Administration of Prednisolone intravenously or Dexamethason orally in random donors reveals equal results in granulocyte collection: a single centre experience

Abstract

Since cytokine--mobilization for granulocyte collections in random donors--is not licensed in Austria, we investigated in a prospective analysis the effects of prednisolone or dexamethasone (DXM) in 153 donors (35 women/118 men) and in the collected products. Either prednisolone (50 mg) intravenously 4 h before granulocyte collection or DXM (8 mg) orally 12 h before collection was given. Collections were performed with the Cobe Spectra (CardianBCT, Lakewood). We recorded a significantly higher percentage of granulocytes in the peripheral blood of the DXM. However, the collected number of granulocytes was equal in both groups.

Granulocyte collection using a novel apheresis system eases the procedure and provides concentrates of high quality

“…Most of our recipients are pediatric patients with a low body weight. Thus, volume reduction is necessary to avoid a transfusion overload in these patients . At our institution a product volume exceeding 500 mL must be divided into two parts before further processing.…”

“…In random donors 35 products were collected after mobilization treatment with either prednisolone (P; n = 14) intravenously at a dose of 50 mg 4 hours before collection or alternatively with dexamethasone (DXM; n = 21) at a dose of 8 mg orally 12 hours before the harvest (depending on donor's preference). As published previously with both pretreatments similar amounts of granulocytes (PMNs) can be collected . For random donors a donation frequency of four harvests/year is permitted by the Austrian health authorities as the limiting factor is a dose of P of 200 mg/year and DXM of 32 mg/year.…”

“…GCs (n = 59) were done as previously described . The collection volume was set up to a maximum of 3 mL/min and, if necessary, reduced to keep the product volume low in recipients with low body weight.…”

Transfusion thérapeutique de granulocytes : cinétique et aspects organisationnels autour d’un cas clinique

“…Le choix du corticoïde a aussi fait l'objet d'études. Il n'y a pas de différence entre la stimulation par 8 mg DEXAMETHASONE orale ou par 50 mg de PREDNISONE intra-veineuse (12). Les effets secondaires de la stimulation par corticostéroïdes ont fait l'objet d'une revue (13).…”

Characterization of buffy coat‐derived granulocytes for clinical use: a comparison with granulocyte colony‐stimulating factor/dexamethasone‐pretreated donor‐derived products

“…Nevertheless, there are data suggesting less infection‐related mortality after granulocyte transfusions . To obtain enough granulocytes for donation, donors are pretreated with granulocyte colony‐stimulating factor (G‐CSF) and dexamethasone, or with steroids alone for granulocyte mobilization from the bone marrow prior to leukapheresis (from the G‐CSF and dexamethasone‐pretreated donors: ‘GTX’). We have previously shown that these granulocytes function well , although there is a significant partial in vitro Candida killing defect .…”