Adipose-Derived Stem Cells: Current Applications and Future Directions in the Regeneration of Multiple Tissues

Zhang, Jiaxin; Liu, Yuzhe; Chen, Yutong; Yuan, Lei; Li, He; Wang, Jincheng; Liu, Qiran; Zhang, Yan

doi:10.1155/2020/8810813

Cited by 116 publications

(104 citation statements)

References 188 publications

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“…Recent studies have demonstrated that the effectiveness of ADSCs in regenerative medicine is due to their paracrine effects [ 13 ]. Thus, ADSCs have been identified as new therapeutic agents in biomedicine [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Adipose-derived mesenchymal stromal cell-derived exosomes promote tendon healing by activating both SMAD1/5/9 and SMAD2/3

et al. 2021

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Background The use of adipose-derived mesenchymal stromal cell-derived exosomes (ADSC-Exos) may become a new therapeutic method in biomedicine owing to their important role in regenerative medicine. However, the role of ADSC-Exos in tendon repair has not yet been evaluated. Therefore, we aimed to clarify the healing effects of ADSC-Exos on tendon injury. Methods The adipose-derived mesenchymal stromal cells (ADSCs) and tendon stem cells (TSCs) were isolated from the subcutaneous fat and tendon tissues of Sprague-Dawley rats, respectively, and exosomes were isolated from ADSCs. The proliferation and migration of TSCs induced by ADSC-Exos were analyzed by EdU, cell scratch, and transwell assays. We used western blot to analyze the tenogenic differentiation of TSCs and the role of the SMAD signaling pathways. Then, we explored a new treatment method for tendon injury, combining exosome therapy with local targeting using a biohydrogel. Immunofluorescence and immunohistochemistry were used to detect the expression of inflammatory and tenogenic differentiation after tendon injury, respectively. The quality of tendon healing was evaluated by hematoxylin-eosin (H&E) staining and biomechanical testing. Results ADSC-Exos could be absorbed by TSCs and promoted the proliferation, migration, and tenogenic differentiation of these cells. This effect may have depended on the activation of the SMAD2/3 and SMAD1/5/9 pathways. Furthermore, ADSC-Exos inhibited the early inflammatory reaction and promoted tendon healing in vivo. Conclusions Overall, we demonstrated that ADSC-Exos contributed to tendon regeneration and provided proof of concept of a new approach for treating tendon injuries.

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Section: Introductionmentioning

confidence: 99%

Adipose-derived mesenchymal stromal cell-derived exosomes promote tendon healing by activating both SMAD1/5/9 and SMAD2/3

et al. 2021

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“…Additionally, the expression of CD36 and lack of CD106 distinguish ASCs from bone marrow MSCs [74]. However, there is still a lack of consensus in definitive markers that discriminate ASCs [79], because surface biomarkers differ depending on donor sites and culture procedure [80].…”

Section: Ascs and The Regenerative Property Of Fat Graftingmentioning

confidence: 99%

Lipofilling in Breast Oncological Surgery: A Safe Opportunity or Risk for Cancer Recurrence?

Piccotti

Rybińska

Scoccia

et al. 2021

IJMS

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Lipofilling (LF) is a largely employed technique in reconstructive and esthetic breast surgery. Over the years, it has demonstrated to be extremely useful for treatment of soft tissue defects after demolitive or conservative breast cancer surgery and different procedures have been developed to improve the survival of transplanted fat graft. The regenerative potential of LF is attributed to the multipotent stem cells found in large quantity in adipose tissue. However, a growing body of pre-clinical evidence shows that adipocytes and adipose-derived stromal cells may have pro-tumorigenic potential. Despite no clear indication from clinical studies has demonstrated an increased risk of cancer recurrence upon LF, these observations challenge the oncologic safety of the procedure. This review aims to provide an updated overview of both the clinical and the pre-clinical indications to the suitability and safety of LF in breast oncological surgery. Cellular and molecular players in the crosstalk between adipose tissue and cancer are described, and heterogeneous contradictory results are discussed, highlighting that important issues still remain to be solved to get a clear understanding of LF safety in breast cancer patients.

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“…It is well documented that adipose-derived mesenchymal stem cells (ADSCs) have multilineage differentiation potential and can differentiate into different cell types, including adipocytes, osteoblasts, chondrocytes, and hepatocytes [ 10 ]. ADSCs can also repair damaged organs and tissues through releasing a variety of paracrine factors and extracellular vesicles (EVs) [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Adipose-derived stem cells therapy effectively attenuates PM2.5-induced lung injury

Gao

Yuan

et al. 2021

Stem Cell Res Ther

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Background The adverse health effects of fine particulate matter (PM2.5) exposure are associated with marked inflammatory responses. Adipose-derived stem cells (ADSCs) have immunosuppressive effects, and ADSC transplantation could attenuate pulmonary fibrosis in different animal disease models. However, whether ADSCs affect PM2.5-induced lung injury has not been investigated. Method C57BL/6 mice were exposed to PM2.5 every other day via intratracheal instillation for 4 weeks. After that, the mice received tail vein injections of ADSCs every 2 weeks. Results ADSC transplantation significantly attenuated systemic and pulmonary inflammation, cardiac dysfunction, fibrosis, and cell death in PM2.5-exposed mice. RNA-sequencing results and bioinformatic analysis suggested that the downregulated differentially expressed genes (DEGs) were mainly enriched in inflammatory and immune pathways. Moreover, ADSC transplantation attenuated PM2.5-induced cell apoptosis and pyroptosis in the lungs and hearts. Conclusion ADSCs protect against PM2.5-induced adverse health effects through attenuating pulmonary inflammation and cell death. Our findings suggest that ADSC transplantation may be a potential therapeutic approach for severe air pollution-associated diseases.

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Adipose-Derived Stem Cells: Current Applications and Future Directions in the Regeneration of Multiple Tissues

Cited by 116 publications

References 188 publications

Adipose-derived mesenchymal stromal cell-derived exosomes promote tendon healing by activating both SMAD1/5/9 and SMAD2/3

Adipose-derived mesenchymal stromal cell-derived exosomes promote tendon healing by activating both SMAD1/5/9 and SMAD2/3

Lipofilling in Breast Oncological Surgery: A Safe Opportunity or Risk for Cancer Recurrence?

Adipose-derived stem cells therapy effectively attenuates PM2.5-induced lung injury

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