Administration of leuprolide acetate, a GnRH agonist, improves urodynamic parameters in ovariectomized rats

Medina-Aguiñaga, Daniel; Muñoz, Alvaro; Luna, Maricela; Martínez-Moreno, Carlos G.; Quintanar‐Stephano, Andrés; Quintanar, J. Luis

doi:10.1002/nau.23505

Cited by 4 publications

(4 citation statements)

References 25 publications

(64 reference statements)

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“…Also, previous studies suggested that OVX can cause a decreased of blood flow around the bladder neck and urethra, mucosal and smooth muscle atrophy, increased collagen synthesis and distribution, and reduced contractility. We found that the contraction induced by the muscarinic receptor agonist carbachol was significantly reduced in the detrusor muscle strips of OVX rats, consistent with previous studies (2,5,7,23). Ach and ATP are the main neurotransmitters that contract the detrusor muscle.…”

Section: Author Contributionssupporting

confidence: 92%

“…Hale as increased frequency, incontinence, urgency and nocturia. The relationship between overactive bladder and menopause is not clear but the decrease in ovarian hormones affect both in muscle fibers and innervation of bladder tissue (5). Ovariectomy causes bladder mucosa and detrusor smooth muscle atrophy, reduced blood flow, decreased bladder compliance and contractility (20,21).…”

Section: Author Contributionsmentioning

confidence: 99%

“…Thus, symptoms associated with menopause include dysuria, urinary frequency, and a range of urinary disorders such as recurrent bladder infections that may contribute to the development of overactive bladder syndrome (OABS) (2,4). Menopause is a risk factor for OABS, with its prevalence increasing significantly after the age of 40, reaching 39% in women aged 76 (5). It has been proposed that estrogen deficiency plays an important role in the genesis OABS because hypoestrogenism can induce alterations both in muscle fibers and in the innervation of the bladder.…”

Section: Introductionmentioning

confidence: 99%

“…It has been proposed that estrogen deficiency plays an important role in the genesis OABS because hypoestrogenism can induce alterations both in muscle fibers and in the innervation of the bladder. Indeed, it has been reported that menopause causes changes in the density of sensory nerve fibers in the bladder, thus triggering aberrant contractile activity similar to bladder hyperactivity (5). Ovariectomized (OVX) rats are often used as animal models of postmenopausal period.…”

Section: Introductionmentioning

confidence: 99%

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Ovarektomize Dişi Sıçanlarda 1,1-Dimetilbiguanit Hidroklorid (Metformin)'in Detrüsör Kas Kontraktil Yanıtı Üzerine Etkileri

TURAN

Erdem

ERGENÇ

et al. 2022

Türk Diyab Obez / Turk J Diab Obes

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Aim: Menopause is defined as the depletion of the ovarian follicular reserve followed by the cessation of menstrual cycles. It has been reported that gonadal steroid hormones play an important role in bladder function in women. Changes in urine pattern including overactive bladder, stress incontinence and recurrent urinary tract infections occur as a result of menopause. 1,1-dimethylbiguanide hydrochloride, metformin, (MET) is an oral anti-diabetic drug used to reduce hepatic glucose production and peripheral insulin resistance. Recent studies have revealed that MET has a protective effects in diabetes induced bladder dysfunction. The aim of this study was to test the therapeutic potential of MET in detrusor contractile function of ovariectomized (OVX) female rats. Material and Methods: Bilateral ovariectomy was performed to eliminate endogenous gonadal steroids secretion. Four groups are designed with 8 animals in each group: Control, MET-administered control, OVX, and MET-administered OVX groups. MET (25 mg/ kg) was administered daily by oral gavage for 14 days. Contractile activity of isolated bladder muscle strips were evaluated in vitro organ bath. The contractile responses of detrusor strips were determined using different doses of carbachol (10-8-10-2M) and purinergic agonist ATP. The relaxation response of strips were determined by isoproterenol Results: The contractile responses of detrusor muscle strips to carbachol at doses 10-5-10-2 M were decreased in the OVX group compared to control and MET treated control groups. MET treatment partially reversed the reduction in OVX-induced contractile responses at 10-2 and 10 -3 M carbachol doses. There were no statistically significant difference in relaxation response between the experimental groups. Conclusion: Our findings suggest that treatment with MET could be the new potential therapeutic agent against bladder dyfunction in postmenopausal women. Further studies are needed for the therapeutic potential of MET in detrusor dysfunction induced by menopause.

