in mRNA expression levels of superoxide dismutase (SOD) and catalase (CAT) after E. histolytica infection, with or without CUR. This study provides clear evidence that curcumin could be an anti-virulence agent against E. histolytica, and makes it an attractive potential starting point for effective treatments that reduce downstream amoebic liver abscess.

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“…Subsequently, the overproduced ROS is scavenged via the activation of Nrf2 [ 70 ]. The inactivation of Nrf2 signaling is reported to be beneficial for the overactivation of NF- κ B [ 71 ].…”

Section: Discussionmentioning

confidence: 99%

The Antidiabetic and Antinephritic Activities ofTuber melanosporumvia Modulation of Nrf2-Mediated Oxidative Stress in the db/db Mouse

Jiang

Teng

Wang

et al. 2018

Oxidative Medicine and Cellular Longevity

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Tuber melanosporum (TM), a valuable edible fungus, contains 19 types of fatty acid, 17 types of amino acid, 6 vitamins, and 7 minerals. The antidiabetic and antinephritic effects of TM and the underlying mechanisms related to oxidative stress were investigated in db/db mice. Eight-week oral administration of metformin (Met) at 0.1 g/kg and TM at doses of 0.2 and 0.4 g/kg decreased body weight, plasma glucose, serum levels of glycated hemoglobin, triglyceride, and total cholesterol and increased serum levels of high-density lipoprotein cholesterol in the mice, suggesting hypoglycemic and hypolipidemic effects. TM promoted glucose metabolism by increasing the levels of pyruvate kinase and hepatic glycogen. It also regulated the levels of inflammatory factors and oxidative enzymes in serum and/or the kidneys of the mice. Additionally, TM increased the expression of nuclear respiratory factor 2 (Nrf2), catalase, heme oxygenase 1, heme oxygenase 2, and manganese superoxide dismutase 2 and decreased the expression of protein kinase C alpha, phosphor-janus kinase 2, phosphor-signal transducer and activator of transcription 3, and phosphor-nuclear factor-κB in the kidneys. The results of this study reveal the antidiabetic and antidiabetic nephritic properties of TM via modulating oxidative stress and inflammation-related cytokines through improving the Nrf2 signaling pathway.

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Adrenergic regulation during acute hepatic infection withEntamoeba histolyticain the hamster: involvement of oxidative stress, Nrf2 and NF-KappaB

Cited by 11 publications

References 58 publications

Protective effects of osthole against inflammation induced by lipopolysaccharide in BV2 cells

Protective effects of osthole against inflammation induced by lipopolysaccharide in BV2 cells

Curcumin Attenuates the Pathogenicity of Entamoeba histolytica by Regulating the Expression of Virulence Factors in an Ex-Vivo Model Infection

The Antidiabetic and Antinephritic Activities ofTuber melanosporumvia Modulation of Nrf2-Mediated Oxidative Stress in the db/db Mouse

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