Adhesion of osteoblast-like cells on nanostructured hydroxyapatite

Okada, Shinnosuke; Ito, Hiroyuki; Nagai, Atsushi; Komotori, Jun; Imai, Hiroaki

doi:10.1016/j.actbio.2009.07.037

Cited by 120 publications

(90 citation statements)

References 29 publications

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“…The compactness and crystallinity of the FM-HA coating were lower, therefore a cell could only come into contact with the top of a few flake-like grains of hydroxyapatite, leaving most of the cell wall hanging in air. Okada et al pointed out that the formation of focal adhesion requires a flat domain size larger than 100 nm in width [19]. Massive cell apoptosis found on the FM-HA coating may be attributed to the coating barely meeting this requirement.…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton’s jelly in vitro

Guan

Shan

Shi

et al. 2014

Sci. China Life Sci.

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Seeding cells and scaffolds play pivotal roles in bone tissue engineering and regenerative medicine. Wharton's jelly-derived mesenchymal stem cells (WJCs) from human umbilical cord represent attractive and promising seeding cells in tissue regeneration and engineering for treatment applications. This study was carried out to explore the biocompatibility of scaffolds to seeding cells in vitro. Rod-like nano-hydroxyapatite (RN-HA) and flake-like micro-hydroxyapatite (FM-HA) coatings were prepared on Mg-Zn-Ca alloy substrates using micro-arc oxidation and electrochemical deposition. WJCs were utilized to investigate the cellular biocompatibility of Mg-Zn-Ca alloys after different surface modifications by observing the cell adhesion, morphology, proliferation, and osteoblastic differentiation. The in vitro results indicated that the RN-HA coating group was more suitable for cell proliferation and cell osteoblastic differentiation than the FM-HA group, demonstrating better biocompatibility. Our results suggested that the RN-HA coating on Mg-Zn-Ca alloy substrates might be of great potential in bone tissue engineering.nano-hydroxyapatite, Mg-Zn-Ca alloy, mesenchymal stem cells, Wharton's jelly, biocompatibility Citation:Guan FX, Ma SS, Shi XY, Ma X, Chi LK, Liang S, Cui YB, Wang ZB, Yao N, Guan SK, Yang B. Biocompatibility of nano-hydroxyapatite/ Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton's jelly in vitro.

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Section: Discussionmentioning

confidence: 99%

Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton’s jelly in vitro

Guan

Shan

Shi

et al. 2014

Sci. China Life Sci.

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“…14) In a previous study, we reported the response of osteoblastic cells to nanostructured HA. 15) The cellular activities decreased remarkably on the nanostructured HA with grains smaller than ca. 100 nm in size when the domain gaps were larger than several hundred nanometers.…”

Section: )mentioning

confidence: 99%

“…The pellets of nanosized fibrous and flaky HA crystals were produced through hydrolysis of a DCP pellet in alkaline solutions. 15),21) Since the HA nanocrystals had a calcium-deficient composition, a BCP phase was formed by the calcination of the nanostructured pellets, as shown in Fig. 1.…”

Section: Formation Of Bcp Pelletsmentioning

confidence: 99%

“…In our previous work, 15) the adhesion and the ALP activity of osteoblastic cells were reported to be remarkably suppressed on nanostructured HA surfaces. Especially, the presence of HA primary crystals under 100 nm in size led to apoptosis of the cells.…”

Section: Cellular Responsementioning

confidence: 99%

“…15) Commercial calcium phosphate dihydrate powder (Junsei Chemical) was treated in water at 80°C for 3 h to prepare calcium phosphate anhydride (DCP) as a precursor of the nanostructured HA. A DCP pellet (¤ 13 mm) prepared by compaction of the DCP powder at 1100 MPa was hydrolyzed with an alkaline solution at 70°C for 24 h, and an HA pellet covered with nanocrystals was obtained.…”

Section: Bcp Pellet Preparationmentioning

confidence: 99%

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Control of cellular activity of osteoblastic cells with microtopography of biphasic calcium phosphate scaffolds

Okada¹,

Oaki²,

Komotori³

et al. 2011

J. Ceram. Soc. Japan

Self Cite

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We studied the activity of osteoblastic cells on biphasic calcium phosphate (BCP) containing ca. 50 wt % hydroxyapatite and ca. 50 wt % ¢-tricalcium phosphate. The influence of the microtopography on the cellular activity was investigated by using three kinds of BCP scaffolds, which were composed of densely packed domains of 35 µm in size (dense-BCP), a porous framework of ³1 µm in width (micro-BCP), and aggregated particles of ³100 nm in diameter (nano-BCP). Basically, stable adhesion of the cells was achieved with defined focal adhesions on the BCP scaffolds regardless of their topography. The alkaline phosphatase (ALP) activity in the initial stage of the adhesion was more enhanced on micro-and nano-BCPs than it was on dense-BCPs due to the presence of a large number of calcium ions eluted from small grains in the micro-and nano-BCPs. However, the proliferation and the ALP activity were suppressed in the long-term culture on nano-BCP because of the limitation of the domain area for the formation of focal adhesions. Therefore, the cellular activity was successfully controlled with the surface microtopography of the scaffolds. The microstructured surface providing a sufficient domain area and essential ions would be suitable as a scaffold for osteoblastic cells.

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Small‐Molecule Chemistry Effect on the Functionalization of Polydimethylsiloxane with Hydroxyapatite

Öztatlı,

Erenay,

Karasu

et al. 2023

Adv Eng Mater

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Herein, polydimethylsiloxane (PDMS) surfaces are coated with hydroxyapatite (HA) using immobilization and biomineralization techniques with the aid of small molecules, namely, l‐aspartic acid (AA), l‐glutamic acid, and citric acid, in an effort to develop biointeractive surfaces for bone tissue‐related research. The efficacy of biomineralization and immobilization techniques and the impact of small molecules on HA deposition on PDMS surfaces are investigated by chemical and morphological analysis of surfaces, besides in vitro cell culture studies. Characteristic peaks of phosphate groups at wavelengths of 569 and 603 cm−1 in Fourier transform infrared spectroscopy analysis and HA's characteristic patterns identified at 26.1°, 31.7°, and 45.5° in X‐ray diffraction analysis demonstrate the successful deposition of HA particles on PDMS surfaces which are also observed on scanning electron microscopy micrographs. The interaction of human fetal osteoblast cells (hFOB 1.19) with HA‐deposited PDMS surfaces is evaluated with in vitro cell culture studies, which reveals increased cell metabolic activity and growth across all groups as compared to plain PDMS, with HA deposited on AA‐PDMS surfaces through biomineralization being the most stimulating group. Overall, small‐molecule effect on deposition of HA on PDMS substrates merits further investigation, particularly on substrates mimicking bone surface chemical and morphological features.

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Adhesion of osteoblast-like cells on nanostructured hydroxyapatite

Cited by 120 publications

References 29 publications

Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton’s jelly in vitro

Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton’s jelly in vitro

Control of cellular activity of osteoblastic cells with microtopography of biphasic calcium phosphate scaffolds

Small‐Molecule Chemistry Effect on the Functionalization of Polydimethylsiloxane with Hydroxyapatite

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