Adipose tissue extract promotes adipose tissue regeneration in an adipose tissue engineering chamber model

Lu, Zijing; Yuan, Yi; Gao, Jianhua; Lu, Feng

doi:10.1007/s00441-015-2322-5

Cited by 16 publications

(16 citation statements)

References 20 publications

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“…Over the past decade, adipose tissue is no longer considered to be just an energy‐storing fat pad, but also a storage of secretory factors, including cytokines, growth factors, and hormones . The discovery of secretory factors from adipose tissue has attracted interest on Adi‐CM, and several recent studies have demonstrated the beneficial effect of Adi‐CM in angiogenesis, wound healing, and tissue regeneration . Importantly, we found that Gel‐CM revealed a higher concentration of growth factors (bFGF, TGF‐β, and EGF) than the Adi‐CM, indicating a higher cell potency in ECM/SVF‐gel.…”

Section: Discussionmentioning

confidence: 74%

Extracellular matrix/stromal vascular fraction gel conditioned medium accelerates wound healing in a murine model

Deng

Feng

et al. 2017

Wound Repair Regeneration

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Conditioned medium (CM) is a new treatment modality in regenerative medicine and has shown a successful outcome in wound healing. We recently introduced extracellular matrix/stromal vascular fraction gel (ECM/SVF-gel), an adipose-derived stem cell and adipose native extracellular matrix-enriched product for cytotherapy. This study aimed to evaluate the effect of CM from ECM/SVF-gel (Gel-CM) on wound healing compared with the conventional CM from adipose tissue (Adi-CM) and stem cell (SVF-CM). In vitro wound healing effect of three CMs on keratinocytes and fibroblasts was evaluated in terms of proliferation property, migratory property, and extracellular matrix production. In vivo, two full-thickness wounds were created on the back of each mice. The wounds were randomly divided to receive Gel-CM, Adi-CM, SVF-CM, and PBS injection. Histologic observations and collagen content of wound skin were made. Growth factors concentration in three CMs was further quantified. In vitro, Gel-CM promoted the proliferation and migration of keratinocytes and fibroblasts and enhanced collagen I synthesis in fibroblasts compared to Adi-CM and SVF-CM. In vivo, wound closure was faster, and dermal and epidermal regeneration was improved in the Gel-CM-treated mice compared to that in Adi-CM and SVF-CM-treated mice. Moreover, The growth factors concentration (i.e., vascular endothelial growth factor, basic fibroblast growth factor, hepatocyte growth factor, and transforming growth factor-β) in Gel-CM were significantly higher than those in Adi-CM and SVF-CM. Gel-CM generated under serum free conditions significantly enhanced wound healing effect compared to Adi-CM and SVF-CM by accelerating cell proliferation, migration, and production of ECM. This improved trophic effect may be attributed to the higher growth factors concentration in Gel-CM. Gel-CM shows potential as a novel and promising alternative to skin wound healing treatment. But limitations include the safety and immunogenicity studies of Gel-CM still remain to be clearly clarified and more data on mechanism study are needed.

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Section: Discussionmentioning

confidence: 74%

Extracellular matrix/stromal vascular fraction gel conditioned medium accelerates wound healing in a murine model

Deng

Feng

et al. 2017

Wound Repair Regeneration

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“…The enhanced expression of angiogenic factors in ECM/SVF-gel and a rich vascular network around the wound area indicated that the faster healing of wounds in the ECM/SVF-gel group could be attributed to increased vascularization. Vascularization of the wound bed is known to play a significant role in the wound healing process [ 13 , 14 ]. Recent cytotherapy strategies using MSCs have aimed at increasing vascularization to achieve wound healing due to their paracrine effects and differentiating potential [ 15 , 16 ].…”

Section: Discussionmentioning

confidence: 99%

“…ECM/SVF-gel extract was prepared according to the manufacturer's instructions [ 13 ]. Briefly, in vivo ECM/SVF-gel samples from all 5 time points were harvested.…”

