Adrenergic Mechanisms

Andén, Nils‐Erik; Carlsson, Arvid; Häggendal, Jan

doi:10.1146/annurev.pa.09.040169.001003

Cited by 235 publications

(34 citation statements)

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“…Levels of CA and tyrosine hydroxylase (TH) activity were measured by HPLC/ED according to Mena et al (1989). CA turnover was evaluated according to the rate of depletion of CA after inhibition of their synthesis for I hour by alpha-methyl-paratyrosine (AMT), 250 rag/liter (Anden et al, 1969). The depletion rate of CA was measured by the difference between the levels of catecholamines before and after inhibition of TH by AMT.…”

Section: Studies With Neuroblastoma Cells Nb69mentioning

confidence: 99%

Effects of retinoic acid on NB 69 human neuroblastoma cells and fetal rat mid brain neurons

Mena¹,

Casarejos²,

Estrada

et al. 1994

J Neural Transm Gen Sect

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Retinoids are chemical compounds which play important roles in ontogenetic development and cranio-caudal differentiation in animals, but their effect on phenotypic expression of neurotransmitters are unknown. We studied the pharmacological and morphological effects of retinoic acid (RA) on two types of immature vertebrate neurons, the human derived neuroblastoma cells, NB69, and fetal rat mid brain neurons in culture. The pharmacological effects of RA on the cultures and their relation to catecholamine and acetylcholine neurotransmission were evaluated according the levels of catecholamines, tyrosine hydroxylase (TH) activity, TH immunostaining, and choline acetyltransferase (CAT) activity, respectively. RA reduces catecholamine levels and TH activity in NB69 cells and the number of dopamine neurons in cultures derived from rat fetal mid brain. The detrimental effect of RA on mid brain neurons is dose- dependent; limited to TH+ cells at low concentrations (100 to 500 nM) and toxic for all types of cells at high concentrations (1 to 2 microM). RA increases CAT activity in NB 69 cells and produces phenotypic differentiation of these to a more mature neuronal phenotype with more prolonged neurite extensions. Therefore, RA may play a trophic positive role in the differentiation of immature cells to cholinergic neurons; this contrasts with the detrimental effects of RA on catecholamine neurons.

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Section: Studies With Neuroblastoma Cells Nb69mentioning

confidence: 99%

Effects of retinoic acid on NB 69 human neuroblastoma cells and fetal rat mid brain neurons

Mena¹,

Casarejos²,

Estrada

et al. 1994

J Neural Transm Gen Sect

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“…When DOPA and NA were applied to the (c) Recovery of responses, 6.5 min after the application of propranolol had been terminated. (Johnson, Roberts, Sobieszek & Straughan, 1969;Bradshaw, Roberts & Szabadi, 1971;Bevan et al, 1974 (Anden et al, 1969;Anden, Engel & Rubenson, 1972a) and the stimulant effects of DOPA on locomotor activity (Corrodi, Fuxe, Ljungdahl & Ogren, 1970). Since these actions of DOPA coincide with raised levels of NA in the CNS, and can be blocked by drugs which inhibit the synthesis of NA from DOPA, it is unlikely that DOPA exerts a direct action of its own in these test systems.…”

Section: Agonistic Effect Of Dopamentioning

confidence: 99%

“…DOPA is the metabolic precursor of dopamine, and it is generally assumed that DOPA exerts its therapeutic effect via the synthesis and release of dopamine in the caudate nucleus (Hornykiewicz, 1974). However, DOPA is also a precursor of noradrenaline (NA), and it has been suggested that in structures receiving a NA innervation, exogenously administered DOPA may cause the release of NA from presynaptic terminals and thus mimic the actions of NA on post-synaptic cells (Anden, Carlsson & Haggendal, 1969). In the experiments described here we compared the effects of microelectrophoretically applied DOPA and NA on single neurones in the cerebral cortex, since this structure is known to be innervated by NA-containing neurones (Fuxe, 1965).…”

Section: Introductionmentioning

confidence: 99%

The Action of Micro‐electrophoretically Applied 1‐3,4‐dihydroxyphenylalanine (Dopa) on Single Cortical Neurones

Bevan

Bradshaw

Szabadi

1976

British J Pharmacology

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1 The technique of microelectrophoresis was used in order to compare the actions of L-3,4-dihydroxyphenylalanine (DOPA) and noradrenaline on single neurones in the cerebral cortices of cats and rats. 2 DOPA could both excite and depress cortical neurones. Cells excited by DOPA were also excited by noradrenaline and cells depressed by DOPA were also depressed by noradrenaline. 3 In the case of both excitatory and depressant responses, DOPA appeared to be less potent than noradrenaline. 4 Responses to DOPA and noradrenaline could be antagonized by phentolamine and propranolol. Responses to acetylcholine were not affected. 5 Responses to acetylcholine, but not responses to DOPA, were antagonized by atropine. 6 The results indicate that locally applied DOPA may mimic the actions of noradrenaline on cortical neurones. Possible mechanisms for these effects of DOPA are discussed.

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“…DOPA is a metabolic precursor of dopamine, and it is generally believed that DOPA exerts its therapeutic effect via the release of dopamine in the caudate nucleus . However, DOPA is also known to be a precursor of noradrenaline (NA), and it has been suggested that in structures receiving a NA innervation, exogenously administered DOPA may cause the release of NA from pre-synaptic terminals and thus mimic the actions of NA on post-synaptic cells (Anden, Carlsson & Haggendal, 1969). We have used the technique of microelectrophoresis to compare the action of DOPA and NA on single neurones in the cerebral cortex, a structure rich in NA-containing terminals .…”

Section: ))mentioning

confidence: 99%

PROCEEDINGS OF THE British Pharmacological Society

1975

British J Pharmacology

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Adrenergic Mechanisms

Cited by 235 publications

References 0 publications

Effects of retinoic acid on NB 69 human neuroblastoma cells and fetal rat mid brain neurons

Effects of retinoic acid on NB 69 human neuroblastoma cells and fetal rat mid brain neurons

The Action of Micro‐electrophoretically Applied 1‐3,4‐dihydroxyphenylalanine (Dopa) on Single Cortical Neurones

PROCEEDINGS OF THE British Pharmacological Society

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