Adipose-derived stem cell-conditioned medium protects fibroblasts at different senescent degrees from UVB irradiation damages

Guo, Shu; Wang, Ting; Zhang, Shuangyi; Chen, Peng; Cao, Zi-Jun; Lian, Wenqin; Guo, Jiayan; Kang, Yue

doi:10.1007/s11010-019-03630-8

Cited by 33 publications

(40 citation statements)

References 49 publications

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“…Moreover, depending on culture conditions, adipose stem cells show different effects on wound healing. Thus, compared with that obtained using two- [15,16]; EGF [15]; PDGF-AA [15,17]; VEGF [15] KGF [16]; TGF-β [16]; HGF [16,18], IGF-1 [44]; BDNF [19] ADSC-Exo 1. Add ADSC to basal medium containing 10% fetal bovine serum for cultivation 2.…”

Section: Adsc-cm and Wound Healingmentioning

confidence: 99%

“…It was also reported that the expression of type 1 procollagen was significantly upregulated and that of MMP-1 was significantly downregulated in a coculture of ADSC-CM and UV-irradiated human dermal fibroblasts (HDFs) compared with that in the control group [16]. Additionally, Guo et al [17] explored the protective effect of ADSC-CM on fibroblasts against UVB irradiation. These researchers used HDFs at different degrees of senescence, designating the 5th, 15th, and 28th generations as young, intermediate, and senescent cells, respectively.…”

Section: Adsc-cm and Skin Agingmentioning

confidence: 99%

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Therapeutic applications of adipose cell-free derivatives: a review

et al. 2020

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Background: Adipose-derived stem cells (ADSCs) have become one of the most utilized adult stem cells due to their abundance and accessibility. Recent studies have shown that paracrine cytokines, exosomes, and other active substances are the main factors through which ADSCs exert their biological effects. Main body: Adipose cell-free derivatives have been recently gaining attention as potential therapeutic agents for various human diseases. These derivatives include ADSC-conditioned medium (ADSC-CM), ADSC exosomes (ADSC-Exo), and cell-free adipose tissue extracts (ATEs), all of which can be conveniently carried, stored, and transported. Currently, research on ADSC-conditioned medium (ADSC-CM) and ADSC exosomes (ADSC-Exo) is surging. Moreover, cell-free adipose tissue extracts (ATEs), obtained by purely physical methods, have emerged as the focus of research in recent years. Conclusion: Adipose cell-free derivatives delivery can promote cell proliferation, migration, and angiogenesis, suppress cell apoptosis, and inflammation, as well as reduce oxidative stress and immune regulation. Thus, adipose cell-free derivatives have a broad therapeutic potential in many areas, as they possess anti-skin aging properties, promote wound healing, reduce scar formation, and provide myocardial protection and neuroprotection. This article summarizes these effects and reviews research progress in the use of adipose cell-free derivatives.

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Section: Adsc-cm and Wound Healingmentioning

confidence: 99%

Section: Adsc-cm and Skin Agingmentioning

confidence: 99%

Therapeutic applications of adipose cell-free derivatives: a review

et al. 2020

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“…The major inducer of the cell cycle arrest is p21, which is downregulated by ADSCs in fibroblasts [ 34 ]. ADSC-CM treatment decreased cellular senescence induced by UVB and SA- β -Gal in HDFs [ 92 ], which can be explained by the reduced oxidative stress and attenuated DNA damage based on the oxidative stress theory. Besides, overexpression of VEGF in ADSCs promoted the function of ADSCs on downregulating SA- β -Gal and inhibiting senescence in fibroblasts injured by UVR [ 34 ].…”

Section: Adscs Mechanisms In Photoagingmentioning

confidence: 99%

“…The molecular interaction between fibroblasts and the ECM is obstructed due to the decrease of collagen, which eventually results in the damage of fibroblast function and further collagen decrease [ 94 ]. It is reported that ADSC-Exo can promote the migration ability of HDFs irradiated by UVB [ 95 ], suppress the overexpression of MMP-1 [ 95 , 96 ], MMP-2 [ 95 , 97 ], MMP-3 [ 95 , 96 ], MMP-9 [ 92 , 95 ] and MMP-13 [ 97 ] caused by UVB. Moreover, it can increase collagen I, II, III, and V and elastin expression [ 92 , 95 ].…”

