Adiponectin: Stability in Plasma over 36 Hours and Within-Person Variation over 1 Year

Pischon, Tobias; Hotamışlıgil, Gökhan S.; Rimm, Eric B.

doi:10.1373/49.4.650

Cited by 139 publications

(117 citation statements)

References 30 publications

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“…Serum adiponectin values were measured on a single sample, which may have limited associations; however, adiponectin levels show minimal diurnal variation, are stable during the morning hours when these samples were collected and single measurements have been shown to accurately reflect levels over a 1-year period. [50][51][52][53] It is not known whether long-term storage effects adiponectin levels; in this cohort, adiponectin concentrations were not significantly related to storage time, and levels were similar to published values for individuals of comparable age and adiposity. 3,5 Endogenous sex hormones and adiponectin GA Laughlin et al Adiponectin circulates in complex structures, predominantly a low-and a high-molecular weight (HMW) form.…”

Section: Discussionsupporting

confidence: 74%

Sex-specific determinants of serum adiponectin in older adults: the role of endogenous sex hormones

2006

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Objective: To assess the sex-specific association of adiponectin with multiple factors thought to influence its levels, with a special emphasis on endogenous sex hormones. Design and methods: A cross-sectional study of determinants of serum adiponectin in 873 men and 673 postmenopausal women, ages 50-92. Factors evaluated include age, body size, fat distribution, lifestyle (exercise, smoking, alcohol intake), insulin resistance, renal function and endogenous sex hormone levels (total and bioavailable testosterone and estradiol). Results: Median serum adiponectin was 50% higher in women than men (Po0.001). In unadjusted analyses, adiponectin was positively related to age, alcohol intake, high-density lipoprotein (HDL) and testosterone, and negatively related to waist girth, body mass index, Homeostasis Model Assessment for Insulin Resistance (HOMA-IR), triglycerides and bioavailable estradiol in both sexes (all Po0.01). Adiponectin was positively related to blood urea nitrogen, a measure of renal function, in men only (Po0.001). Sex-specific multivariate linear regressions adjusting for HDL and triglycerides showed that only age, HOMA-IR and sex hormones independently predicted circulating adiponectin for both men and women. Higher levels of endogenous testosterone and lower bioavailable estradiol concentrations each predicted higher adiponectin; this was true for both sexes, and was not explained by differences in age, adiposity, alcohol intake, insulin resistance or lipoprotein levels. Conclusions: The association of adiponectin with the factors studied here is strikingly similar for men and women. Sex differences in circulating adiponectin levels in older adults cannot be explained by sex hormone regulation.

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Section: Discussionsupporting

confidence: 74%

Sex-specific determinants of serum adiponectin in older adults: the role of endogenous sex hormones

2006

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“…However we note the harsher forces that samples processed by sucrose gradient velocity sedimentation are subject to compared to the relatively mild conditions of an ELISA. That we observed no decrease in total plasma adiponectin levels after delayed processing of blood samples appears consistent with a report where total plasma adiponectin was unaffected following storage of blood samples as described by Pischon and colleagues (Pischon et al, 2003). In contrast Pischon and colleagues showed EDTA plasma samples led to an increase in total adiponectin levels of almost 20% (p=0.05), in contrast to plasma samples collected in sodium-heparin, prompting the authors to suggest that the apparent increase in the presence of EDTA may reflect dissociation of the HMW adiponectin multimers resulting in enhanced detection of lower order multimers by the antibodies in the ELISA (Pischon et al, 2003).…”

Section: Discussionsupporting

confidence: 94%

“…That we observed no decrease in total plasma adiponectin levels after delayed processing of blood samples appears consistent with a report where total plasma adiponectin was unaffected following storage of blood samples as described by Pischon and colleagues (Pischon et al, 2003). In contrast Pischon and colleagues showed EDTA plasma samples led to an increase in total adiponectin levels of almost 20% (p=0.05), in contrast to plasma samples collected in sodium-heparin, prompting the authors to suggest that the apparent increase in the presence of EDTA may reflect dissociation of the HMW adiponectin multimers resulting in enhanced detection of lower order multimers by the antibodies in the ELISA (Pischon et al, 2003). Collectively then, stability of the HMW multimers appears, at least in this small study, to be dependent on a variety of complementary factors including the degree of PTM and presence of calcium (Hickman & Whitehead, 2012;Richards et al, 2006;Simpson & Whitehead, 2010;Wang et al, 2006).…”

Section: Discussionsupporting

confidence: 94%

“…Such studies show that both total and HMW adiponectin are stable in serum or plasma when subjected to multiple freeze-thaw cycles (Sattar et al, 2008;Schraw et al, 2008;Suominen, 2004). Furthermore, total adiponectin has been reported to be stable in blood for up to 36 h, in Styrofoam containers containing ice packs, prior to processing for measurement in plasma (Pischon et al, 2003). In this study we extended the pre-analytical storage conditions, which is more in line with what occurs in remote collection centres using EDTA plasma and serum samples for total adiponectin as well as the more metabolically active HMW multimers.…”

