Adipose-derived mesenchymal stromal cells induce immunomodulatory macrophages which protect from experimental colitis and sepsis

Anderson, Per; Souza-Moreira, Luciana; Morell, María; Caro, Marta; O’Valle, Francisco; González‐Rey, Elena; Delgado, Mario

doi:10.1136/gutjnl-2012-302152

Cited by 185 publications

(177 citation statements)

References 38 publications

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“…In our present study, we were able to reproduce our results with the 5 dose protocol of The mechanisms of action hypothesized for MSCs anti-inflammatory properties have been investigated in many studies [55][56][57][58][59][60]. aMSCs showed enhanced anti-inflammatory IL-10 secretion [73] together with decreased pro-inflammatory TNF-α, INF-γ, IL-1β and their downstream effects on various elements of the immune system.…”

Section: Discussionsupporting

confidence: 74%

“…Regarding the mechanism of action of MSCs, as stated earlier, researchers are suggesting that, the therapeutic benefits of MSCs in RORM might be mediated by their paracrine effects with or without homing to the injury site, rather than their differentiation properties replacing the damaged cells [55,70,71,195,196].…”

Section: Chapter 7 Discussionmentioning

confidence: 99%

“…CD11b and CD45) [74][75][76]. In addition to their multilineage differentiation potential, aMSCs have anti-inflammatory/immune-modulatory and paracrine effects [55][56][57][58][59][60]. They also have the ability to home to the site of tissue injury after irradiation and inflammation [74,77,78].…”

Section: Methodsmentioning

confidence: 99%

“…MSCs are multipotent progenitor cells that have multi-lineage differentiation potential and recently identified anti-inflammatory and immunomodulatory properties that have been applied in numerous current preclinical and clinical studies [55][56][57][58][59][60][61][62][63][64][65][66][67][68][69][70].…”

Section: Mesenchymal Stromal/stem Cells Therapymentioning

confidence: 99%

See 3 more Smart Citations

Adipose mesenchymal stromal cells minimize and repair radiation-induced oral mucositis

et al. 2016

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Section: Discussionsupporting

confidence: 74%

Section: Chapter 7 Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Mesenchymal Stromal/stem Cells Therapymentioning

confidence: 99%

See 2 more Smart Citations

Adipose mesenchymal stromal cells minimize and repair radiation-induced oral mucositis

et al. 2016

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“…MSCs are stromal cells that can be harvested from bone marrow, adipose tissue, and umbilical cords [26][27][28]. These cells have been regarded as prospective therapeutic tools because of their characteristics, such as multipotent differentiation, self-renewal, immunomodulation, and immunoprivilege [29].…”

Section: Discussionmentioning

confidence: 99%

Autophagy Improves the Immunosuppression of CD4+ T Cells by Mesenchymal Stem Cells Through Transforming Growth Factor-β1

Gao

Cen

Wang

et al. 2016

Stem Cells Translational Medicine

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Mesenchymal stem cells (MSCs) have been extensively investigated as a promising approach to treat many autoimmune and inflammatory diseases. The stress condition would affect the therapeutic efficacy and induce autophagy of MSCs. However, whether autophagy would affect the immunosuppressive capacity of MSCs is largely unknown. The present study aimed to assess whether autophagy plays an important role in regulating the immunomodulation of MSCs and the undermechanisms. We successfully inhibited and induced autophagy of MSCs using 3-methyladenine (3-MA) and rapamycin, respectively. Our results demonstrated that rapamycin strengthened the capacity of MSCs to inhibit CD4 + T-cell proliferation, whereas 3-MA weakened the inhibitory ability of MSCs. Mechanistically, 3-MA-pretreated MSCs secreted less, whereas rapamycin-pretreated MSCs secreted more transforming growth factor-b1 (TGF-b1) compared with the control cells. Furthermore, exogenous TGF-b1 addition recovered the immunosuppressive capacity of 3-MA-pretreated MSCs, whereas exogenous anti-TGF-b1 antibody addition reduced the immunosuppressive capacity of rapamycin-pretreated MSCs. These results indicated that the autophagy level regulates the immunosuppression of CD4 + T cells by MSCs through affecting TGF-b1 secretion and provides a novel method for improving the therapeutic efficacy of MSCs by activating autophagy. STEM CELLS TRANSLATIONAL MEDICINE 2016;5:1496-1505 SIGNIFICANCEMesenchymal stem cell (MSC)-based therapy is a promising tool to treat many diseases. Autophagy occurred in MSCs during their application, especially in those exposed to stress conditions. However, whether autophagy will affect the therapeutic efficacy of MSCs is largely unknown. This study makes a significant contribution to demonstrate that autophagy could improve the immunosuppression of CD4 + T cells by mesenchymal stem cells through transforming growth factor-b1. Therefore, regulation of autophagy in MSCs would provide a promising strategy to improve the therapeutic efficacy of these cells.

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The role of mesenchymal stromal cells in the treatment of ulcerative colitis andCrohn's disease

Gregoire

Lechanteur

Briquet

et al. 2016

The Biology and Therapeutic Application of Mesenchymal Cells

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Differentiation and stimulation of tissue repair, 740 50.6 Mesenchymal stromal cell administration in inflammatory bowel diseases, 740 50.6.1 Mesenchymal stromal cell administration for fistulizing Crohn's disease, 741 50.6.2 Autologous mesenchymal stromal cell administration for fistulizing Crohn's disease, 741 50.1 Inflammatory bowel diseases Inflammatory bowel diseases (IBDs) are chronic relapsing dis eases associated with debilitating symptoms and leading to progressive intestinal tissue damage and disability (Figure 50.1). Crohn's disease (CD) can affect any part of the gastrointestinal tract, and lesions are often segmental and transmural. The disease was named after gastroenterologist Dr Burrill Bernard

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Adipose-derived mesenchymal stromal cells induce immunomodulatory macrophages which protect from experimental colitis and sepsis

Abstract: ASCs induce a distinct regulatory activation state of macrophages which possess potent immunomodulatory ability and therapeutic potential in inflammatory bowel diseases and sepsis.

Cited by 185 publications

References 38 publications

Adipose mesenchymal stromal cells minimize and repair radiation-induced oral mucositis

Adipose mesenchymal stromal cells minimize and repair radiation-induced oral mucositis

Autophagy Improves the Immunosuppression of CD4+ T Cells by Mesenchymal Stem Cells Through Transforming Growth Factor-β1

The role of mesenchymal stromal cells in the treatment of ulcerative colitis andCrohn's disease

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