Adhesive resistance of a copaiba oil-based dentin biomodifier

Abstract

This study analyzed the effect of prior application of copaiba oil (CO) emulsions as a dentin cleaning substance on microleakage and microtensile adhesive strength. Twenty-five premolars and sixty-four molars were used for microleakage and microtensile assays. For the microleakage assays, specimens with standard class V cavities were divided (n = 5), according to the tested CO emulsions: CO10%X, CO10%Y, and CO10%Z, as well as chlorhexidine 2% (CHX) and distilled water (DW), as positive and negative controls, respectively. Restorations were performed using the Adper Single Bond® and/or Clearfil SE Bond® systems. Cervical, occlusal, distal and mesial sections were assessed for tracer penetration degree at the composite/tooth interface. For the microtensile assay, healthy molars were divided into sixteen groups, in which artificial caries were induced in half of the groups. Dentin surfaces were treated with CO10%X and CO10%Y, CHX and DW. Microtensile bond strength was measured by fixing each sample to the plate of a universal testing machine operated at a speed of 0.5 mm/minute until failure. Dentin treated with CO10%X showed a lower infiltration rate than dentin treated with the other CO emulsions, CHX2% and DW. According to the microtensile assay, both healthy and affected dentin treated with CO10%X and Adper Single Bond® adhesive system presented higher adhesive strength. CO emulsion, used as a dentin biomodifier, interfered positively in microleakage and improved adhesive strength after acid etching in the Adper Single Bond® adhesive system, or before applying the Clearfil SE Bond® self-etching system.

Atividade Do Óleo De Copaíba Sobre Radicais Livres Formados Durante a Resposta Inflamatória

“…Emulsões à base de óleo de copaíba usadas antes da aplicação do adesivo provocaram a inibição de MMPs, contribuindo de forma significativa na estabilidade da camada híbrida, impedindo a hidrólise do colágeno e melhorando a durabilidade da ligação resina-dentina (Bandeira et al, 2016;Bandeira et al, 2020).…”

“…Pode ser formulado em emulsão (De Bari et al, 2016;Bandeira et al, 2020), gel de limpeza de cavidade (Simões et al, 2016), cimento odontológico (Vasconcelos et al, 2008) e cimento endodôntico experimental (Garrido et al, 2015).…”

“…O gênero Copaifera L. tem sido estudado devido às suas várias atividades biológicas, onde o efeito anti-inflamatório, decorrente do sinergismo entre os constituintes do óleo-resina, é um dos mais investigados (Izuma et al, 2012). Possui também atividades antimicrobiana, germicida, analgésica, antineoplásica, com potencial aplicabilidade na Odontologia (Bandeira et al, 1999;Vasconcelos et al, 2008;Veiga Jr. et al, 2007; Yamagushi e Garcia, 2012; De Bari et al, 2016;Bandeira et al, 2016) e, mais recentemente, antiproteolítica, agindo sobre as metaloproteinases MMP2 e MMP9, presentes no tecido dentinário (Bandeira et al, 2016;Bandeira et al, 2020).…”

“…Considerando as supracitadas propriedades da copaíba, parece razoável hipotetizar que sua emulsão, além de favorecer a adesão do material restaurador à estrutura dentária (Bandeira et al, 2020) devido a sua ação biomodificadora do tecido dentinário, possua atividade anti-inflamatória e seja capaz de atenuar a possível inflamação pulpar, objetivando-se futuramente sua ação terapêutica em cavidades profundas em substituição ao material disponível no mercado brasileiro (clorexidina), que não apresenta tal propriedade.…”

Effect of a Copaiba Oil-Based Dental Biomodifier on the Inhibition of Metalloproteinase in Adhesive Restoration

“…Copaiba, whose oil has been the subject of research, shows a promising solution, exposing significant results in cavity cleaning, bactericidal, and bacteriostatic potential similar to the 2% chlorhexidine digluconate solution [ 12 ]. Studies have shown its efficiency in maintaining adhesive strength [ 13 ], presenting a homogeneous, regular, continuous hybrid layer, and the presence of tags independent of the dentin substrate [ 13 , 14 ].…”