ADP-Glucose Pyrophosphorylase Is Located in the Plastid and Cytosol in the Pulp of Tropical Banana Fruit (Musa acuminata)

Solis-Badillo, Elizabeth; Agama‐Acevedo, Edith; Tiessen, Axel; Valenzuela, José Ángel López; Bello‐Pérez, Luis A.

doi:10.1007/s11130-019-00788-w

Cited by 10 publications

(6 citation statements)

References 25 publications

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“…Furthermore, the tubers and stolons of potatoes are representative organs that accumulate amyloplasts (Naeem et al, 1997;Sagisaka, 2008). In the case of fruits, starch mainly accumulates during the "maturation" period, such as with banana (Solis-Badillo et al, 2020) and Italian arum (Bonora et al, 2000). For apples, in vitro experiments showed that the formation of amyloplasts occurs in the callus and endosperm (Sagisaka, 2008).…”

Section: Development Of Proplastids Into Leucoplasts: Non-greening Phenotypementioning

confidence: 99%

Diversity of Plastid Types and Their Interconversions

Choi

2021

Front. Plant Sci.

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Plastids are pivotal subcellular organelles that have evolved to perform specialized functions in plant cells, including photosynthesis and the production and storage of metabolites. They come in a variety of forms with different characteristics, enabling them to function in a diverse array of organ/tissue/cell-specific developmental processes and with a variety of environmental signals. Here, we have comprehensively reviewed the distinctive roles of plastids and their transition statuses, according to their features. Furthermore, the most recent understanding of their regulatory mechanisms is highlighted at both transcriptional and post-translational levels, with a focus on the greening and non-greening phenotypes.

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Section: Development Of Proplastids Into Leucoplasts: Non-greening Phenotypementioning

confidence: 99%

Diversity of Plastid Types and Their Interconversions

Choi

2021

Front. Plant Sci.

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“…Starch is an important index associated with fruit texture, for instance, the softness of guava [ 5 ], and smoothness and dry constancy of pumpkin [ 6 ]. Starch synthesis and degradation pathway are involved in the regulation of fruit quality [ 7 , 8 ]. Phosphoglucomutase (PGM), ADP-glucose pyrophosphorylase (AGPS) and starch synthase (StSy) are key regulatory enzymes responsible for starch biosynthesis [ 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…Starch synthesis and degradation pathway are involved in the regulation of fruit quality [ 7 , 8 ]. Phosphoglucomutase (PGM), ADP-glucose pyrophosphorylase (AGPS) and starch synthase (StSy) are key regulatory enzymes responsible for starch biosynthesis [ 8 , 9 , 10 ]. During starch metabolism, the phosphorylation of amylopectin is an essential step which requires close collaboration of dikinases, the glucan, water dikinase (GWD), and phosphoglucan, water dikinase (PWD) [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolic Profiling of Sugars and Organic Acids, and Expression Analyses of Metabolism-Associated Genes in Two Yellow-Peel Pitaya Species

Xie

Chen

et al. 2022

Plants

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Sugar and organic acids are important factors determining pitaya fruit quality. However, changes in sugars and acids, and expressions of metabolism-associated genes during fruit maturation of yellow-peel pitayas are not well-documented. In this study, metabolic and expression analyses in pulps of different fruit developmental stages of ‘Wucihuanglong’ (‘WCHL’, Hylocereus undatus) and ‘Youcihuanglong’ pitaya (‘YCHL’, Hylocereus megalanthus) were used to explore the sugar and organic acid metabolic process. Total phenols and flavonoids were mainly accumulated at S1 in pitaya pulps. Ascorbic acid contents of ‘WCHL’ pitaya were higher than that of ‘YCHL’ pitaya during fruit maturation. Starch was mainly accumulated at early fruit development stages while soluble sugars were rich in late stages. Sucrose, fructose, and glucose were the main sugar components of ‘YCHL’ pitaya while glucose was dominant in ‘WCHL’ pitaya. Malic and citric acids were the main organic acids in ‘WCHL’ and ‘YCHL’ pitayas, respectively. Based on the transcriptome analyses, 118 genes involved in pitaya sugar and organic acid metabolism were obtained. Results from the correlation analyses between the expression profiling of candidate genes and the contents of sugar and organic acid showed that 51 genes had a significant correlation relationship and probably perform key role in pitaya sugar and organic acid metabolism processes. The finding of the present study provides new information for quality regulation of pitayas.

