Adhesion of hematopoietic progenitor cells to human mesenchymal stem cells as a model for cell−cell interaction

Improved lentiviral transduction of human mesenchymal stem cells for therapeutic intervention in pancreatic cancer

“…20 In addition, we cultured the MSC in medium for hematological cells (RPMI, 10% fetal calf serum (FCS) or Stemline II medium, thrombospondin (TPO 80 ng ml À1 ), stem cell factor (SCF, 50 ng ml À1 ), FL-3 ligand (50 ng ml À1 )) and did not find differentiation of the MSC to a CD34 þ phenotype. 22 In brief, the mononuclear cell fraction was isolated by Biocoll densitygradient centrifugation (d ¼ 1.077 g cm À3 ; Biochrom, Berlin, Germany). MSC were cultivated as described.…”

Exploring the Human Term Placenta as a Novel Source for Stem Cells and their Application in the Clinic

“…However, MSC have also been shown to be effective when sourced from other tissues including human placenta , umbilical cord (Bakhshi et al, 2008, Huang et al, 2007, Wang et al, 2004 and adipose tissue (Nakao et al, 2010). Recent papers have demonstrated that many of the specific cell-cell interactions between HSC and stromal cells are critical and may be essential for HSC regulation both in vivo (Steiner et al, 2009) and in vitro (Jing et al, 2010, Song et al, 2010, Wagner et al, 2008, Wagner et al, 2007, Wein et al, 2010. Indeed, a majority of studies have shown that cell-cell contact between HSC and MSC is essential for their ex vivo expansion.…”

MicroRNAs are shaping the hematopoietic landscape

“…Mesenchymal stromal cells (MSC) are precursors for osteoblasts, adipocytes and chondrocytes 59 and there is sound evidence that interaction of HSC and MSC is involved in maintenance and regulation of stem cell function. [60][61][62][63] MicroRNAs regulate proliferation and differentiation of MSC; 64 miR-125b, miR-146a and miR-196a affect osteogenic differentiation and cell proliferation. [65][66][67] Adipogenic differentiation is stimulated by miR-143 68 and miR-204 69 whereas miR-21 and miR-27 have been shown to down-regulate adipogenesis by directly targeting the adipogenic transcription factor PPARG.…”