Section: Adscs Mechanisms In Photoagingmentioning

confidence: 99%

“…It is reported that ADSC-Exo can promote the migration ability of HDFs irradiated by UVB [ 95 ], suppress the overexpression of MMP-1 [ 95 , 96 ], MMP-2 [ 95 , 97 ], MMP-3 [ 95 , 96 ], MMP-9 [ 92 , 95 ] and MMP-13 [ 97 ] caused by UVB. Moreover, it can increase collagen I, II, III, and V and elastin expression [ 92 , 95 ]. Inhibitor of metalloproteinase (TIMP)-1 and TGF- β 1 that are critical factors contributing to suppressing MMP and synthesizing ECM were upregulated in extracellular vesicles from ADSCs (ADSC-EV)-treated HDFs after UVB exposure [ 95 ].…”

Section: Adscs Mechanisms In Photoagingmentioning

confidence: 99%

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Application of adipose-derived stem cells in photoaging: basic science and literature review

Chen

2020

Stem Cell Res Ther

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Photoaging is mainly induced by continuous exposure to sun light, causing multiple unwanted skin characters and accelerating skin aging. Adipose-derived stem cells(ADSCs) are promising in supporting skin repair because of their significant antioxidant capacity and strong proliferation, differentiation, and migration ability, as well as their enriched secretome containing various growth factors and cytokines. The identification of the mechanisms by which ADSCs perform these functions for photoaging has great potential to explore therapeutic applications and combat skin aging. We also review the basic mechanisms of UV-induced skin aging and recent improvement in pre-clinical applications of ADSCs associated with photoaging. Results showed that ADSCs are potential to address photoaging problem and might treat skin cancer. Compared with ADSCs alone, the secretome-based approaches and different preconditionings of ADSCs are more promising to overcome the current limitations and enhance the anti-photoaging capacity.

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Combination therapy of iPSC‐derived conditioned medium with ceftriaxone alleviates bacteria‐induced lung injury by targeting the NLRP3 inflammasome

Wann

Ling

Huang

et al. 2021

Journal Cellular Physiology

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The lung is the first and most frequent organ to fail among sepsis patients. The mortality rate of sepsis-related acute lung injury (ALI) is high. Despite appropriate antimicrobial therapy, no treatment strategies are available for sepsis-induced ALI. Stem cell-mediated paracrine signaling is a potential treatment method for various diseases. This study aimed to examine the effects of induced pluripotent stem cell-derived conditioned medium (iPSC-CM) combined with antibiotics on ALI in a rat model of Escherichia coli-induced sepsis. Rats were administered either iPSC-CM or the vehicle (saline) with antibiotics (ceftriaxone). After 72 h, liquid biopsy, bronchoalveolar lavage fluid (BALF), and tissues were harvested for analysis. Survival rates were observed for up to 3 days. Furthermore, we examined the effects of iPSC-CM on cytokine production, metalloproteinase 9 (MMP-9) expression, and NLRP3-ASC interaction in RAW264.7 cells stimulated with lipopolysaccharide/interferon-γ (LPS/IFN-γ). Combined treatment of iPSC-CM with antibiotics significantly improved survival in E. coli-infected rats (p = 0.0006). iPSC-CM ameliorated E. coli-induced infiltration of macrophages, reducing the number of cells in BALF, and suppressing interleukin (IL)-1β, MIP-2, IL-6, and MMP-9 messenger RNA in lung sections. iPSC-CM treatment attenuated NLRP3 expression and inhibited NLRP3 inflammasome activation by disrupting NLRP3-mediated ASC complex formation in LPS/IFN-γ-primed RAW264.7 cells. This study reveals the mechanisms underlying iPSC-CMconferred anti-inflammatory activity in ALI through the attenuation of macrophage recruitment to the lung, thus inactivating NLRP3 inflammasomes in macrophages. iPSC-CM therapy may be a useful adjuvant treatment to reduce sepsis-related mortality by ameliorating ALI.

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Adipose-derived stem cell-conditioned medium protects fibroblasts at different senescent degrees from UVB irradiation damages

Cited by 33 publications

References 49 publications

Therapeutic applications of adipose cell-free derivatives: a review

Therapeutic applications of adipose cell-free derivatives: a review

Application of adipose-derived stem cells in photoaging: basic science and literature review

Combination therapy of iPSC‐derived conditioned medium with ceftriaxone alleviates bacteria‐induced lung injury by targeting the NLRP3 inflammasome

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