Adipogenic differentiation of human induced pluripotent stem cells: Comparison with that of human embryonic stem cells

Abstract

a b s t r a c tInduced pluripotent stem (iPS) cells were recently established from human fibroblasts. In the present study we investigated the adipogenic differentiation properties of four human iPS cell lines and compared them with those of two human embryonic stem (ES) cell lines. After 12 days of embryoid body formation and an additional 10 days of differentiation on Poly-L-ornithine and fibronectincoated dishes with adipogenic differentiation medium, human iPS cells exhibited lipid accumulation and transcription of adipogenesis-related molecules such as C/EBPa, PPARc2, leptin and aP2. These results demonstrate that human iPS cells have an adipogenic potential comparable to human ES cells.

Browning of White Adipose Cells by Intermediate Metabolites: An Adaptive Mechanism to Alleviate Redox Pressure

“…This lactate-induced Ucp1 gene expression was not due to osmotic perturbations, as mannitol did not upregulate Ucp1 (data not shown). Importantly, lactate-mediated increase in thermogenic gene expression also occurred in human-induced pluripotent stem-derived adipocytes (29) (Fig. 2E), demonstrating lactate effect on human cells.…”

Augmentation of Musculoskeletal Regeneration: Role for Pluripotent Stem Cells

“…In vitro adipogenesis from PSCs has so far relied on the generation of EBs from ESCs and iPSCs, and subsequent differentiation into adipocytes [117][118][119]. Retinoic acid (RA) is well documented as being an inducer of adipogenesis.…”

“…Retinoic acid (RA) is well documented as being an inducer of adipogenesis. Using RA as the differentiation stimulus, a comparison of the adipogenic potential of ESCs and iPSCs has been performed [119]. The authors reported no significant variation between the adipogenic potential of hESCs and hiPSCs overall, however, there was heterogeneity between different iPSC cell lines, despite having the same genetic origin.…”

Mesenchymal Stem Cells: Possibilities of New Treatment Options

“…Examples are auditory retinal cells (Jin et al 2009 ;Comyn et al 2010 ;Lamba et al 2010 ;Parameswaran et al 2010 ) , cardiomyocytes (Narazaki et al 2008 ;Pfannkuche et al 2009 ;Tanaka et al 2009 ;Zhang et al 2009b ;CarvajalVergara et al 2010 ;Kaichi et al 2010 ;Martinez-Fernandez et al 2010 ) , insulinsecreting islet-like clusters (Tateishi et al 2008 ) , motor neurons (Dimos et al 2008 ;Ebert et al 2009 ;Hu and Zhang 2009 ;Karumbayaram et al 2009 ) , dopaminergic neurons (Wernig et al 2008 ;Cai et al 2010 ;Swistowski et al 2010 ) , auditory spinal ganglion neurons (Nishimura et al 2009 ) , smooth muscle cells (Taura et al 2009b ;Xie et al 2009 ) , vascular endothelial cells (Taura et al 2009b ) , dendritic cells and macrophages (Senju et al 2009(Senju et al , 2010 , adipocytes (Tashiro et al 2009 ;Taura et al 2009a ) , osteoblasts (Tashiro et al 2009 ) , hematopoietic cells (Hanna et al 2007 ;Eminli et al 2009 ;Kaufman 2009 ;Lu et al 2009 ;Okabe et al 2009 ;Raya et al 2009 ;Kaneko et al 2010 ) , and endothelial progenitor cells (Xu et al 2009 ;Abaci et al 2010 ;…”