Adipose-Derived Stem Cells Accelerate Diabetic Wound Healing through the Induction of Autocrine and Paracrine Effects

Kuo, Yur‐Ren; Wang, Chunting; Cheng, Jiin‐Tsuey; Kao, Gour-Shenq; Chiang, Yuan‐Cheng; Wang, Ching‐Jen

doi:10.3727/096368915x687921

Cited by 127 publications

(110 citation statements)

References 39 publications

Supporting

Mentioning

105

Contrasting

Order By: Relevance

“…We hypothesize that the additional benefits observed with betatrophin overexpression may result from the combination of betatrophin and growth factors secreted by MSCs and/or from the immunomodulatory and anti-apoptotic properties of the ADMSCs. The anti-inflammatory capacity of the ADMSCs observed in the present study was consistent with a previous study by Kuo et al (63), which showed that ADMSCs accelerated diabetic wound healing by reducing the pro-inflammatory environment and increasing growth factor expression.…”

Section: Discussionsupporting

confidence: 93%

Transplantation of betatrophin-expressing adipose-derived mesenchymal stem cells induces β-cell proliferation in diabetic mice

Sun

Liu

et al. 2017

International Journal of Molecular Medicine

View full text Add to dashboard Cite

Recent progress in regenerative medicine has suggested that mesenchymal stem cell (MSC)-based therapy is a novel potential cure for diabetes. Betatrophin is a newly identified hormone that can increase the production and expansion of insulin-secreting β-cells when administered to mice. In this study, we evaluated the effect of betatrophin overexpression by human adipose-derived MSCs (ADMSCs) by in vitro experiments, as well as following their transplantation into a mice with streptozotocin (STZ)-induced diabetes. The overexpression of betatrophin did not affect the ADMSCs in terms of proliferation, differentiation and morphology. However, the co-culture of human islets with ADMSCs overexpressing betatrophin (ADMSCs-BET) induced islet proliferation, β-cell specific transcription factor expression, and the islet production of insulin under the stimulation of glucose or KCl and Arg. In addition, ADMSCs-BET enhanced the anti-inflammatory and anti-apoptotic effects of the co-cultured islets compared with ADMSCs cultured alone. In mice with STZ-induced diabetes, the transplantation of ADMSCs-BET ameliorated the hyperglycemia and weight loss associated with STZ-induced diabetes; ADMSCs-BET also significantly enhanced the ratio of β-cells per islet compared to the transplantation of ADMSCs alone. Thus, our study demonstrates a novel strategy for inducing β-cell regeneration. ADMSCs-BET may replace insulin injections by increasing the number of endogenous insulin-producing cells in patients with diabetes. This combined strategy of ADMSC transplantation and gene therapy may prove to be a useful therapy for the treatment of diabetes.

show abstract

Section: Discussionsupporting

confidence: 93%

Transplantation of betatrophin-expressing adipose-derived mesenchymal stem cells induces β-cell proliferation in diabetic mice

Sun

Liu

et al. 2017

International Journal of Molecular Medicine

View full text Add to dashboard Cite

show abstract

“…In contrast with the expected and found in other studies 8,11 , there was no difference in microscopic evaluation, the inflammatory process was not changed. Probably in immunohistochemistry evaluation the results would be more satisfying.…”

Section: Resultscontrasting

confidence: 55%

“…There is a dependent of fibroblast growth factor, autocrine and paracrine secretion of substances as tipe I collagen and neoangiogenesis 8,16 .…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Stem cells from adipose tissue improve the time of wound healing in rats

et al. 2016

View full text Add to dashboard Cite

PURPOSE:To evaluate the Adipose Stem Cells (ACS) therapy efficacy on the time and quality of wound healing process in rats. METHODS:Nine male Wistar rats were randomly distributed into three groups I) 7 days of healing; II) 14 days of healing; III) 21 days of healing. Four incisions were made on the dorsal surface of each rat and then treated with intralesional ACS, meloxicam, and no treatment and ACS+meloxicam. Macroscopic evaluation was measured by percentage of healing and histopathological by hematoxylineosin was performed. RESULTS:All groups have the wound reduced during the three weeks (p<0.001) and after 14 days of healing had greater reduction than others. Wounds treated with ASC had accelerated healing in relation to no treatment and only meloxicam (p<0.001), excepting the ASC+Meloxicam that was similar (p=0.13). There was no difference in histopathological analysis between lesions.CONCLUSION: Adipose stem cell have benefits in reducing time of healing of experimental model of wound in rats, observed 7 days of after application.

show abstract

“…The superficial localization of infection in the wound enables exciting new perspectives by means of in vivo imaging systems in chronic wound science. 63 …”

mentioning

confidence: 99%

Animal models of chronic wound care: the application of biofilms in clinical research

Trøstrup¹,

Thomsen²,

Calum³

et al. 2016

CWCMR

View full text Add to dashboard Cite

Chronic wounds are a substantial clinical problem affecting millions of people worldwide. Pathophysiologically, chronic wounds are stuck in the inflammatory state of healing. The role of bacterial biofilms in suppression and perturbation of host response could be an explanation for this observation. An inhibiting effect of bacterial biofilms on wound healing is gaining significant clinical attention over the last few years. There is still a paucity of suitable animal models to recapitulate human chronic wounds. The etiology of the wound (venous insufficiency, ischemia, diabetes, pressure) has to be taken into consideration as underlying pathophysiological mechanisms and comorbidities display tremendous variation in humans. Confounders such as infection, smoking, chronological age, sex, medication, metabolic disturbances, and renal impairment add to the difficulty in gaining systematic and comparable studies on nonhealing wounds. Relevant hypotheses based on clinical or in vitro observations can be tested in representative animal models, which provide crucial tools to uncover the pathophysiology of cutaneous skin repair in infectious environments. Disposing factors, species of the infectious agent(s), and time of establishment of the infection are well defined in suitable animal models. In addition, several endpoints can be involved for evaluation. Animals do not display chronic wounds in the way that humans do. However, in many cases, animal models can mirror the pathological conditions observed in humans, although discrepancies between human and animal wound repair are obvious. The use of animal models should be refined and replaced whenever possible, and reproducibility and clinical relevance should be strived. This review aimed at giving an overview of the model systems and major findings for inspiration for clinicians and researchers involved in handling chronic nonhealing wounds. Relevant animal models on wound repair are discussed, and our novel wound model on the host/pathogen interplay is presented. In this model, murine wounds are stuck in a polymorphonuclear neutrophil granulocyte-dominated inflammation due to the presence of visually confirmed Pseudomonas aeruginosa biofilm located in the dermis and subcutaneous fatty tissue.

show abstract

Adipose-Derived Stem Cells Accelerate Diabetic Wound Healing through the Induction of Autocrine and Paracrine Effects

Cited by 127 publications

References 39 publications

Transplantation of betatrophin-expressing adipose-derived mesenchymal stem cells induces β-cell proliferation in diabetic mice

Transplantation of betatrophin-expressing adipose-derived mesenchymal stem cells induces β-cell proliferation in diabetic mice

Stem cells from adipose tissue improve the time of wound healing in rats

Animal models of chronic wound care: the application of biofilms in clinical research

Contact Info

Product

Resources

About