Adipose-derived stem cells improve full-thickness skin grafts in a rat model

Vidor, Silvana Bellini; Terraciano, Paula Barros; Valente, Fernanda Soldatelli; Rolim, Verônica Machado; Kuhl, Cristiana Palma; Ayres, Laura Silveira; Garcez, Tuane Nerissa Alves; Lemos, Natália Emerim; Kipper, Cristiano Ely; Pizzato, Sabrina Beal; Driemeier, David; Cirne-Lima, Elizabeth Obino; Contesini, Émerson Antônio

doi:10.1016/j.rvsc.2018.03.014

Cited by 17 publications

(11 citation statements)

References 47 publications

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“…Impaired vascularization within engineered tissues remains a reconstructive challenge (Frueh et al, 2018). Therapeutic potential of ASCs in terms of angiogenesis and tissue regeneration due to self‐renewal, multidirectional differentiation ability, easy collection, and weak immunogenicity is highlighted (Vidor et al, 2018). Additionally, studies have also demonstrated that MSCs can promote the proliferation, migration, and collagen secretion of fibroblasts through a paracrine mechanism independently of the promotion of angiogenesis.…”

Section: Discussionmentioning

confidence: 99%

Generation of niche tuned antifibrotic fibroblasts and non‐viral mediated endothelial commitment using adipose stem cells for dermal graft development

et al. 2020

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Cell‐based skin substitute generation has seen considerable development. Combining synthetic scaffolds with biomimetic fibrin does direct both exogenous and endogenous stem cell differentiation, addressing needs for reliable tissue engineering. However, lack of immediate vasculature within implantable grafts remains critical for its sustenance and integration. Multipotency, high proliferation potential, ability to release multiple growth factors (GFs), and autologous availability highlight the use of human adipose derived mesenchymal stem cells (hADMSCs) in tissue‐engineered dermal grafts (TEDG) construction. However, hADMSCs' insufficiency to independently establish angiogenesis within tissue constructs demands improvement of stem cell application for dermal graft survival. Approaches to harness microenvironmentally sensitive paracrine interactions could improve the angiogenic efficiency of hADMSCs within TEDG. This study conceptualized a fibrin‐based niche, to direct hADMSCs toward a nonfibrotic fibroblast commitment and incorporation of bioengineered hADMSCs, specifically releasing potent angiogenic factors within TEDG. Coexistence of tuned fibroblast and endothelial lineage committed cells contributed to well‐regulated extracellular matrix formation and prevascularization. Adequate cell proliferation; sustained transient release of angiogenic GFs till 20 days; directed dermal, endothelial, fibroblast, and vascular smooth muscle cell differentiation; and favored elastin and collagen deposition were achieved in vitro. In conclusion, specific niche composition and employment of bioengineered hADMSCs favor implantable TEDG construction.

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Section: Discussionmentioning

confidence: 99%

Generation of niche tuned antifibrotic fibroblasts and non‐viral mediated endothelial commitment using adipose stem cells for dermal graft development

et al. 2020

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“…These cells were initially obtained by Zuk et al [29], and have favorable characteristics for cell therapy [12, 14, 15, 2, 8, 10, 11]. These advantages were described in the isolation, expansion and characterization of ASCs from equines, humans and rats [6, 13, 30, 8].…”

Section: Discussionmentioning

confidence: 99%

Adipose stem cells in reparative goat mastitis mammary gland

et al. 2019

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Mesenchymal stem cells have been widely used in the treatment of various chronic diseases. The objective of this survey was to evaluate the therapeutic and regenerative potential of stem cells from adipose tissue (ASCs) in the milk production recovery repair of tissue injury in mastitis goats treated with antimicrobial agents prior to cell therapy. After the diagnosis of mastitis and treatment with gentamicin, eight lactating goats were selected for cellular and subsequent therapy, physical-chemical analysis of milk, ultrasonographic and histopathological examinations. The ASCs were taken from the subcutaneous fat of a young goat cultivated in vitro, marked with Qdots-655 and injected in the left mammary gland, being the right mammary gland used as the control. After 30 days the ultrasonographic and histopathological analyzes were repeated and, in the first lactation period, the physical-chemical analysis of the milk was reapeated. Before the cellular therapy, the physical-chemical quality of the milk was compromised and the ultrasonographic and histopathological analysis revealed a chronic inflammatory process and fibrous tissue. The marking of the ASCs with Qdots enabled the tracking, by fluorescence microscopy (BX41-OLYMPUS), in the mammary tissue. In the ASCs therapy, cultures showed high cellularity and characteristics favorable to preclinical studies; with the therapy the physical-chemical parameters of the milk, fat, protein, temperature and pH showed significant differences among the groups; five animals treated with ASCs reconstituted the functionality of the gland and the connective tissue reduced in quantity and inflammatory infiltrate cells. ASCs have potential for the possible regeneration of fibrous mastitis lesions in the mammary gland, however, it would be necessary to increase injection time for the histopathological analysis, since the reconstitution of the glandular acini within the assessed period was not finalized. ASCs can be used to reestablish milk production in goat with chronic mastitis repair mammary lesions, with potential to be a promising clinical alternative for animal rehabilitation for productivity.

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“…The ADSCs are able to protect axial flaps from the IRI with results as satisfactory as the ones obtained in procedures without ischemia, because of the improvement in the angiogenic response and the blood perfusion elevation [10]. Many studies use the ADSCs to prevent ischemic injuries in skin flaps [7][8][9][10][11][12][13][14], as others [15,16] used them to improve the quality of skin grafts.…”

Section: Journal Of Head Neck and Spine Surgerymentioning

confidence: 99%

“…MSCs have a great potential to the tissue repairment and regeneration in reconstructive plastic surgery, preventing ischemic lesions in skin flaps [7][8][9][10][11][12][13][14] and in skin grafts [15,16]. MSCs are capable of reducing severe atrophy, retraction, fibrosis and ulceration skin signals, caused by radiotherapy [17,18].…”

Section: Introductionmentioning

confidence: 99%

Stem Cells to Improve the Wound Healing

Vidor¹,

Contesini²

2018

JHNSS

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The cellular therapy using Mesenchymal Stem Cells, specially its two subtypes -Bone-marrow Mesenchymal Stem Cells and Adipose Derived Stem Cells -can benefit by virtue of the possibility of differentiating in specialized cells that secrete and suppress growing factors and cytokines necessary in the lesion niche. When attracted by the pro-inflammatory sinalization of the lesion, they act using the paracrine signaling, decreasing the inflammation, increasing the angiogenesis and the cell migration and proliferation. The development in the researches regarding the association of the application of MSCs, with reconstructive surgery practices, leads to effective future results that can bring more benefits to the clinic practice of this field. This paper has the objective of briefly reviewing the literature about the usage of MSCs and its subtypes, the ADSCs, to improve the skin cicatrization.

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Adipose-derived stem cells improve full-thickness skin grafts in a rat model

Cited by 17 publications

References 47 publications

Generation of niche tuned antifibrotic fibroblasts and non‐viral mediated endothelial commitment using adipose stem cells for dermal graft development

Generation of niche tuned antifibrotic fibroblasts and non‐viral mediated endothelial commitment using adipose stem cells for dermal graft development

Adipose stem cells in reparative goat mastitis mammary gland

Stem Cells to Improve the Wound Healing

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