Adjuvant effect of mushroom glucan and bovine lactoferrin upon Aeromonas hydrophila vaccination in catla, Catla catla (Hamilton)

Abstract

Mushroom glucan and bovine lactoferrin (Lf), known for their immunostimulatory potential, were used as adjuvant in conjunction with a formalin-killed Aeromonas hydrophila vaccine in catla, Catla catla. In vitro antigen-specific responsiveness of catla leucocytes and protective responses against experimental challenge with homologous antigen were monitored following immunization. Antigen-specific proliferation, 'macrophage activating factor' (MAF) production and antibody production were significantly higher in fish injected with glucan adjuvanted vaccine. Lf adjuvanted preparations showed a weak proliferative response and MAF production, although the antibody production was significantly higher than the controls. A good degree of protection was achieved with the glucan adjuvanted vaccine. However, in spite of producing significant anti-A. hydrophila antibody, Lf adjuvanted vaccine did not confer any protection following challenge with A. hydrophila. The potential of adjuvanticity of mushroom glucan and bovine Lf in intraperitoneal vaccination is discussed.

Immune responses and protection in catla (Catla catla) vaccinated against epizootic ulcerative syndrome

“…This might be due to polyclonal activation of leukocyte already sensitized with the antigen. Similar antigen-specific proliferative responses have also been reported in catla [24] and other fish species [25,26]. Significantly higher proliferative response by FE þ A group compared to the control group demonstrated higher efficacy of the vaccine when it was mixed with adjuvant.…”

“…The challenge study did not reveal a good degree of protection against A. invadans in all the vaccinated groups. Reports are available to draw relationship between antibody production and protection [24,33,34]. The present study showed significant antibody production but the protection against A. invadans challenge was not significant enough.…”

“…Significantly higher proliferative response by FE þ A group compared to the control group demonstrated higher efficacy of the vaccine when it was mixed with adjuvant. The efficiency of a vaccine is increased when administered along with adjuvant, have also been demonstrated earlier in catla after Aeromonas hydrophila vaccination [24]. Lower responses in other groups might suggest the lower degree of memory induction.…”

“…In all the cases there was a progressive increase in antibody production with the increasing days of vaccination. Increased antibody response was observed in different Indian major carps following vaccination [24,33]. However, measurement of antibody titre against A. invadans vaccination is very limited.…”

Potential use of bovine lactoferrin as adjuvant for the modulation of the specific immune response in rainbow trout (Oncorhynchus mykiss W.)

“…In Indian carp Catla catla (Hamilton 1822), bLF-adjuvanted vaccine against Aeromonas hydrophila is able to enhance the Ab agglutination titre, although this does not confer better protection following experimental challenge (Kamilya et al 2006).…”