Adhesion of slime producing Staphylococcus epidermidis strains to PVC and diamond-like carbon/silver/fluorinated coatings

Katsikogianni, Maria G.; Spiliopoulou, Iris; Dowling, Denis P.; Missirlis, Yannis F.

doi:10.1007/s10856-006-9678-8

Cited by 74 publications

(69 citation statements)

References 29 publications

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“…Glass, Plexiglas (polymethylmethacrylate), and polyvinyl chloride (PVC) plates (30 by 30 cm), with different degrees of surface hydrophilicity (glass Ͼ Plexiglas Ͼ PVC) according to water contact angle measurements obtained previously (26,50), were used as test surfaces. So that data could be compared, a construct similar to that used in a previous experiment (15) was employed to deploy the surfaces in seawater at a depth of 1 m below the water surface near Wheat Island off the Qingdao coast (36.31°N, 120.25°E) on 23 May 2005.…”

Section: Methodsmentioning

confidence: 99%

Cross-Ocean Distribution of Rhodobacterales Bacteria as Primary Surface Colonizers in Temperate Coastal Marine Waters

Dang

Chen

et al. 2008

Appl Environ Microbiol

347

279

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Bacterial surface colonization is a universal adaptation strategy in aquatic environments. However, neither the identities of early colonizers nor the temporal changes in surface assemblages are well understood. To determine the identities of the most common bacterial primary colonizers and to assess the succession process, if any, of the bacterial assemblages during early stages of surface colonization in coastal water of the West Pacific Ocean, nonnutritive inert materials (glass, Plexiglas, and polyvinyl chloride) were employed as test surfaces and incubated in seawater off the Qingdao coast in the spring of 2005 for 24 and 72 h. Phylogenetic analysis of the 16S rRNA gene sequences amplified from the recovered surface-colonizing microbiota indicated that diverse bacteria colonized the submerged surfaces. Multivariate statistical cluster analyses indicated that the succession of early surface-colonizing bacterial assemblages followed sequential steps on all types of test surfaces. The Rhodobacterales, especially the marine Roseobacter clade members, formed the most common and dominant primary surface-colonizing bacterial group. Our current data, along with previous studies of the Atlantic coast, indicate that the Rhodobacterales bacteria are the dominant and ubiquitous primary surface colonizers in temperate coastal waters of the world and that microbial surface colonization follows a succession sequence. A conceptual model is proposed based on these findings, which may have important implications for understanding the structure, dynamics, and function of marine biofilms and for developing strategies to harness or control surface-associated microbial communities.

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Section: Methodsmentioning

confidence: 99%

Cross-Ocean Distribution of Rhodobacterales Bacteria as Primary Surface Colonizers in Temperate Coastal Marine Waters

Dang

Chen

et al. 2008

Appl Environ Microbiol

347

279

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“…Although the competing mechanisms remain unclear, a large body of data suggests that both physical and chemical modifications of a material surface can be engineered to limit bacterial colonization. [5][6][7][8][9][10]12,13,20,22 For example, as shown in Figure 1, coating surgical-grade titanium alloy with a synthetic PEM of PAA and PAH reduced colony density of waterborne S. epidermidis bacteria by orders of magnitude after immersion in 10 7 bacteria/mL. Reduced colonization over both 2 and 4 h incubation timescales is relevant to medical procedure durations involving, for example, cardiac assist and orthopedic implant devices.…”

Section: Bacterial Colonization Can Be Reduced By Materialsmentioning

confidence: 99%

Substrata Mechanical Stiffness Can Regulate Adhesion of Viable Bacteria

et al. 2008

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The competing mechanisms that regulate adhesion of bacteria to surfaces and subsequent biofilm formation remain unclear, though nearly all studies have focused on the role of physical and chemical properties of the material surface. Given the large monetary and health costs of medical-device colonization and hospital-acquired infections due to bacteria, there is considerable interest in better understanding of material properties that can limit bacterial adhesion and viability. Here we employ weak polyelectrolyte multilayer (PEM) thin films comprised of poly(allylamine) hydrochloride (PAH) and poly(acrylic acid) (PAA), assembled over a range of conditions, to explore the physicochemical and mechanical characteristics of material surfaces controlling adhesion of Staphylococcus epidermidis bacteria and subsequent colony growth. Although it is increasingly appreciated that eukaryotic cells possess subcellular structures and biomolecular pathways to sense and respond to local chemomechanical environments, much less is known about mechanoselective adhesion of prokaryotes such as these bacteria. We find that adhesion of viable S. epidermidis correlates positively with the stiffness of these polymeric substrata, independently of the roughness, interaction energy, and charge density of these materials. Quantitatively similar trends observed for wild-type and actin analogue mutant Escherichia coli suggest that these results are not confined to only specific bacterial strains, shapes, or cell envelope types. These results indicate the plausibility of mechanoselective adhesion mechanisms in prokaryotes and suggest that mechanical stiffness of substrata materials represents an additional parameter that can regulate adhesion of and subsequent colonization by viable bacteria.

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“…The presence of oxygen has been reported to be advantageous for biological activity. 54 Although some reports have found that fluorine can enhance bacterial adhesion on the surface of biomaterials, 55,56 others have reported that fluorine shows antibacterial ability. 57,58 Our data indicate that the roughness of both TiO 2 nanotube surfaces was higher than that of the two control samples.…”

mentioning

confidence: 99%

Dual effects and mechanism of TiO2 nanotube arrays in reducing bacterial colonization and enhancing C3H10T1/2 cell adhesion

Peng¹,

Ni²,

Zheng³

et al. 2013

IJN

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Adhesion of slime producing Staphylococcus epidermidis strains to PVC and diamond-like carbon/silver/fluorinated coatings

Cited by 74 publications

References 29 publications

Cross-Ocean Distribution of Rhodobacterales Bacteria as Primary Surface Colonizers in Temperate Coastal Marine Waters

Cross-Ocean Distribution of Rhodobacterales Bacteria as Primary Surface Colonizers in Temperate Coastal Marine Waters

Substrata Mechanical Stiffness Can Regulate Adhesion of Viable Bacteria

Dual effects and mechanism of TiO2 nanotube arrays in reducing bacterial colonization and enhancing C3H10T1/2 cell adhesion

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