Adipocytes utilize sucrose as an energy source—Effect of different carbohydrates on adipocyte differentiation

Krishna, Mahesh S.; Revathy, V. M.; Jaleel, Abdul

doi:10.1002/jcp.29003

Cited by 10 publications

(9 citation statements)

References 37 publications

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“…Glucose is a main substrate for lipid synthesis in adipocytes, and its metabolic intermediate glucose-6-phosphate enhances the expression and transcriptional activity of ChREBP to promote adipogenesis [ 5 ]. In line with previous findings [ 6 ], HG significantly increased lipid accumulation in porcine adipocytes. Here, we focused on the regulatory effect of glucose on adipogenesis in porcine adipocytes, and identified 843 DEGs (628 upregulated and 215 downregulated) and 365 DAMs (233 upregulated and 132 downregulated) between LG and HG groups.…”

Section: Discussionsupporting

confidence: 93%

“…This differentiation process is strictly regulated by a series of adipogenic transcriptional factors [ 4 ]. These factors, such as peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer binding proteins (C/EBPs), and carbohydrate responsive element binding proteins (ChREBP), were considered to be central regulators of glucose-induced adipogenesis in adipocytes [ 5 , 6 ]. PPARγ is a ligand-activated transcription factor that participates in lipid metabolism, glucose homeostasis, immune responses, and inflammation during different metabolic states [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome and Metabolome Analyses Provide Insight into the Glucose-Induced Adipogenesis in Porcine Adipocytes

Jiang,

Zhang,

Miao

et al. 2024

CIMB

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Glucose is a major energy substrate for porcine adipocytes and also serves as a regulatory signal for adipogenesis and lipid metabolism. In this study, we combined transcriptome and metabolome analyses to reveal the underlying regulatory mechanisms of high glucose (HG) on adipogenesis by comparing differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) identified in porcine adipocytes. Results showed that HG (20 mmol/L) significantly increased fat accumulation in porcine adipocytes compared to low glucose (LG, 5 mmol/L). A total of 843 DEGs and 365 DAMs were identified. Functional enrichment analyses of DEGs found that multiple pathways were related to adipogenesis, lipid metabolism, and immune-inflammatory responses. PPARγ, C/EBPα, ChREBP, and FOS were identified as the key hub genes through module 3 analysis, and PPARγ acted as a central regulator by linking genes involved in lipid metabolism and immune-inflammatory responses. Gene-metabolite networks found that PPARγ-13-HODE was the most important interaction relationship. These results revealed that PPARγ could mediate the cross-talk between adipogenesis and the immune-inflammatory response during adipocyte maturation. This work provides a comprehensive view of the regulatory mechanisms of glucose on adipogenesis in porcine adipocytes.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Transcriptome and Metabolome Analyses Provide Insight into the Glucose-Induced Adipogenesis in Porcine Adipocytes

Jiang,

Zhang,

Miao

et al. 2024

CIMB

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“…In fact, most characterizing components, such as furanocoumarins were present only in FCa, which showed anti-obesity potentiality, while their absence was associated with reduced effectiveness: an almost opposite effect was observed. Furthermore, FCs NMR spectrum also revealed a higher presence of sucrose and glucose, two well-known energy sources involved in increasing the expression of pro-adipogenic regulators and fat storage [ 53 , 57 ], suggesting a highly relevant contributing role in exerting FCs effects on 3T3-L1 cells.…”

Section: Discussionmentioning

confidence: 99%

Different Seasonal Collections of Ficus carica L. Leaves Diversely Modulate Lipid Metabolism and Adipogenesis in 3T3-L1 Adipocytes

