Adrenalectomy counteracts the local modulation of astroglial fibroblast growth factor system without interfering with the pattern of 6-OHDA-induced dopamine degeneration in regions of the ventral midbrain

Chadi, Gerson; Silva, Camila; Maximino, Jéssica Ruivo; Fuxé, Kjell; Silva, Gilberto Ochman da

doi:10.1016/j.brainres.2007.11.024

Cited by 19 publications

(25 citation statements)

References 85 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Primary hippocampal neurons were seeded into 96-well plates and treated with 6-OHDA (50, 100, 150, 200 and 400 μM) for 24 h with or without bFGF (10,20,40 and 80 ng/mL). After 24 h incubation, cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.…”

Section: Viability Assaymentioning

confidence: 99%

See 1 more Smart Citation

Inhibition of Endoplasmic Reticulum Stress is Involved in the Neuroprotective Effect of bFGF in the 6-OHDA-Induced Parkinson’s Disease Model

Cai¹,

Ye²,

Zhu³

et al. 2016

Aging and disease

View full text Add to dashboard Cite

Parkinson's disease (PD) is a progressive neurodegenerative disorder with complicated pathophysiologic mechanisms. Endoplasmic reticulum (ER) stress appears to play a critical role in the progression of PD. We demonstrated that basic fibroblast growth factor (bFGF), as a neurotropic factor, inhibited ER stress-induced neuronal cell apoptosis and that 6-hydroxydopamine (6-OHDA)-induced ER stress was involved in the progression of PD in rats. bFGF administration improved motor function recovery, increased tyrosine hydroxylase (TH)-positive neuron survival, and upregulated the levels of neurotransmitters in PD rats. The 6-OHDA-induced ER stress response proteins were inhibited by bFGF treatment. Meanwhile, bFGF also increased expression of TH. The administration of bFGF activated the downstream signals PI3K/Akt and Erk1/2 in vivo and in vitro. Inhibition of the PI3K/Akt and Erk1/2 pathways by specific inhibitors partially reduced the protective effect of bFGF. This study provides new insight towards bFGF translational drug development for PD involving the regulation of ER stress.

show abstract

Section: Viability Assaymentioning

confidence: 99%

“…Several studies have focused on the relevance of bFGF in the dopaminergic nigrostriatal system. Chadi et al reported that bFGF promotes dopaminergic cell survival in human fetal tissue strands transplanted into immunosuppressed 6-OHDA-lesioned rats [10]. Another group showed that bFGF protects against rotenone-induced dopaminergic cell death [11].…”

mentioning

confidence: 99%

Inhibition of Endoplasmic Reticulum Stress is Involved in the Neuroprotective Effect of bFGF in the 6-OHDA-Induced Parkinson’s Disease Model

Cai¹,

Ye²,

Zhu³

et al. 2016

Aging and disease

View full text Add to dashboard Cite

show abstract

“…Schwann cell FGF-2 and S100ß proteins were assessed by Western blot analysis 14 . The Schwann cells cultures were homogenized in PBS, pH 7.4, containing 1% NP40, 0.5% sodium deoxycholate, 1% sodium dodecyl sulfate, 1 mM ethylenediaminetetraacetic acid, 1 mM ethylene glycol tetraacetic acid, and 1% protease inhibitor cocktail (Sigma).…”

Section: Western Blot Analysis Of Cultured Schwann Cellsmentioning

confidence: 99%

S100ß and fibroblast growth factor-2 are present in cultured Schwann cells and may exert paracrine actions on the peripheral nerve injury

