Adjacent pol II and pol III promoters: transcription of the yeast retrotransposon Ty3 and a target tRNA gene

Kinsey, Philip T.; Sandmeyer, Suzanne

doi:10.1093/nar/19.6.1317

Cited by 63 publications

(64 citation statements)

References 40 publications

(28 reference statements)

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“…In the case of Ty3, integration of the element adjacent to at least one tRNA gene was found not to significantly disrupt expression of the tRNA gene [41]. Nevertheless, disruption of tRNA gene promoters did relieve an apparent repression of Ty3 gene expression suggesting that tRNA genes could potentially limit the activity of retrotransposons.…”

Section: Discussionmentioning

confidence: 94%

RNA polymerase III interferes with Ty3 integration

Connolly

Sandmeyer

1997

FEBS Letters

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Ty3, a gypsylike retrotransposon of budding yeast, integrates at the transcription initiation site of genes transcribed by RNA polymerase III (pol III). It was previously shown that integration in vitro requires intact promoter elements and the pol III transcription factors TFIIIB and TFIIIC. In order to test the effect of pol III on integration, increasing amounts of a pol Illcontaining fraction were added to Ty3 in vitro integration reactions. The pol Ill-containing fraction was inhibitory to integration. These results are consistent with a model where the Ty3 integration complex and pol III recognize similar features of the stable transcription complex and compete with each other for access to the transcription initiation site.

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Section: Discussionmentioning

confidence: 94%

RNA polymerase III interferes with Ty3 integration

Connolly

Sandmeyer

1997

FEBS Letters

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“…These patterns of infection bias could be interpreted as an evolutionary result that caused little or no reduction in the host's fitness. In cases like yeast Ty 3 insertions upstream to tRNA genes (Kinsey and Sandmeyer, 1991), there is room to speculate that, to some extent, the gradual improvement of sub-vitality, which in fact occurred, was due to the fact that selection acted in favor of molecular parasites that localized themselves where they would produce semi-lethality but not mortality. Moreover, there is also a negative selection in ectopic recombination with homologous units at different chromosomal sites (Langley et al, 1988).…”

Section: Transportable Elements Had Several Entrances Into Unicellulamentioning

confidence: 99%

Mini-opinion From unicellularity to multicellularity - molecular speculations about early animal evolution

Hf¹,

Mh²,

Sanabria³

2008

Genet. Mol. Res.

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ABSTRACT.A morphological, physiological, developmental, and genetic organization of great complexity ineluctably unfolded from relatively simple phenomena invested with enormous potential. Sometime long ago in the Protererozoic times, parasitic invasions caused lower evolutionary levels to integrate into higher-level selection. Therefore, we have a multi-level selection problem that ultimately revolves around the question of how natural selection among lower-level units acts to create higher-level units of selection, in which Darwinian competition among replicators ceases to be the foremost force. The first level relinquishes its independence for the benefit of a higher-level cooperative force that is now the criterion of fitness for the new transition in the evolutionary process.

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“…The spatial clustering was origi-nally discovered because proximity to tRNA genes leads to transcriptional silencing of nearby RNA polymerase II (pol II) promoters, termed tRNA gene mediated (tgm) silencing (Kinsey and Sandmeyer 1991;Hull et al 1994;Kendall et al 2000). The colocalization of the tRNA genes may also contribute to the elevated rate of recombination between active tRNA genes in yeast (Pratt-Hyatt et al 2006).…”

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confidence: 99%

Clustering of yeast tRNA genes is mediated by specific association of condensin with tRNA gene transcription complexes

et al. 2008

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The 274 tRNA genes in Saccharomyces cerevisiae are scattered throughout the linear maps of the 16 chromosomes, but the genes are clustered at the nucleolus when compacted in the nucleus. This clustering is dependent on intact nucleolar organization and contributes to tRNA gene-mediated (tgm) silencing of RNA polymerase II transcription near tRNA genes. After examination of the localization mechanism, we find that the chromosome-condensing complex, condensin, is involved in the clustering of tRNA genes. Conditionally defective mutations in all five subunits of condensin, which we confirm is bound to active tRNA genes in the yeast genome, lead to loss of both pol II transcriptional silencing near tRNA genes and nucleolar clustering of the genes. Furthermore, we show that condensin physically associates with a subcomplex of RNA polymerase III transcription factors on the tRNA genes. Clustering of tRNA genes by condensin appears to be a separate mechanism from their nucleolar localization, as microtubule disruption releases tRNA gene clusters from the nucleolus, but does not disperse the clusters. These observations suggest a widespread role for condensin in gene organization and packaging of the interphase yeast nucleus.[Keywords: tRNA gene; condensin; microtubules; nuclear organization; nucleolus] Supplemental material is available at http://www.genesdev.org.

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Adjacent pol II and pol III promoters: transcription of the yeast retrotransposon Ty3 and a target tRNA gene

Cited by 63 publications

References 40 publications

RNA polymerase III interferes with Ty3 integration

RNA polymerase III interferes with Ty3 integration

Mini-opinion From unicellularity to multicellularity - molecular speculations about early animal evolution

Clustering of yeast tRNA genes is mediated by specific association of condensin with tRNA gene transcription complexes

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