Adjunct Automation to the Cellmate™ Cell Culture Robot

Bernard, Christopher J.; Connors, David; Barber, Lauren E.; Jayachandra, Sukhanya; Bullen, Andrew; Cacace, Angela

doi:10.1016/j.jala.2004.03.004

Cited by 12 publications

(6 citation statements)

References 8 publications

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“…た細胞構造物や，細胞だけをシート状に形成した細胞シートが開発されてきた (Imamura et al, 2016) )．これらの細胞は，従来，細胞処理施設(CPC : Cell Processing Center)と呼ばれるクリーンルームにおいて専門の知識を有する作業者による手作業で製造されており，このような細胞の製造費用は大きく，その効率化が望まれている．そこで，これらの細胞の培養に関する作業を多関節型ロボットにより行う自動細胞培養装置 (Bernard et al, 2004) ) (中嶋他，2014) Fig. 7 Flow rate of injection.…”

Section: 塞などの虚血性心疾患により損傷した心筋組織の修復のために，骨格筋芽細胞，間葉系幹細胞，心筋幹細胞，Es 細胞，Ips 細unclassified

Study on cell culture processing system to improve task efficiency (Efficiency improvement of the injecting task using a robot in a media change process)

Nonoyama

Yori

Sameshima

et al. 2019

Transactions of the JSME (In Japanese)

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In the field of regenerative medicine, cell processing is currently done manually. The process is labor intensive and expensive, and its efficiency must be improved. Automatic cell culture apparatuses equipped with a vertical articulated robot have been proposed recently. However, automating all tasks of cell processing complicates the system constitution. The present study aims to develop a simple and rational cell processing system by combining the tasks performed by a robot with those performed by a human. Herein, we first analyze each task in the cell processing operation, verify whether a task can be efficiently performed by a robot and automated equipment, and decide the combination of the tasks. In a previous paper, we improved the efficiency of the task of discarding spent culture media in a flask by using a robot arm in the media change process. In the present paper, by focusing on the media change process, we examine the efficiency improvement of the task of injecting culture media by using a robot. We propose the configuration of an inspiration/injection port and an algorithm to estimate the start time of injection end motion from the flow rate and conduct a verification experiment. Our results show that a robot can perform the injecting task more efficiently than a human. Moreover, the risk of dripping can be reduced by using a robot.

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Section: 塞などの虚血性心疾患により損傷した心筋組織の修復のために，骨格筋芽細胞，間葉系幹細胞，心筋幹細胞，Es 細胞，Ips 細unclassified

Study on cell culture processing system to improve task efficiency (Efficiency improvement of the injecting task using a robot in a media change process)

Nonoyama

Yori

Sameshima

et al. 2019

Transactions of the JSME (In Japanese)

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“…Since medium exchange is difficult, time‐consuming and prone to contamination, successful attempts have been made toward automation using laboratory robots, e.g. Cellmate TM (Bernard et al ., ; Kunitake et al ., ). Very recently, Andrade‐Zaldivar et al ., ) reported a 17‐fold increase in CFU after 8 days of expansion of UCB MNCs using roller bottles (Andrade‐Zaldivar et al ., ).…”

Section: Bioreactor Culture Systemsmentioning

confidence: 99%

Stem cell bioengineering strategies to widen the therapeutic applications of haematopoietic stem/progenitor cells from umbilical cord blood