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“…[ 8 ] The enzyme of UDPG pyrophosphorylase (UGPase, EC 2.7.7.9) degrades UDPG produced by SuS to provide glucose‐1‐phosphate (G1P) for the demand of metabolic processes. [ 9 ] The C fluxes (cleaved sucrose) provide energy and building blocks for metabolism in different cell compartments through glycolysis. [ 10 ] The enzyme of GAPDH is the central enzyme in glycolysis, catalyzing the phosphorylating‐coupled oxidation of glyceraldehyde 3 phosphate (Ga3P) [Ga3P + orthophosphate (Pi) + nicotinamide adenine dinucleotide (NAD + )↔1,3‐diphosphoglyceric acid (DPGA) + dihydronicotinamide adenine dinucleotide (NADH) + H + ], a process critical for the partitioning of triose‐phosphate between different cell compartments and metabolic pathways.…”

Section: Introductionmentioning

confidence: 99%

“…As a rate-limiting enzyme of starch synthesis, AGPase catalyzes the conversion of G1P to adenosinediphosphate glucose (ADPG). [9,11,12] It consists of two large (AGPase-L) and two slightly smaller (AGPase-S) subunits. [9] The product of AGPase, ADPG, as the substrate of granule-bound and soluble starch synthase (GBSS and SSS, EC 2.4.1.21), is further catalyzed to form amylose (AM) and AP respectively.…”

Section: Introductionmentioning

confidence: 99%

The Effect and Regulation Mechanism of Powdery Mildew on Wheat Grain Carbon Metabolism

et al. 2022

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The effects of powdery mildew on gene expressions and activities of carbon (C) metabolism enzymes in Zhengmai 103 (resistant) and Xi'nong 979 (susceptible) are studied. Under severe powdery mildew, expressions and activities of sucrose synthase, uridine‐diphosphate glucose pyrophosphorylase, glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH), and cytosolic α‐1,4‐glucan phosphorylase decrease at 20 or 25–30 days after anthesis, and total soluble sugars content also decreases. It indicates that the first part of C metabolism (sucrose degradation) changes and total C flux reduces. Meanwhile, decreases in expressions and activities of adenosine‐diphosphate glucose pyrophosphorylase (AGPase), soluble starch synthase (SSS), starch‐branching enzyme I and II (SBEI and SBEII), and plastidic α‐1,4‐glucan phosphorylase coincide with decreases in amylopectin content, whereas increases in granule‐bound starch synthase I (GBSSI) expression and activity coincide with increases in amylose content. It shows that the second part of C metabolism (starch synthesis) changes. Therefore, powdery mildew alters C metabolism. In addition, it also induces defense responses in grains, such as the up‐regulation of a defense‐related protein endogenous α‐amylase/subtilisin inhibitor. Since GAPDH, AGPase, SSS, SBEII, and GBSSI are regulated by redox, changes of C metabolism are mainly regulated by cellular redox status.

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ADP-Glucose Pyrophosphorylase Is Located in the Plastid and Cytosol in the Pulp of Tropical Banana Fruit (Musa acuminata)

Cited by 10 publications

References 25 publications

Diversity of Plastid Types and Their Interconversions

Diversity of Plastid Types and Their Interconversions

Metabolic Profiling of Sugars and Organic Acids, and Expression Analyses of Metabolism-Associated Genes in Two Yellow-Peel Pitaya Species

The Effect and Regulation Mechanism of Powdery Mildew on Wheat Grain Carbon Metabolism

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