Section: Discussionmentioning

confidence: 99%

“…However, the effect of pre-processing conditions such as the temperature and length of time that a blood sample may be stored and transported prior to centrifugation, separating serum or plasma from cells and subsequent storage of serum or plasma is still unclear. Total adiponectin has been shown to be stable for up to 36 h in Styrofoam containers containing ice packs prior to centrifugation in EDTA and sodium heparin samples (Pischon, Hotamisligil, & Rimm, 2003). However, the effects of processing delays and prolonged blood sample storage on HMW adiponectin specifically have not been investigated to our knowledge.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Delayed Sample Processing on Determination of Total and High Molecular Weight (HMW) Adiponectin in Serum and Plasma: A Pilot Study

Ng¹,

Rose²,

Keshvari³

et al. 2016

IJC

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Adiponectin is a beneficial adipocyte-secreted hormone, which circulates in a variety of multimeric forms termed low and high molecular weight (LMW/HMW). Effectiveness of clinical therapeutic trials which target adiponectin rely on accurate determination of circulating total and HMW adiponectin levels but the accuracy may be influenced by variations in sample handling processes. The aim of this pilot study was to investigate the effects of delayed processing of blood samples on the concentration of total and HMW adiponectin. Materials and Methods: Fasting blood samples were collected for analysis of total and HMW adiponectin concentrations in EDTA plasma and serum from eight healthy participants. Samples were centrifuged post 15 min storage at 4 o C as the comparative "ideal" method or after up to 72 h of refrigerated storage or 6 h at room temperature. Total and HMW adiponectin concentrations were measured by ELISA.Results: Under ideal handling conditions measurements of total and HMW adiponectin concentrations were significantly higher in serum than in plasma (mean difference: -1.3 µg/mL [95% CI: -1.6, -1.0], p<0.001; and, -0.6 µg/mL [95% CI: -0.7, -0.5], p<0.001, respectively). Storage of blood samples at 4 o C for 72 h resulted in significant reductions in concentration of total adiponectin in serum (mean difference: -1.4 µg/mL [95% CI: -2.0, -0.8], p=0.001) and HMW adiponectin in plasma (mean difference: -0.6 µg/mL [95%CI: -0.9, -0.2], p=0.007), compared with ideal conditions. Further analysis of serum samples showed a significant decrease in total adiponectin concentration after 6 h storage at 4 o C (mean difference: -1.4 µg/mL [95% CI: -2.0, -0.8], p=0.001) compared with ideal conditions. Conclusions: Delayed processing of samples may have differential effects on the concentration of total and HMW adiponectin in serum or plasma. Larger studies are warranted for clinical intervention trials.

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Plasma Adiponectin Levels and Risk of Myocardial Infarction in Men

Pischon¹

2004

JAMA

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1,550

557

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Context Adiponectin, a recently discovered adipocyte-derived peptide, is involved in the regulation of insulin sensitivity and lipid oxidation and, purportedly, in the development of atherosclerosis and coronary heart disease in humans. Objective To assess prospectively whether plasma adiponectin concentrations are associated with risk of myocardial infarction (MI). Design, Setting, and Participants Nested case-control study among 18225 male participants of the Health Professionals Follow-up Study aged 40 to 75 years who were free of diagnosed cardiovascular disease at the time of blood draw (1993-1995). During 6 years of follow-up through January 31, 2000, 266 men subsequently developed nonfatal MI or fatal coronary heart disease. Using risk set sampling, controls were selected in a 2:1 ratio matched for age, date of blood draw, and smoking status (n=532). Main Outcome Measure Incidence of nonfatal MI and fatal coronary heart disease by adiponectin level. Results After adjustment for matched variables, participants in the highest compared with the lowest quintile of adiponectin levels had a significantly decreased risk of MI (relative risk [RR], 0.39; 95% confidence interval [CI], 0.23-0.64; P for trend Ͻ.001). Additional adjustment for family history of MI, body mass index, alcohol consumption, physical activity, and history of diabetes and hypertension did not substantively affect this relationship (RR, 0.41; 95% CI, 0.24-0.70; P for trend Ͻ.001). Further adjustment for hemoglobin A 1c or C-reactive protein levels also had little impact, but additional adjustment for low-and high-density lipoprotein cholesterol levels modestly attenuated this association (RR, 0.56; 95% CI, 0.32-0.99; P for trend=.02). Conclusions High plasma adiponectin concentrations are associated with lower risk of MI in men. This relationship can be only partly explained by differences in blood lipids and is independent of inflammation and glycemic status.

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Adiponectin: Stability in Plasma over 36 Hours and Within-Person Variation over 1 Year

Cited by 139 publications

References 30 publications

Sex-specific determinants of serum adiponectin in older adults: the role of endogenous sex hormones

Sex-specific determinants of serum adiponectin in older adults: the role of endogenous sex hormones

Effects of Delayed Sample Processing on Determination of Total and High Molecular Weight (HMW) Adiponectin in Serum and Plasma: A Pilot Study

Plasma Adiponectin Levels and Risk of Myocardial Infarction in Men

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