Adipose Tissue as a Dedicated Reservoir of Functional Mast Cell Progenitors

Abstract

White adipose tissue (WAT) is a heterogeneous tissue, found in various locations throughout the body, containing mature adipocytes and the stroma‐vascular fraction (SVF). The SVF includes a large proportion of immune hematopoietic cells, among which, mast cells that contribute to diet‐induced obesity. In this study, we asked whether mast cells present in mice adipose tissue could derive from hematopoietic stem/progenitor cells (HSPC) identified in the tissue. We therefore performed both in vitro and in vivo experiments dedicated to monitoring the progeny of WAT‐derived HSPC. The entire study was conducted in parallel with bone marrow‐derived cells, considered the gold standard for hematopoietic‐lineage studies. Here, we demonstrate that adipose‐derived HSPC contain a precursor‐cell population committed to the mast cell lineage, and able to efficiently home to peripheral organs such as intestine and skin, where it acquires properties of functional tissue mast cells. Additionally, WAT contains a significant mast cell progenitor population, suggesting that the entire mast cell lineage process take place in WAT. Considering the quantitative importance of WAT in the adult organism and the increasing roles recently assigned to mast cells in physiopathology, WAT may represent an important source of mast cells in physiological and pathological situations. STEM CELLS 2010;28:2065–2072

Characterization of Human Adipose Tissue-Resident Hematopoietic Cell Populations Reveals a Novel Macrophage Subpopulation with CD34 Expression and Mesenchymal Multipotency

“…In fact, 1 report demonstrated that murine adipose-derived hematopoietic stem/ progenitor cells contain precursors committed to the mast cell lineage [17]. Another report demonstrated the existence of hemangioblasts in human AT that exhibit the capacity for hematopoietic and endothelial differentiation in vitro [18].…”

Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues

“…First, a thorough digestion and disperse is required to obtain purified adipocytes. Fat tissue has complex cellular composition, including adipocytes, ASCs, preadipocyte, fibroblasts, blood cells, endothelial cells, pericytes, smooth muscle cells and so on [3,9,25,[27][28][29][30][31][32]. The purity of derived DFAT cells might be compromised by the contamination of those cells above when they're attached to the floating adipocytes after incomplete enzymatic treatment.…”

Factores comunes e inductores de inflamación en asma y obesidad

“…Los precursores hematopoyéticos del tejido adiposo pueden dar origen a mastocitos, 69 mismos que, además de participar como células fagocí-ticas, tienen un papel esencial en la inflamación. Estas células progenitoras se han propuesto como las posibles causantes de las diferencias entre la hiperreactividad bronquial en modelos animales obesos y no obesos.…”