Adiponectin Gene Polymorphism and its Association with Type 2 Diabetes Mellitus

Abstract

Mutations in different regions of adiponectin gene have been reported to be associated with obesity, atherosclerosis and type 2 diabetes mellitus. The present study was aimed to investigate the association among SNP 45 T [ G of adiponectin gene and type 2 diabetes in South Indian population. 75 clinically diagnosed case of type 2 diabetes were studied and compared with 75 apparently healthy controls. The genotype frequency of SNP45 T [ G in exon 2 of adiponectin gene was determined by PCR based restriction enzyme analysis using the restriction enzyme SmaI. (recognition site: CCC;GGG). Three kind of genotypes: wild type TT (470 bp), heterozygous type TG (470 bp, 336 bp, 134 bp) and homozygote mutant type GG (336 bp, 134 bp) were studied. A positive association has been found between SNP45 T [ G and type 2 diabetes in the study population (P = 0.010, OR = 3.797, 95% CI = 1.312-10.983). Therefore, SNP45T [ G in adiponectin gene may be one of the risk factors for type 2 diabetes.

Association of Single Nucleotide Polymorphisms of Adiponectin Gene with Type 2 Diabetes Mellitus, and Their Influence on Cardiovascular Risk Markers

“…In South Indian population Biswas et al [15] have shown a positive association between SNP ?45T/G and type 2 diabetes mellitus with the odds ratio of 3.797. A study in Romanian patients to assess possible link to prevalence of type 2 diabetes mellitus, demonstrated no genotype difference between type 2 diabetics and controls.…”

“…Government Medical College, Pune. The sample size was calculated from the previous study of SNP ?45T/G done in Indian population by Biswas D et al [15]. 150 patients of diabetes mellitus (diagnosed as per ADA guidelines) in the period of 2011-2013 were selected by simple random sampling method from out patients Department of Medicine, B. J.…”

Gene Polymorphism of Adiponectin SNP + 45 with Reference to Thyroid Status - A Comparative Study among Non-Obese, Overweight and Obese Type 2 Diabetics

“…Furthermore, the same study delineated the observation that a statistically significant association was observed between the anthropometric indices BMI and WC and T2DM. We primarily focused on gene polymorphism pertaining to SNP + 45 of adiponectin as linked to insulin resistance in the three groups of type 2 diabetics, namely non-obese, overweight and obese, based on a previous report from South India on SNP + 45 of Adiponectin [13], but we carried out the study with reference to thyroid status.…”

“…An Indian study depicted positive association of a particular polymorphism of the adiponectin gene, namely SNP + 45 T > G (rs2241766) with type 2 diabetes mellitus [13]. Although, altered thyroid profile is widely implicated in T2DM, it must be said in all fairness that limited documentation is available implicating adiponectin gene polymorphism in insulin resistance associated with altered thyroid status.…”

Tip 2 diyabetin moleküler genetik temeli; Son gelişmeler

“…Adipositlerde ifade edilen adiponektin geni bir başka aday gen olarak bir çok popülasyonda çalışıldı ve insülin hassasiyetin ayarlanmasında iş gören protein olan adiponektin varyantlarının (özellikle −11391G>A, −11377C>G, +45T>G, ve +276G>T) insülin direnci ve T2DM gelişi-miyle ilişkisi bir çok grup tarafından rapor edildi (53)(54)(55)(56)(57)(58)(59)(60). Diğer taraftan, ENPP1 (ectonucleotide pyrophosphatase/ phosphodiesterase 1) geni ve onun K121Q varyantı da insülin direnci ve T2DM ile kuvvetli ilişkilendirildi (61).…”