Adhesion to stainless steel surfaces and detection of viable but non cultivable cells of Vibrio parahaemolyticus and Vibrio cholerae isolated from shrimps in seafood processing environments: Stayin’ alive?

Mougin, Julia; Copin, S.; Bojolly, Daline; Raguenet, Virginie; Robert-Pillot, Annick; Quilici, Marie‐Laure; Midelet, Graziella; Grard, Thierry; Bonnin-Jusserand, Maryse

doi:10.1016/j.foodcont.2019.03.024

Cited by 29 publications

(11 citation statements)

References 39 publications

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“…Given the wide consumption of fishery products in Algeria and the low number of literature data on Vibrio spp. as pathogenic agents in Algeria and beyond, it is clearly important to understand their distribution in marine environments as well as to develop methodologies for detecting viable but non-culturable forms of these organisms [ 59 ].…”

Section: Resultsmentioning

confidence: 99%

Occurrence of Vibrio spp. along the Algerian Mediterranean coast in wild and farmed Sparus aurata and Dicentrarchus labrax

et al. 2020

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Background and Aim: Vibrio bacteria are autochthonous inhabitants of aquatic and marine environments. Certain strains are responsible for important seafood-borne outbreaks in developed nations. The aim of this study was to investigate the occurrence of Vibrio spp. along the Algerian Mediterranean coast in several samples of two prominent wild and farmed marine fishes, Sparus aurata and Dicentrarchus labrax. Materials and Methods: A total of 350 S. aurata (n=280 farmed and n=70 wild) and 340 D. labrax (n=250 farmed and n=90 wild) were sampled among three different locations along the Algerian Mediterranean coastal area. The samples were processed for Vibrio research according to the ISO methods. Isolated colonies were identified utilizing biochemical tests and consecutively confirmed with matrix-assisted laser desorption ionization-time-of-flight mass spectrometry, combined with polymerase chain reaction (PCR) analysis when appropriate, or confirmed with PCR analysis alone. Results: A total of 42 Vibrio spp. were detected only among the farmed fishes. Taking into account, all 690 fishes sampled, the incidence of Vibrio spp. was 6.08% (with peaks up to 7.92%) among the total number of farmed fishes. Overall, 25 strains were isolated from S. aurata and 17 strains were isolated from D. labrax. The isolated strains belonged to four different species and were represented as follows: Vibrio alginolyticus (n=20), Vibrio cholerae (n=15), Vibrio fluvialis (n=5), and Vibrio hollisae (n=2). The incidence of Vibrio was higher in places characterized by greater levels of anthropogenic contamination of seawater. Conclusion: Considering the growing production and consequent rising consumption of farmed fish in Algeria, the reported incidence of Vibrio and the presence of potentially pathogenic strains of Vibrio such as V. cholerae cause particular concern for food safety matters. Even if innovative and natural techniques are desired in aquaculture, proper hygiene and manufacturing practices are essential for the correct management of Vibrio infection risk in farmed fishes at both industrial and domestic levels.

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Section: Resultsmentioning

confidence: 99%

Occurrence of Vibrio spp. along the Algerian Mediterranean coast in wild and farmed Sparus aurata and Dicentrarchus labrax

et al. 2020

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“…Such significant difference was collectively contributed by the following factors including the bacterial species, the type of attachment surfaces, and the environmental conditions (pH, temperature, nutritional conditions) (Whitehead and Verran, 2015). Among these factors, the bacterial species appears to act a pivotal role in biofilm formation owing to the heterogeneity of Vibrio strains (Song et al, 2016;Odeyemi and Ahmad, 2017;Mougin et al, 2019). Once a mature biofilm community is established, the resistance to the harsh environments will be strengthened due to the functions of EPS (Nilsson et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Vibrio parahaemolyticus is recognized as a leading cause of seafood-derived food poisoning worldwide (Raszl et al, 2016), and it is ubiquitous in coastal waters or estuarine environments (Urmersbach et al, 2015;Mizan et al, 2016). V. parahaemolyticus has the high capacity to adhere to food-contact surfaces (aquaculture equipment, aquatic products, and food processing facilities) and forms biofilm (Han et al, 2016;da Rosa et al, 2018;Mougin et al, 2019). Biofilms are complex communities of microorganisms, which provides the encased microbial cells higher ability to tolerate environmental stresses such as antibiotics and disinfectants compared to planktonic cells (Costerton et al, 1999;Costa et al, 2013;Elexson et al, 2014;DeFrancesco et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Insights Into the Role of Extracellular DNA and Extracellular Proteins in Biofilm Formation of Vibrio parahaemolyticus