Section: Methodsmentioning

confidence: 99%

Adipose Extracellular Matrix/Stromal Vascular Fraction Gel Secretes Angiogenic Factors and Enhances Skin Wound Healing in a Murine Model

Sun

Zhou

et al. 2017

BioMed Research International

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Mesenchymal stem cells are an attractive cell type for cytotherapy in wound healing. The authors recently developed a novel, adipose-tissue-derived, injectable extracellular matrix/stromal vascular fraction gel (ECM/SVF-gel) for stem cell therapy. This study was designed to assess the therapeutic effects of ECM/SVF-gel on wound healing and potential mechanisms. ECM/SVF-gel was prepared for use in nude mouse excisional wound healing model. An SVF cell suspension and phosphate-buffered saline injection served as the control. The expression levels of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and monocyte chemotactic protein-1 (MCP-1) in ECM/SVF-gel were analyzed at different time points. Angiogenesis (tube formation) assays of ECM/SVF-gel extracts were evaluated, and vessels density in skin was determined. The ECM/SVF-gel extract promoted tube formation in vitro and increased the expression of the angiogenic factors VEGF and bFGF compared with those in the control. The expression of the inflammatory chemoattractant MCP-1 was high in ECM/SVF-gel at the early stage and decreased sharply during the late stage of wound healing. The potent angiogenic effects exerted by ECM/SVF-gel may contribute to the improvement of wound healing, and these effects could be related to the enhanced inflammatory response in ECM/SVF-gel during the early stage of wound healing.

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“…b). Lu et al . also demonstrated that the application of ATE can significantly improve the regeneration of adipose tissue in an engineering chamber.…”

Section: Therapeutic Applications Of Conditioned Mediummentioning

confidence: 98%

“…Plenty of the recent researches have demonstrated the beneficial effect of the conditioned medium from adipose tissue in angiogenesis and adipogenesis in vitro and in vivo . Besides, conditioned medium from adipose tissue was illustrated to be involved in the vast amount of pathophysiological processes such as wound healing, immunoregulation and regeneration . The vast amount of processes that the conditioned medium from adipose tissue are involved in and the versatility of manner in which they can influence the target receptors make this bioactive mixture an interesting source for both therapeutic and diagnostic applications.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic applications of conditioned medium from adipose tissue

Dai

Zhang

et al. 2016

Cell Proliferation

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For the past number of decades, adipose tissue has attracted significant interest due to its complicated composition and versatile functions. Adipose tissue is no longer considered to be just an energy-storing fat pad, but is also a key ring player in interaction networks between various organs and tissues. A wide range of factors released by adipose tissue are responsible for regulation of adipose tissue and other distant target tissues and cells, such as kidneys, skeletal muscle, the cardiovascular system and the immune system, in an auto-/paracrine manner. A mixture of bioactive molecules makes up the conditioned medium of adipose tissue. The beneficial role played by these bioactive molecules in angiogenesis, wound healing, tissue regeneration and immunomodulation has been demonstrated by various studies. Study of this conditioned medium helps deepen our understanding of underlying mechanisms and broadens the potential for therapeutic applications. In this review, we have aimed to improve fundamental understanding of conditioned medium from adipose tissue and to summarize recent efforts to study its therapeutic applications.

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Adipose tissue extract promotes adipose tissue regeneration in an adipose tissue engineering chamber model

Cited by 16 publications

References 20 publications

Extracellular matrix/stromal vascular fraction gel conditioned medium accelerates wound healing in a murine model

Extracellular matrix/stromal vascular fraction gel conditioned medium accelerates wound healing in a murine model

Adipose Extracellular Matrix/Stromal Vascular Fraction Gel Secretes Angiogenic Factors and Enhances Skin Wound Healing in a Murine Model

Therapeutic applications of conditioned medium from adipose tissue

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