Adhesion of Soft Membranes Controlled by Tension and Interfacial Polymers

Sengupta, Kheya; Limozin, Laurent

doi:10.1103/physrevlett.104.088101

Cited by 49 publications

(84 citation statements)

References 27 publications

(11 reference statements)

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“…Knowing the refractive indices and thicknesses of the different layers and using Fresnel's equations the detected intensity I(h) is calculated. The theory of partial coherent light states that these data follow the intensity-height relation 52 IðhÞ…”

Section: Ricmmentioning

confidence: 99%

“…This behaviour is different from previous studies on GUVs adhering strongly to homogeneous substrates -as noticeable by the suppression of fluctuations within the adhesion zone and tension increase. 52,84 On homogeneous substrates fluctuations bring a new membrane area into contact with the substrate and cause growth of the adhesion zone. In the present case the nonadhesive square areas on the substrate are so large that fluctuations cannot reach to the other side to establish new contacts.…”

Section: Soft Matter Papermentioning

confidence: 99%

“…For example, in the limit of strong adhesion, the classical contact angle equation by YoungDupré 48 can be used to determine the lateral membrane tension and the free energy of adhesion per unit area W, where W p ÀV(h). 19,30,[49][50][51][52] Another strategy in the case of ultra weak gravity dominated confinement is to use the overall shape deformation of the GUV. [52][53][54] Recently, we used the local equilibrium shape of the membrane to extract the detailed form of the interaction potential for the case of a membrane adhered to micropatterned substrates.…”

Section: Introductionmentioning

confidence: 99%

“…19,30,[49][50][51][52] Another strategy in the case of ultra weak gravity dominated confinement is to use the overall shape deformation of the GUV. [52][53][54] Recently, we used the local equilibrium shape of the membrane to extract the detailed form of the interaction potential for the case of a membrane adhered to micropatterned substrates. 55 In the present study, a general, case-independent strategy is presented where g is determined from the differences of fluctuations measured in the vicinity of the substrate and far away from it.…”

Section: Introductionmentioning

confidence: 99%

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Nanometric thermal fluctuations of weakly confined biomembranes measured with microsecond time-resolution

et al. 2016

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We probe the bending fluctuations of bio-membranes using highly deflated giant unilamellar vesicles (GUVs) bound to a substrate by a weak potential arising from generic interactions. The substrate is either homogeneous, with GUVs bound only by the weak potential, or is chemically functionalized with a micro-pattern of very strong specific binders. In both cases, the weakly adhered membrane is seen to be confined at a well-defined distance above the surface while it continues to fluctuate strongly. We quantify the fluctuations of the weakly confined membrane at the substrate proximal surface as well as of the free membrane at the distal surface of the same GUV. This strategy enables us to probe in detail the damping of fluctuations in the presence of the substrate, and to independently measure the membrane tension and the strength of the generic interaction potential. Measurements were done using two complementary techniques -dynamic optical displacement spectroscopy (DODS, resolution: 20 nm, 10 ms), and dual wavelength reflection interference contrast microscopy (DW-RICM, resolution: 4 nm, 50 ms). After accounting for the spatio-temporal resolution of the techniques, an excellent agreement between the two measurements was obtained. For both weakly confined systems we explore in detail the link between fluctuations on the one hand and membrane tension and the interaction potential on the other hand.

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Section: Ricmmentioning

confidence: 99%

Section: Soft Matter Papermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Nanometric thermal fluctuations of weakly confined biomembranes measured with microsecond time-resolution

et al. 2016

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“…Its adsorption behavior is significant to study the properties of cell membrane [30,31]. TIRIE biosensor performed in null and off-null modes is applied to visualize vesicles (made of 1, 2-Dioleoyl-sn-glycero-3-phosphocholine) non-specific adsorption and desorption on poly-L-lysine modified gold surface shown in Fig.…”

Section: Vesicles Adsorption Process Visualizationmentioning

confidence: 99%

Development of biosensor based on imaging ellipsometry and biomedical applications

et al. 2011

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So far, combined with a microfluidic reactor array system, an engineering system of biosensor based on imaging ellipsometry is installed for biomedical applications, such as antibody screen, hepatitis B markers detection, cancer markers spectrum and virus recognition, etc. Furthermore, the biosensor in total internal reflection (TIR) mode has be improved by a spectroscopic light, optimization settings of polarization and low noise CCD which brings an obvious improvement of 10 time increase in the sensitivity and SNR, and 50 times lower concentration in the detection limit with a throughput of 48 independent channels and the time resolution of 0.04 S.

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