AdhO36 Liposomes from Salmonella Typhi in Combination With β-Glucan Immuno-adjuvant From Candida albicans Cell Wall as Oral Vaccine Against Typhoid Fever in Mice Model

Rachmawati, Hidajah; Winarsih, Sri; Prawiro, Sumarno Reto; Barlıanto, Wisnu; Santoso, Sanarto; Djunaedi, Djoni; Endharti, Agustina Tri; Sardjono, Teguh Wahyu; Khotimah, Husnul; Prihanti, Gita Sekar; Nugraheni, Raditya Weka; Sumadi, Firasti Agung Nugrahening; Yusuf, Helmy

doi:10.3889/oamjms.2020.4422

Cited by 5 publications

(3 citation statements)

References 27 publications

(28 reference statements)

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“…We chose the ISCOM molecule. Our results show that ISCOM containing β-Glucan from C. albicans and AdhO36 S. Typhi has a potential effect on cellular and humoral immune response (Rachmawati et al, 2020).…”

Section: Introductionmentioning

confidence: 71%

Generating Responses Immune in Cellular and Humoral Treatment With Epitope Spike, Epitope Envelope Protein, and Epitope Membrane Protein Sars-Cov-2, Honey, Saussurea Lappa, and Nigella Sativa

Prawiro¹,

Kusuma²,

Amiruddin³

et al. 2021

AJID

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Background:Covid-19 has become pandemic in the World, including Indonesia. Our last study showed that HSF could serve as an immunomodulator. Using the exact search, we found that the most immuno-dominant SARS-COV2 epitope, namely A spike protein epitope, B envelope protein epitope, and C membrane protein epitope, we concise to be HF Materials and Methods:We used to post only control design study and mice as an animal model. The research divided mice into four groups, and the first group as control received PBS as a placebo. The second, three, and last four groups gave HF, HSN, and HFHSN (combine HF and HSN). All of the regiment enters the mouth with a special sonde to reach the gastrointestinal organ. We gave HF every week three times and HSN once a day. After administration regiments for a long three weeks, we sacrificed the mice. We evaluated cellular immune responses that are Th-2, Th-17, and NK cells. We check for humoral immune response, TGF-β,IL-17A, IL-4, IgG,IL-4, β-defensin, and s-IgA. Results:Highest profile cellular immunity HF, HSN, and HFHSN were NK cell, Th-2 and Th-17, and the last NK cell, respectively. After that which in humoral immunity, the domination response IgG and IL-4 were HF. But HSN and HFHSN dominated for s-IgA and β-defensin production. By using the study Bio-Informatica, we found HF. Conclusion: If the results of this study are continued to the clinical trial level, it is necessary to recommend additional markers such as CTL (s-IgA and β-defensin in lung tissue)and CPE assay.

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Section: Introductionmentioning

confidence: 71%

Generating Responses Immune in Cellular and Humoral Treatment With Epitope Spike, Epitope Envelope Protein, and Epitope Membrane Protein Sars-Cov-2, Honey, Saussurea Lappa, and Nigella Sativa

Prawiro¹,

Kusuma²,

Amiruddin³

et al. 2021

AJID

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“…Several publications report alum mechanisms such as creating a gel depot that prolonged exposure of the immune system to antigen, forming particulate structures that promote antigen uptake by APCs (Antigen Presenting Cells) via phagocytosis, and inducing inflammation and secretion of chemokines. However, its inability to induce Th1 cell-mediated immune responses also becomes a limitation for this adjuvant [26], Since the previous study indicated that the typhoid sub-unit vaccine-induced cellular immunity through TCD4+ [27].…”

Section: Epitope Vaccine Deliverymentioning

confidence: 99%

In-Silico Approach in the Development of Salmonella Epitope Vaccine

Rachmawati¹,

Nugraheni²,

Sumadi³

2021

Salmonella Spp. - A Global Challenge

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In the case of infection control, one of our primary concerns is typhoid fever. According to WHO, typhoid prevalence in Indonesia is highly endemic. There is also the problem with the low efficacy of the available vaccine to prevent the disease. Therefore, there is an urgent need to develop a highly effective typhoid vaccine. One of the phases in vaccine development is an exploratory phase, a research-intensive phase of the vaccine development process designed to identify natural or synthetic antigens that might help prevent or treat a disease through computer in silico prediction targets. The vaccines developed through epitope peptide are designed to be safer, more efficacious, and less expensive than traditional vaccines. A thorough understanding of the disease agent, particularly critical epitopes to induce the appropriate immunological reaction, is required to achieve these aims. Mapping epitope sequences or antigenic peptides from pathogenic proteins recognized by B cells and T cells is crucial for vaccine development. Once the epitopes were identified, the polypeptide production could be produced through protein recombinant technology. The polypeptide vaccine, in the end, could be delivered using a liposomal delivery system.

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“…The physical stability of DDAB is low because it is aggregated quickly in salty liquids, even at low concentrations (Nugraheni et al, 2020). Our previous studies showed the effectiveness of the combination between liposomes containing β-Glucan from C. albicans and AdhO36 S. typhi, which potentially affects cellular and humoral immune response (Rachmawati et al, 2020).…”

Section: Particle Size Analysis and Zeta Potentialmentioning

confidence: 99%

Formulations of liposomal vaccine containing AdhO36 antigen and β-glucan as immunoadjuvant

et al. 2021

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AdhO36 Liposomes from Salmonella Typhi in Combination With β-Glucan Immuno-adjuvant From Candida albicans Cell Wall as Oral Vaccine Against Typhoid Fever in Mice Model

Cited by 5 publications

References 27 publications

Generating Responses Immune in Cellular and Humoral Treatment With Epitope Spike, Epitope Envelope Protein, and Epitope Membrane Protein Sars-Cov-2, Honey, Saussurea Lappa, and Nigella Sativa

Generating Responses Immune in Cellular and Humoral Treatment With Epitope Spike, Epitope Envelope Protein, and Epitope Membrane Protein Sars-Cov-2, Honey, Saussurea Lappa, and Nigella Sativa

In-Silico Approach in the Development of Salmonella Epitope Vaccine

Formulations of liposomal vaccine containing AdhO36 antigen and β-glucan as immunoadjuvant

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