Adipose Stem Cell-Based Treatments for Wound Healing

Zeng, Ning; Chen, Hongbo; Wu, Yiping; Liu, Zeming

doi:10.3389/fcell.2021.821652

Cited by 12 publications

(7 citation statements)

References 66 publications

(76 reference statements)

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“…On the other hand, seed cells and corresponding cytokines also play essential roles in tissue repair. ADSCs are widely used in tissue repair because they are easily accessible and can secrete various cytokines [ 12 , 13 ]. Targeted modification of ADSCs can promote their secretion of more cytokines and thus stimulate tissue repair.…”

Section: Introductionmentioning

confidence: 99%

Clinical application of a double-modified sulfated bacterial cellulose scaffold material loaded with FGFR2-modified adipose-derived stem cells in urethral reconstruction

Zhu

Yang

et al. 2022

Stem Cell Res Ther

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Background Urethral stricture and reconstruction are one of the thorny difficult problems in the field of urology. The continuous development of tissue engineering and biomaterials has given new therapeutic thinking to this problem. Bacterial cellulose (BC) is an excellent biomaterial due to its accessibility and strong plasticity. Moreover, adipose-derived stem cells (ADSCs) could enhance their wound healing ability through directional modification. Methods First, we used physical drilling and sulfonation in this study to make BC more conducive to cell attachment and degradation. We tested the relevant mechanical properties of these materials. After that, we attached Fibroblast Growth Factor Receptor 2 (FGFR2)-modified ADSCs to the material to construct a urethra for tissue engineering. Afterward, we verified this finding in the male New Zealand rabbit model and carried out immunohistochemical and imaging examinations 1 and 3 months after the operation. At the same time, we detected the potential biological function of FGFR2 by bioinformatics and a cytokine chip. Results The results show that the composite has excellent repairability and that this ability is correlated with angiogenesis. The new composite in this study provides new insight and therapeutic methods for urethral reconstruction. The preliminary mechanism showed that FGFR2 could promote angiogenesis and tissue repair by promoting the secretion of Vascular Endothelial Growth Factor A (VEGFA) from ADSCs. Conclusions Double-modified sulfonated bacterial cellulose scaffolds combined with FGFR2-modified ADSCs provide new sight and treatments for patients with urethral strictures.

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Section: Introductionmentioning

confidence: 99%

Clinical application of a double-modified sulfated bacterial cellulose scaffold material loaded with FGFR2-modified adipose-derived stem cells in urethral reconstruction

Zhu

Yang

et al. 2022

Stem Cell Res Ther

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“…Taken together with the confocal microscopy analysis results in Figure 5 , ASCs were found to proliferate and survive better while preserving their phenotype in PGH. Furthermore, all target genes associated with skin regeneration were upregulated in PGH, which may increase the growth factor secretion by ASCs to collectively enhance their wound healing functions ( Figure 6 ) [ 32 ]. The mechanisms of wound healing associated with ASCs are complex and diverse and they may participate in the entire wound healing process, including inflammation, proliferation, and remodeling [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

PLGA/Gelatin/Hyaluronic Acid Fibrous Membrane Scaffold for Therapeutic Delivery of Adipose-Derived Stem Cells to Promote Wound Healing

et al. 2022

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Hyaluronic acid (HA) has been suggested to be a preferential material for the delivery of adipose-derived stem cells (ASCs) in wound healing. By incorporating HA in electrospun poly (lactide-co-glycolide) (PLGA)/gelatin (PG) fibrous membrane scaffolds (FMS), we aim to fabricate PLGA/gelatin/HA (PGH) FMS to provide a milieu for 3D culture and delivery of ASCs. The prepared FMS shows adequate cytocompatibility and is suitable for attachment and growth of ASCs. Compared with PG, the PGH offers an enhanced proliferation rate of ASCs, shows higher cell viability, and better maintains an ASC-like phenotype during in vitro cell culture. The ASCs in PGH also show upregulated expression of genes associated with angiogenesis and wound healing. From a rat full-thickness wound healing model, a wound treated with PGH/ASCs can accelerate the wound closure rate compared with wounds treated with PGH, alginate wound dressing, and gauze. From H&E and Masson’s trichrome staining, the PGH/ASC treatment can promote wound healing by increasing the epithelialization rate and forming well-organized dermis. This is supported by immunohistochemical staining of macrophages and α-smooth muscle actin, where early recruitment of macrophages, macrophage polarization, and angiogenesis was found due to the delivered ASCs. The content of type III collagen is also higher than type I collagen within the newly formed skin tissue, implying scarless wound healing. Taken together, using PGH FMS as a topical wound dressing material for the therapeutic delivery of ASCs, a wound treated with PGH/ASCs was shown to accelerate wound healing significantly in rats, through modulating immunoreaction, promoting angiogenesis, and reducing scar formation at the wound sites.

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“…Dermal substitutes, which either replace dermis or epidermis, promote wound healing [9,10]. Stem cell therapy has been used to treat a wide range of skin injuries [2,11,12]. The use of multipotent adult stem cells for the treatment of cutaneous wounds is an attractive option, as these cells can differentiate into multiple cell types.…”

Section: Introductionmentioning

confidence: 99%

“…These cells secrete a variety of cytokines and growth factors which are crucial for the process of wound healing. Moreover, bone marrow-derived mesenchymal stem cells (BM-MSCs) are potential candidates for the regeneration of functional tissue following tissue injury [12]. However, little improvement in organ function has been reported due to poor survival of the transplanted cells at the injured site.…”

Section: Introductionmentioning

confidence: 99%

Potential of stem cell seeded three-dimensional scaffold for regeneration of full-thickness skin wounds

et al. 2022

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Hypoxic wounds are tough to heal and are associated with chronicity, causing major healthcare burden. Available treatment options offer only limited success for accelerated and scarless healing. Traditional skin substitutes are widely used to improve wound healing, however, they lack proper vascularization. Mesenchymal stem cells (MSCs) offer improved wound healing; however, their poor retention, survival and adherence at the wound site negatively affect their therapeutic potential. The aim of this study is to enhance skin regeneration in a rat model of full-thickness dermal wound by transplanting genetically modified MSCs seeded on a three-dimensional collagen scaffold. Rat bone marrow MSCs were efficiently incorporated in the acellular collagen scaffold. Skin tissues with transplanted subcutaneous scaffolds were histologically analysed, while angiogenesis was assessed both at gene and protein levels. Our findings demonstrated that three-dimensional collagen scaffolds play a potential role in the survival and adherence of stem cells at the wound site, while modification of MSCs with jagged one gene provides a conducive environment for wound regeneration with improved proliferation, reduced inflammation and enhanced vasculogenesis. The results of this study represent an advanced targeted approach having the potential to be translated in clinical settings for targeted personalized therapy.

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Adipose Stem Cell-Based Treatments for Wound Healing

Cited by 12 publications

References 66 publications

Clinical application of a double-modified sulfated bacterial cellulose scaffold material loaded with FGFR2-modified adipose-derived stem cells in urethral reconstruction

Clinical application of a double-modified sulfated bacterial cellulose scaffold material loaded with FGFR2-modified adipose-derived stem cells in urethral reconstruction

PLGA/Gelatin/Hyaluronic Acid Fibrous Membrane Scaffold for Therapeutic Delivery of Adipose-Derived Stem Cells to Promote Wound Healing

Potential of stem cell seeded three-dimensional scaffold for regeneration of full-thickness skin wounds

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