Adjacent assembly of self-assembled monolayers for the construction of selective bio-platforms

Ron, Ariel; Lee, Gwan Hyoung; Amar, Levy; Ghassemi, Saba; Hone, James

doi:10.1016/j.snb.2011.06.051

Cited by 3 publications

(3 citation statements)

References 39 publications

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“…Previous work showed that electron beam irradiation does not change surface topography but rather surface functionality, as determined by phase imaging with atomic force microscopy [31]. The exact chemical nature of the change induced by the focused electron beam on K-casein is not known; however, it is likely to show similarities to changes previously reported on self-assembled monolayers [33,38].…”

Section: Resultsmentioning

confidence: 84%

“…EBL has been utilized to fabricate patterns of proteins on surfaces, and some of these patterned surfaces were also tested and shown to be functional at the cellular level [18,[26][27][28][29][30][31][32][33][34][35]. In one approach the resulting patterns present a purely biochemical signal because there are no differences in rigidity and the patterns are basically flat because adhesive patches have a thickness of only 2 nm [31].…”

Section: Introductionmentioning

confidence: 99%

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Micrometer scale spacings between fibronectin nanodots regulate cell morphology and focal adhesions

Horzum¹,

Özdil²,

Pesen-Okvur³

2014

Mater. Res. Express

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Cell adhesion to extracellular matrix is an important process for both health and disease states. Surface protein patterns that are topographically flat, and do not introduce other chemical, topographical or rigidity related functionality and, more importantly, that mimic the organization of the in vivo extracellular matrix are desired. Previous work showed that vinculin and cytoskeletal organization are modulated by size and shape of surface nanopatterns. However, quantitative analysis on cell morphology and focal adhesions as a function of micrometer scale spacings of FN nanopatterns was absent. Here, electron beam lithography was used to pattern fibronectin nanodots with micrometer scale spacings on a Kcasein background on indium tin oxide coated glass which, unlike silicon, is transparent and thus suitable for many light microscopy techniques. Exposure times were significantly reduced using the line exposure mode with micrometer scale step sizes. Micrometer scale spacings of 2, 4 and 8 μm between fibronectin nanodots proved to modulate cell adhesion through modification of cell area, focal adhesion number, size and circularity. Overall, cell behavior was shown to shift at the apparent threshold of 4 μm spacing. The findings presented here offer exciting new opportunities for cell biology research.

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Section: Resultsmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Micrometer scale spacings between fibronectin nanodots regulate cell morphology and focal adhesions

Horzum¹,

Özdil²,

Pesen-Okvur³

2014

Mater. Res. Express

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“…The layer-by-layer (LbL) method [5,13–17] based on physisorption of alternating layers of positive and negatively charged species has been proven a useful tool to adjust physicochemical properties and improve biocompatibility and mechanical properties of materials for biological applications and tissue engineering [18–23]. In addition to being suitable for functionalizing surfaces with high degree of control, it also allows for bioactivity preservation of enzymes and proteins [24,25], with which applications can be developed in bioengineering, biotechnology and biosensing. Indeed, LbL films have been used to tune the mechanical strength, cell attachment and proliferation for musculoskeletal tissue engineering [13] and in scaffolds for controlled release of drugs [26].…”

Section: Introductionmentioning

confidence: 99%

Bending of Layer-by-Layer Films Driven by an External Magnetic Field

Miyazaki

Riul

Santos

et al. 2013

IJMS

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We report on optimized architectures containing layer-by-layer (LbL) films of natural rubber latex (NRL), carboxymethyl-chitosan (CMC) and magnetite (Fe3O4) nanoparticles (MNPs) deposited on flexible substrates, which could be easily bent by an external magnetic field. The mechanical response depended on the number of deposited layers and was explained semi-quantitatively with a fully atomistic model, where the LbL film was represented as superposing layers of hexagonal graphene-like atomic arrangements deposited on a stiffer substrate. The bending with no direct current or voltage being applied to a supramolecular structure containing biocompatible and antimicrobial materials represents a proof-of-principle experiment that is promising for tissue engineering applications in biomedicine.

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Heterodyne-detected sum frequency generation of water at surfaces with varying hydrophobicity

Sanders

Petersen

2019

The Journal of Chemical Physics

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Aqueous surfaces mediate many atmospheric, biological, and technological processes. At the interface, the bulk hydrogen-bonding network is terminated and the interfacial water molecules restructure according to the surface chemistry of the interface. Given the complexity of both natural and technical aqueous interfaces, self-assembled monolayers provide a platform for controllably tuning the chemical composition of the surface and thus the water restructuring. Here, we study a hydrophobic monolayer, a hydrophilic monolayer, and a mixed hydrophobic/hydrophilic monolayer in contact with water. Monolayers composed of both hydrophilic and hydrophobic chains mimic the complex and heterogeneous chemical composition of natural and technological surfaces. By employing heterodyne-detected sum frequency generation, the purely absorptive vibrational line shape of interfacial water is measured experimentally. We examined the structure of the interfacial water in contact with each of the monolayers by analyzing the relative dipole moment orientations and fitting the imaginary component of χ(2) with a combination of Lorentzian and Gaussian line shapes. For all of the monolayers, the hydrogen-bonded water points toward the monolayer, which is opposite of the orientation of the hydrogen-bonded water at the air-water interface. Additionally, a strongly hydrogen-bonded water species exists for the monolayers containing hydrophilic chains. The spectroscopic results suggest that the microscopic water structure in contact with the mixed monolayer is dominated by the hydrophilic parts of the monolayer, while the contact angle shows that at the macroscopic level the surface properties lie closer to the pure hydrophobic monolayer.

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Adjacent assembly of self-assembled monolayers for the construction of selective bio-platforms

Cited by 3 publications

References 39 publications

Micrometer scale spacings between fibronectin nanodots regulate cell morphology and focal adhesions

Micrometer scale spacings between fibronectin nanodots regulate cell morphology and focal adhesions

Bending of Layer-by-Layer Films Driven by an External Magnetic Field

Heterodyne-detected sum frequency generation of water at surfaces with varying hydrophobicity

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