Adipose tissue stromal vascular fraction in the treatment of full thickness burns in rats

Cardoso, Alexandre Lamaro; Bachion, Maria Márcia; Morais, Júlia de Miranda; Fantinati, Marcelo Silva; Almeida, Vera L.; Júnior, R.S. Lino

doi:10.1590/s0102-865020160090000002

Cited by 20 publications

(11 citation statements)

References 27 publications

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“…In summary, the potential antioxidant and anti-inflammatory effects of these two agents make the combination of SVFs and PRP a very promising, new and interesting therapeutic approach for burn patients. 30,31 This study showed that MDA and NO levels in the treatment group were lower than those in the negative control group (placebo). This indicates that the injection of both SVFs and PRP significantly reduced oxidative stress.…”

Section: Discussionmentioning

confidence: 86%

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The Combination of Stromal Vascular Fraction Cells and Platelet-Rich Plasma Reduces Malondialdehyde and Nitric Oxide Levels in Deep Dermal Burn Injury

Josh¹,

Soekamto²,

Adriani³

et al. 2021

JIR

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Introduction: Thermal burns release reactive oxygen species, which cause profound systemic and local changes. Stromal vascular fraction cells (SVFs) combined with platelet-rich plasma accelerate burn wound healing. This study investigated the effect of a combination of locally injected SVFs and PRP on malondialdehyde (MDA) and nitric oxide (NO) serum and tissue levels in a deep dermal burn model in Wistar rats. Methods: Thirty-six adult Wistar rats weighing between 150 and 250 grams were used in this study to establish a deep dermal degree burn wound model. They were randomly divided into 4 groups: locally injected the combination SVFs and PRP, the Vaseline group, the placebo group, and healthy Wistar rats (the normal control group). MDA and NO levels in blood serum and burn wound tissue were measured at 8, 24, and 48 hours. Data were analyzed with one-way ANOVA followed by multiple comparisons tests and regression tests. Results: Local injection of SVFs and PRP in combination affected blood MDA, tissue MDA, blood NO and tissue NO levels, with reductions of 0.257µmol/L, 0.427 µmol/L, 21.78nmol/mg, and 23.777nmol/mg, respectively. Injection of SVFs and PRP in combination reduced tissue MDA levels by 1.282 times, NO blood levels by 2.305, and NO tissue levels by 2.377 times compared to Vaseline application. Conclusion:The combination of SVFs and PRP undeniably reduced the MDA and NO levels in blood and tissue compared to those in the Vaseline and placebo groups. The injection of these two preparations in combination inhibited the local and systemic stress oxidative response, as illustrated by the decreased MDA and NO levels in blood serum and tissue.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

The Combination of Stromal Vascular Fraction Cells and Platelet-Rich Plasma Reduces Malondialdehyde and Nitric Oxide Levels in Deep Dermal Burn Injury

Josh¹,

Soekamto²,

Adriani³

et al. 2021

JIR

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“…Second, ADSCs can self-proliferate and have long-term survival and multiple differentiation potential, with more mesenchymal stem cells and stronger cell activity than bone marrow (Atalay et al 2014). Finally, compared with mesenchymal stem cells derived from other tissues, the cell viability of ADSCs is less affected by acquisition technology and patient's age and body mass index (Cardoso et al 2016).…”

Section: Discussionmentioning

confidence: 99%

The effect of adipose-derived stem cells in healing refractory wounds based on clinical outcomes

Yan

Jiang

et al. 2020

All Life

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Refractory wounds can be a serious threat to the quality of life and survival of patients. Various surgical methods have been attempted; however, they do not cure these refractory wounds; therefore, new and promising treatment methods to improve wound healing and angiogenesis are urgently warranted. Adipose-derived stromal vascular fraction (SVF) cells have been clinically used to promote healing. Hence, we aimed to evaluate the clinical efficacy of SVF cells for the treatment of refractory wounds. Forty patients with refractory wounds were randomly divided into two groups: patients in the experimental group were treated with SVFs (n = 20) and those in the control group were given conventional treatment (n = 20). The general data of age, sex ratio, wound size, frequency of every caused wound, and etiology were compared between the two groups. No significant difference was found between the two groups (P = 0.089). Clinical efficacy was also evaluated between the two groups. The experimental group had a shorter wound healing time and a significantly higher healing rate than the control group (P = 0.023 and P = 0.035, respectively). Therefore, SVFs may be a new and effective treatment for refractory wounds (P = 0.043).

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“…If the degree of inflammatory response in the body is moderate and the changes of inflammatory factor levels are limited, it is beneficial to protect the body and relieve body damage and infection. Conversely, it may cause secondary damage to the tissue, promoting disease development (17,18). In this study, the expression levels of miR-184 and miR-126 continuously decreased with the prolongation of time after burn, and the apoptosis degree in rats was getting higher and higher, indicating that the inflammatory response of rats continues to increase, and the secondary injury of burn wounds deepens.…”

Section: Discussionmentioning

confidence: 99%

Expression levels and significance of miR-184 and miR-126 in burned rats

Fan

et al. 2019

Exp Ther Med

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Expression levels and significance of miR-184 and miR-126 in burned rats were investigated. A total of 30 healthy rats were selected to construct a burn rat model, and another 10 healthy rats as the control group. The modeled rats were divided into groups I, II and III according to burn area, 10 for each group. The reverse transcription-quantitative polymerase chain reaction (RT-q.PCR) was used to detect the expression of miR-184 and miR-126 in the serum of three groups of burned rats, and ELISA was employed to detect the expression levels in peripheral blood and the correlation. There was no difference in the expression levels of miR-184 and miR-126 among the four groups of rats before modeling (P>0.05). Those of miR-184 and miR-126 at each time point were lower than those at the previous one in groups I, II and III (P<0.05). There was no significant change in the expression levels of miR-184 and miR-126 in the control group (P>0.05). At the same time point, those of miR-184 and miR-126 decreased with the increase of burn degree, and the difference was statistically significant between every two groups (P<0.05). The results of Pearson's correlation analysis revealed that the expression level of miR-184 was positively correlated with that of miR-126 (r=0.832, P=0.002). miR-184 and miR-126 were positively correlated with burn degree (r=0.901, P=0.001, r=0.775, P=0.001) and time after burn (r=0.732, P=0.004, r=0.753, P=0.002). The expression levels of miR-184 and miR-126 decrease in burned rats. The changes of their levels may be used as a reference standard for clinical efficacy evaluation to evaluate burn degree, preventing burn wounds from deepening.

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Adipose tissue stromal vascular fraction in the treatment of full thickness burns in rats

Cited by 20 publications

References 27 publications

The Combination of Stromal Vascular Fraction Cells and Platelet-Rich Plasma Reduces Malondialdehyde and Nitric Oxide Levels in Deep Dermal Burn Injury

The Combination of Stromal Vascular Fraction Cells and Platelet-Rich Plasma Reduces Malondialdehyde and Nitric Oxide Levels in Deep Dermal Burn Injury

The effect of adipose-derived stem cells in healing refractory wounds based on clinical outcomes

Expression levels and significance of miR-184 and miR-126 in burned rats

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