et al. 2008

Self Cite

View full text Add to dashboard Cite

PURPOSE: The neurotrophic factor fibroblast growth factor-2 (FGF-2, bFGF) and Ca++ binding protein S100ß are expressed by the Schwann cells of the peripheral nerves and by the satellite cells of the dorsal root ganglia (DRG). Recent studies have pointed out the importance of the molecules in the paracrine mechanisms related to neuronal maintenance and plasticity of lesioned motor and sensory peripheral neurons. Moreover, cultured Schwann cells have been employed experimentally in the treatment of central nervous system lesions, in special the spinal cord injury, a procedure that triggers an enhanced sensorymotor function. Those cells have been proposed to repair long gap nerve injury. METHODS: Here we used double labeling immunohistochemistry and Western blot to better characterize in vitro and in vivo the presence of the proteins in the Schwann cells and in the satellite cells of the DRG as well as their regulation in those cells after a crush of the rat sciatic nerve. RESULTS: FGF-2 and S100ß are present in the Schwann cells of the sciatic nerve and in the satellite cells of the DRG. S100ß positive satellite cells showed increased size of the axotomized DRG and possessed elevated amount of FGF-2 immunoreactivity. Reactive satellite cells with increased FGF-2 labeling formed a ring-like structure surrounding DRG neuronal cell bodies.Reactive S100ß positive Schwann cells of proximal stump of axotomized sciatic nerve also expressed higher amounts of FGF-2. CONCLUSION: Reactive peripheral glial cells synthesizing FGF-2 and S100ß may be important in wound repair and restorative events in the lesioned peripheral nerves.

show abstract

“…To do so, the membranes were incubated with primary antibody against beta-actin diluted at 1:30.000 (Sigma, USA) in TBS-T with 1% Bovine Serum Albumin (BSA) for 1 hour at room temperature, then washed twice for 10 minutes with TBS-T and incubated for 45 minutes at room temperature with the secondary antibody (anti-rabbit, HRP-conjugated, Amersham) diluted at 1:10.000 in TBS-T with 1% BSA. After these incubations, the membranes were washed as previously described, which was followed by reaction and film developing 11 . The films were quantified by optical densitometry using an image analyzing system, as described by Chadi et al Samples were diluted in lysis buffer to obtain 60 µg in 25 µL of buffer.…”

Section: Carboxytherapy Is a Technique Originated In 1930 Inmentioning

confidence: 99%

Effects of subcutaneous carbon dioxide on calcitonin gene related peptide and substance P secretion in rat skin

et al. 2014

Self Cite

View full text Add to dashboard Cite

PURPOSE:To investigate the subcutaneous injection of carbon dioxide (CO 2 ) on neuropeptides Calcitonin Gene-Related Peptide (CGRP) and Substance P (SP) secretion in rat skin. METHODS:Fifty-six Wistar-EPM rats were distributed in two groups: one for CGRP analysis, the other for SP analysis. Each group was subdivided into four subgroups: control (Cont), control with needle (ContNd), CO 2 injection (CO 2 Inj) and atmospheric air injection (AirInj) -with seven animals each. Sample analyses of partial skin were conducted by Western Blotting (WB). RESULTS:In SP group, there was a decrease in the amount of neuropeptides in subgroups CO 2 Inj and AirInj. Similarly, in CGRP group, there was a decrease in the amount of pro-CGRP neuropeptides (15 kDa) in subgroups CO 2 Inj and AirInj; Nevertheless, there was no decrease in the amount of CGRP (5 kDa) in any subgroups.CONCLUSION: Subcutaneous injection of CO 2 and atmospheric air decreased the amount of Substance P and pro-Calcitonin GeneRelated Peptide (15 kDa) neuropeptides in rat skin.

show abstract

Adrenalectomy counteracts the local modulation of astroglial fibroblast growth factor system without interfering with the pattern of 6-OHDA-induced dopamine degeneration in regions of the ventral midbrain

Cited by 19 publications

References 85 publications

Inhibition of Endoplasmic Reticulum Stress is Involved in the Neuroprotective Effect of bFGF in the 6-OHDA-Induced Parkinson’s Disease Model

Inhibition of Endoplasmic Reticulum Stress is Involved in the Neuroprotective Effect of bFGF in the 6-OHDA-Induced Parkinson’s Disease Model

S100ß and fibroblast growth factor-2 are present in cultured Schwann cells and may exert paracrine actions on the peripheral nerve injury

Effects of subcutaneous carbon dioxide on calcitonin gene related peptide and substance P secretion in rat skin

Contact Info

Product

Resources

About