Wang

Qian

et al. 2020

Front. Microbiol.

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The extracellular polymeric substances (EPS) construct the three-dimensional (3-D) structure of biofilms, but their respective roles are still not clear. Therefore, this study aimed to illuminate the role of key chemical components [extracellular DNA (eDNA), extracellular proteins, and carbohydrates] of EPS in biofilm formation of Vibrio parahaemolyticus. The correlations between each key chemical component and biofilm formation were first determined, showing that the biofilm formation of V. parahaemolyticus was strongly positively correlated with both eDNA and protein content (P < 0.01), but not with carbohydrates. Subsequently, individual DNase I or protease K treatment markedly reduced the initial adhesion and structural stability of the formed biofilms by hydrolyzing the eDNA or extracellular proteins, but did not induce significant dispersion of mature biofilms. However, the combination of DNase I and protease K treatment induced the obvious dispersion of the mature biofilms through the concurrent destruction of eDNA and extracellular proteins. The analysis at a structural level showed that the collapse of biofilms was mainly attributed to the great damage of the loop configuration of eDNA and the secondary structure of proteins caused by the enzyme treatment. Therefore, this study provides a deep understanding of the role of key chemical components of EPS in biofilm development of V. parahaemolyticus, which may give a new strategy to develop environmentally friendly methods to eradicate the biofilms in food industry.

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“…In this context, reliable detection will become increasingly important. It is also probable that in the near future, further studies will be needed to optimize PMA treatment combined with qPCR in addition to conventional microbiological methods to reduce the risk of human pathogenic Vibrio in seafood companies (Mougin et al 2019), as well as to reduce vibriosis in aquaculture. In conclusion, regarding the results of our study, PMA did not show any lethal effect against Vibrio sp.…”

Section: Resultsmentioning

confidence: 99%

Ethidium and propidium monoazide: comparison of potential toxicity onVibriosp. viability

Copin

Mougin

Raguenet

et al. 2020

Lett Appl Microbiol

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Significance and Impact of the Study: Ethidium monoazide (EMA) and propidium monoazide (PMA) treatment have commonly been used combined with qPCR to discriminate viable and dead cells. However, these DNA-binding dyes can damage membranes in viable bacteria, generating false-negative results. Most available studies examined either EMA-qPCR or PMA-qPCR, based on the literature to select the dye of interest. However, none of them tested the cytotoxicity of EMA vs PMA on various Vibrio sp. strains. In this study, we showed that PMA was the optimal dye for Vibrio as a tool for pretreatment when using qPCR methods to determine Vibrio viability in environmental or seafood samples.

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Adhesion to stainless steel surfaces and detection of viable but non cultivable cells of Vibrio parahaemolyticus and Vibrio cholerae isolated from shrimps in seafood processing environments: Stayin’ alive?

Cited by 29 publications

References 39 publications

Occurrence of Vibrio spp. along the Algerian Mediterranean coast in wild and farmed Sparus aurata and Dicentrarchus labrax

Occurrence of Vibrio spp. along the Algerian Mediterranean coast in wild and farmed Sparus aurata and Dicentrarchus labrax

Insights Into the Role of Extracellular DNA and Extracellular Proteins in Biofilm Formation of Vibrio parahaemolyticus

Ethidium and propidium monoazide: comparison of potential toxicity onVibriosp. viability

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