Andrade

Santos

Silva

2013

J Tissue Eng Regen Med

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Umbilical cord blood (UCB) transplantation has observed a significant increase in recent years, due to the unique features of UCB haematopoietic stem/progenitor cells (HSCs) for the treatment of blood-related disorders. However, the low cell numbers available per UCB unit significantly impairs the widespread use of this source for transplantation of adult patients, resulting in graft failure, delayed engraftment and delayed immune reconstitution. In order to overcome this issue, distinct approaches are now being considered in clinical trials, such as double-UCB transplantation, intrabone injection or ex vivo expansion. In this article the authors review the current state of the art, future trends and challenges on the ex vivo expansion of UCB HSCs, focusing on culture parameters affecting the yield and quality of the expanded HSC grafts: novel HSC selection schemes prior to cell culture, cytokine/growth factor cocktails, the impact of biochemical factors (e.g. O2 ) or the addition of supportive cells, e.g. mesenchymal stem/stromal cell (MSC)-based feeder layers) were addressed. Importantly, a critical challenge in cellular therapy is still the scalability, reproducibility and control of the expansion process, in order to meet the clinical requirements for therapeutic applications. Efficient design of bioreactor systems and operation modes are now the focus of many bioengineers, integrating the increasing 'know-how' on HSC biology and physiology, while complying with the GMP standards for the production of cellular products, i.e. through the use of commercially available, highly controlled, disposable technologies.

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“…Robotic systems for automating cell culture [2]- [4] and PCR-based diagnosis [5]- [8] have been developed, but these primarily cater to containers with screw caps, leaving a gap for systems compatible with microtubes with press-type caps. According to our knowledge, there are limited examples of automated systems incorporating microtubes with press-type caps [9]- [11].…”

Section: Introductionmentioning

confidence: 99%

Manually operated microtube automatic capper/decapper system for clinical laboratory and biological laboratory personnel

Jinno,

Nonoyama,

SAKURAI

et al. 2024

Preprint

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Polymerase chain reaction (PCR) is an effective method for diagnosing infectious diseases and has been the primary method throughout the novel coronavirus disease (COVID-19) pandemic. PCR tests (from specimen collection to result acquisition) involve sample pretreatment, nucleic acid extraction, and PCR procedure. Automating the pretreatment process is crucial to mitigate the risk of infection for workers and to reduce the likelihood of sample contamination-triggered misdiagnosis, particularly when handling centrifuge tubes, cryopreservation tubes, and microtubes. Robotic systems have been engineered to automate cell culture and PCR-based diagnosis , predominantly designed for use with screw-capped containers. However, this leaves a notable gap in automation solutions for microtubes equipped with press-type caps. To address this gap, we developed a versatile microtube capper/decapper system. On the other hand, many tasks of manual operation using microtubes, which are routinely conducted in clinical tests and biological experiments, were performed. Despite the risks of contamination and infection derived from the manual handling of microtube caps, which can compromise diagnosis/experiment accuracy and worker safety, devices for manually opening and closing microtube caps without direct contact remain lacking. Therefore, leveraging the technology from the developed versatile microtube capper/decapper system for laboratory automation, we created a manually operated microtube equipped with an automatic capper/decapper system tailored for personnel in clinical and biological laboratories. In this study, we first examined the required specifications and prerequisites for a manual microtube capper/decapper and clarified the operating methods, operating procedures, operation environment, device size, accompanying functions, etc. Based on the required specifications and preconditions, we proceeded with the mechanical and control design of the conceptual model, manufactured a prototype, and confirmed its basic functions and performance. The compliant to the required specifications and preconditions and the usefulness of the proposed manual microtube capper/decapper were validated through various experiments and demonstrations. Because microtubes are used in various clinical tests and biological experiments, we believe that the proposed system can markedly reduce the workload for personnel across numerous clinical and biological laboratories.

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Adjunct Automation to the Cellmate™ Cell Culture Robot

Cited by 12 publications

References 8 publications

Study on cell culture processing system to improve task efficiency (Efficiency improvement of the injecting task using a robot in a media change process)

Study on cell culture processing system to improve task efficiency (Efficiency improvement of the injecting task using a robot in a media change process)

Stem cell bioengineering strategies to widen the therapeutic applications of haematopoietic stem/progenitor cells from umbilical cord blood

Manually operated microtube automatic capper/decapper system for clinical laboratory and biological laboratory personnel

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