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Section: Author Contributionssupporting

confidence: 92%

Section: Author Contributionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ovarektomize Dişi Sıçanlarda 1,1-Dimetilbiguanit Hidroklorid (Metformin)'in Detrüsör Kas Kontraktil Yanıtı Üzerine Etkileri

TURAN

Erdem

ERGENÇ

et al. 2022

Türk Diyab Obez / Turk J Diab Obes

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Preventive Effects of Gonadotropin‐Releasing Hormone Treatment on Urinary Bladder and Kidney Damage in Spinal Cord Injured Rats

Calderón‐Vallejo,

Hernández‐Jasso,

Martínez‐Moreno

et al. 2024

Neurourology and Urodynamics

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IntroductionOne of the main causes of a neurogenic bladder is spinal cord injury (SCI),(SCI), which induces little or no bladder reflex activity. Because of this alteration, there is an increased risk of developing urinary tract infections and kidney damage. Gonadotropin‐releasing hormone (GnRH) treatment has been shown to improve micturition in a rat model of SCI.AimThe present study was aimed at determining whether GnRH administration is capable to reduce bladder and kidney damage in rats with SCI.MethodsOvariectomized female Wistar rats were divided into three groups: sham, SCI with saline solution (SCI), and SCI treated with GnRH (SCI+GnRH) for 6 weeks. SCI was induced by compression at the T10 spinal level. At the end of the experiment, bladders and kidneys were processed for morphological and immunofluorescence analysis. For morphometric analysis, the thickness of the urothelium and the muscular layer of the bladder was measured, as well as the intensity of staining related to collagen in the kidney.ResultsAt the end of the experiment, all animals in the sham group showed normal urination (100%), in contrast, the percentage of untreated injured rats (SCI) that did not require manual stimulation for micturition was 19%, while the treated group (SCI+GnRH) was 68%. A significative increase in bladder weight, urothelial and muscle thickness, and collagen‐related coloration in the kidney was observed in SCI when compared to sham rats.ConclusionGnRH administration decreased damage to the urinary bladder and kidneys after SCI in rats. These results suggest that this hormone could be a potential preventive treatment for SCI patients at risk of neurogenic bladder and kidney damage.Trial RegistrationNot applicable.

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Leuprolide Acetate, a GnRH Agonist, Improves the Neurogenic Bowel in Ovariectomized Rats with Spinal Cord Injury

et al. 2019

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Administration of leuprolide acetate, a GnRH agonist, improves urodynamic parameters in ovariectomized rats

Abstract: the results suggest that systemic application of LA can improve bladder dysfunction in castrated rats, and perhaps considered as a treatment for overactive bladder conditions secondary to menopause.

Cited by 4 publications

References 25 publications

Ovarektomize Dişi Sıçanlarda 1,1-Dimetilbiguanit Hidroklorid (Metformin)'in Detrüsör Kas Kontraktil Yanıtı Üzerine Etkileri

Ovarektomize Dişi Sıçanlarda 1,1-Dimetilbiguanit Hidroklorid (Metformin)'in Detrüsör Kas Kontraktil Yanıtı Üzerine Etkileri

Preventive Effects of Gonadotropin‐Releasing Hormone Treatment on Urinary Bladder and Kidney Damage in Spinal Cord Injured Rats

Leuprolide Acetate, a GnRH Agonist, Improves the Neurogenic Bowel in Ovariectomized Rats with Spinal Cord Injury

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