et al. 2022

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Due to the high prevalence of obesity and type 2 diabetes, adipogenesis dysfunction and metabolic disorders are common features in the elderly population. Thus, the identification of novel compounds with anti-adipogenic and lipolytic effects is highly desirable to reduce diabetes complications. Plants represent an important source of bioactive compounds. To date, the antidiabetic potential of several traditional plants has been reported, among which Ficus carica L. is one of the most promising. Considering that plant metabolome changes in response to a number of factors including seasonality, the aim of this study was to evaluate whether Ficus carica leaves extracts collected in autumn (FCa) and spring (FCs) differently modulate lipid metabolism and adipogenesis in 3T3-L1 adipocytes. The 1H-NMR profile of the extracts showed that FCs have a higher content of caffeic acid derivatives, glucose, and sucrose than FCa. In contrast, FCa showed a higher concentration of malic acid and furanocoumarins, identified as psoralen and bergapten. In vitro testing showed that only FCa treatments were able to significantly decrease the lipid content (Ctrl vs. FCa 25 μg/mL, 50 μg/mL and 80 μg/mL; p < 0.05, p < 0.01 and p < 0.001, respectively). Furthermore, FCa treatments were able to downregulate the transcriptional pathway of adipogenesis and insulin sensitivity in 3T3-L1 adipocytes. In more detail, FCa 80 μg/mL significantly decreased the gene expression of PPARγ (p < 0.05), C/EBPα (p < 0.05), Leptin (p < 0.0001), adiponectin (p < 0.05) and GLUT4 (p < 0.01). In conclusion, this study further supports an in-depth investigation of F. carica leaves extracts as a promising source of active compounds useful for targeting obesity and diabetes.

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“…In both physiological and pathological contexts, adipogenesis is known to be highly sensitive to the nutritional composition. Especially, changes in the carbohydrates resources are expected to modulate this differentiation process [ 40 , 42 , 51 , 52 , 53 ]. As obesity and co-morbidities are increasing worldwide, (re)considering the nutritional qualitative and quantitative parameters as a strategy to improve the management of those diseases seems to be decisive.…”

Section: Discussionmentioning

confidence: 99%

Influence of Nutritional Intake of Carbohydrates on Mitochondrial Structure, Dynamics, and Functions during Adipogenesis

et al. 2020

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Obesity is an alarming yet increasing phenomenon worldwide, and more effective obesity management strategies have become essential. In addition to the numerous anti-adipogenic treatments promising a restauration of a healthy white adipose tissue (WAT) function, numerous studies reported on the critical role of nutritional parameters in obesity development. In a metabolic disorder context, a better control of nutrient intake is a key step in slowing down adipogenesis and therefore obesity. Of interest, the effect on WAT remodeling deserves deeper investigations. Among the different actors of WAT plasticity, the mitochondrial network plays a central role due to its dynamics and essential cellular functions. Hence, the present in vitro study, conducted on the 3T3-L1 cell line, aimed at evaluating the incidence of modulating the carbohydrates intake on adipogenesis through an integrated assessment of mitochondrial structure, dynamics, and functions-correlated changes. For this purpose, our experimental strategy was to compare the occurrence of adipogenesis in 3T3-L1 cells cultured either in a high-glucose (HG) medium (25 mM) or in a low-glucose (LG) medium (5 mM) supplemented with equivalent galactose (GAL) levels (20 mM). The present LG-GAL condition was associated, in differentiating adipocytes, to a reduced lipid droplet network, lower expressions of early and late adipogenic genes and proteins, an increased mitochondrial network with higher biogenesis marker expression, an equilibrium in the mitochondrial fusion/fission pattern, and a decreased expression of mitochondrial metabolic overload protein markers. Therefore, those main findings show a clear effect of modulating glucose accessibility on 3T3-L1 adipogenesis through a combined effect of adipogenesis modulation and overall improvement of the mitochondrial health status. This nutritional approach offers promising opportunities in the control and prevention of obesity.

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Adipocytes utilize sucrose as an energy source—Effect of different carbohydrates on adipocyte differentiation

Cited by 10 publications

References 37 publications

Transcriptome and Metabolome Analyses Provide Insight into the Glucose-Induced Adipogenesis in Porcine Adipocytes

Transcriptome and Metabolome Analyses Provide Insight into the Glucose-Induced Adipogenesis in Porcine Adipocytes

Different Seasonal Collections of Ficus carica L. Leaves Diversely Modulate Lipid Metabolism and Adipogenesis in 3T3-L1 Adipocytes

Influence of Nutritional Intake of Carbohydrates on Mitochondrial Structure, Dynamics, and Functions during Adipogenesis

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