Adhesion of human platelets to collagen detected by 51Cr labelling and acoustic wave sensor

Muratsugu, Makoto; Romaschin, Alex; Thompson, Michael

doi:10.1016/s0003-2670(96)00576-4

Cited by 31 publications

(25 citation statements)

References 35 publications

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“…Going a step further, whole cells can be monitored when they are bound to the QCM surface. A number of surface-adherent cell types have been examined, including osteoblasts, human platelets, MDCK I and II cells, 3T3 cells, VERO cells, CHO and MKE epithelial cells, and microbial biofilms (23)(24)(25)(26)(27)(28)(29)(30). Notwithstanding considerable variability in reported results, these studies all serve to establish the basic principle, which is that adherent cells cause a reversible QCM frequency shift.…”

Section: Introductionmentioning

confidence: 99%

The Quartz Crystal Microbalance as a Continuous Monitoring Tool for the Study of Endothelial Cell Surface Attachment and Growth

et al. 2000

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The quartz crystal microbalance (QCM) was used to monitor endothelial cell (EC) adhesion on the gold surface of an oscillating quartz crystal contained in a QCM device. A number of parameters were investigated. First, we observed differential QCM O-ring toxicities for ECs. Second, appropriate conditions for cell culture and QCM cell environment were identified that can eliminate large-scale frequency oscillations in the measurements. These artifacts are not due to added cells but originate in the time-dependent evaporation of water. Having eliminated these artifacts, we then demonstrated that the measured steady-state crystal frequency shift, Delta f, and motional resistance shift, DeltaR, were determined by the number of firmly attached ECs requiring trypsinization from the crystal surface. Last, following steady-state attachment of ECs, the EC growth stimulation by fibroblast growth factor was monitored in a continuous fashion by measuring f and R values over a 72 h. period. We observed the Delta f values to increase in a way that reflected the increase in EC number bound to the QCM surface. Following addition of ECs to the QCM, the time-dependent increase in DeltaR can be interpreted in terms of increase by the ECs of the energy dissipation properties of the solution at the solution-gold surface interface. This effect is due to their rapid surface attachment and the elaboration of their cytoskeletal properties. These results indicate that the QCM technique can be used for the study of EC attachment and growth and suggest its potential for the real time study of per unit surface area cell mass distribution dynamics and viscoelastic properties and the cells' responses to stresses or perturbations brought about using biologically active molecules.

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Section: Introductionmentioning

confidence: 99%

The Quartz Crystal Microbalance as a Continuous Monitoring Tool for the Study of Endothelial Cell Surface Attachment and Growth

et al. 2000

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“…TSM devices with gold electrode surfaces modified with collagen responded substantially more to the specific adhesion of platelets than the interfaces modified with albumin [67]. Assuming the validity of Sauerbrey's concept, and considering the amount of platelets counted by radiolabelling, the expected frequency response should have been significantly larger than that actually measured in the study.…”

Section: Protein Adsorption Studiesmentioning

confidence: 70%

Biosensors Based on Acoustic Wave Devices

et al. 1999

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“…Infrequently, whole cells have been studied at the QCM surface (Gryte et al, 1993;Muratsugu et al, 1997;Wegener et al, 1998). Marx suggests the potential of this cellular biosensor for the real-time identification or screening of all classes of biologically active drugs or biological macromolecules that affect cellular attachment and cellular spreading, regardless of their molecular mechanism of action (Marx et al, 2003(Marx et al, , 2007.…”

Section: The Qcm In Cell Biologicalmentioning

confidence: 99%