Adhesion of Candida albicans to various dental implant surfaces and the influence of salivary pellicle proteins

Bürgers, Ralf; Hahnel, Sebastian; Reichert, Torsten E.; Rosentritt, Martin; Behr, Michaël; Gerlach, Till; Handel, Gerhard; Gosau, Martin

doi:10.1016/j.actbio.2009.11.003

Cited by 115 publications

(128 citation statements)

References 41 publications

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“…Further studies using appropriate models (e.g., a well-established in vivo biofilm-associated rat catheter model [51]) are required to confirm this synergy. Although the primary application of OSIP108 could be envisaged in systemic combination therapy, another potential application concerns its use as a coating of specific medical devices, such as dental implants, dentures, and voice prostheses, as C. albicans is the most prevalent fungus in the oral cavity (52) and is the most frequently isolated Candida spp. on voice prostheses in laryngectomized patients (53).…”

Section: Resultsmentioning

confidence: 99%

Plant-Derived Decapeptide OSIP108 Interferes with Candida albicans Biofilm Formation without Affecting Cell Viability

Delattin

Brucker

Craik

et al. 2014

Antimicrob Agents Chemother

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We previously identified a decapeptide from the model plant Arabidopsis thaliana, OSIP108, which is induced upon fungal pathogen infection. In this study, we demonstrated that OSIP108 interferes with biofilm formation of the fungal pathogen Candida albicans without affecting the viability or growth of C. albicans cells. OSIP108 displayed no cytotoxicity against various human cell lines. Furthermore, OSIP108 enhanced the activity of the antifungal agents amphotericin B and caspofungin in vitro and in vivo in a Caenorhabditis elegans-C. albicans biofilm infection model. These data point to the potential use of OSIP108 in combination therapy with conventional antifungal agents. In a first attempt to unravel its mode of action, we screened a library of 137 homozygous C. albicans mutants, affected in genes encoding cell wall proteins or transcription factors important for biofilm formation, for altered OSIP108 sensitivity. We identified 9 OSIP108-tolerant C. albicans mutants that were defective in either components important for cell wall integrity or the yeast-to-hypha transition. In line with these findings, we demonstrated that OSIP108 activates the C. albicans cell wall integrity pathway and that its antibiofilm activity can be blocked by compounds inhibiting the yeast-to-hypha transition. Furthermore, we found that OSIP108 is predominantly localized at the C. albicans cell surface. These data point to interference of OSIP108 with cell wall-related processes of C. albicans, resulting in impaired biofilm formation.

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Section: Resultsmentioning

confidence: 99%

Plant-Derived Decapeptide OSIP108 Interferes with Candida albicans Biofilm Formation without Affecting Cell Viability

Delattin

Brucker

Craik

et al. 2014

Antimicrob Agents Chemother

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show abstract

“…The shape, spatial and chemical structure, roughness, and surface energy have impact on the adhesion and proliferation of cells. However, the rough surface of the dental implants can promote the colonization by pathogens [27]. Therefore, the next challenge for the dental implantology is an indication of materials, which will limit the risk of colonization with pathogenic microorganisms.…”

Section: Discussionmentioning

confidence: 99%

In vitro biocompatibility of anodized titanium with deposited silver nanodendrites

et al. 2016

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Engineers searching new dental biomaterials try to modify the structure of the material in order to achieve the best performance as well as increased migration and proliferation of cells involved in the osseointegration of the implant. In this work we show in vitro test results of the Ti, which was anodically oxidized at high voltages with additionally deposited silver in the form of nanodendrites. The in vitro cytocompatibility of these materials was evaluated and compared with a conventional microcrystalline titanium. During the studies, established cell line of human gingival fibroblasts (HGF) and osteoblasts were cultured in the presence of tested materials, and its survival rate and proliferation activity were examined. Titanium samples modified with silver has a higher degree of biocompatibility in comparison with the unmodified reference material. Cells in contact with studied material showed a higher relative viability potential, stable level of proliferation activity, and lower rate of mortality. Biocompatibility tests carried out indicate that the anodically oxidized titanium at high voltages with additionally deposited nanosilver could be a possible candidate for dental implants and other medicinal applications.

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“…Among the microorganisms present in the oral cavity, lactic acid-producing bacterium Streptococcus mutans is of utmost importance as it is a well-known potential inducer of dental caries and has been shown to have an important role in periimplant diseases [20]. This pathogenic species is frequently associated with Candida albicans, a commensal fungal species often found in peri-implant lesions [21]. However, very little information is available on the formation of fungal or mixed biofilms on dental implants' surfaces.…”

Section: Introductionmentioning

confidence: 99%

Combined Influence of Fluoride and Biofilms on the Biotribocorrosion Behavior of Titanium Used for Dental Applications

et al. 2015

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Dental implant failures occur mainly due to biofilm infections and mastication micro-movements or load stresses that may induce wear degradation. Dental implants' microgaps are highly susceptible areas for biofilm formation and fluoride retention. Although fluoride is an antimicrobial agent widely used in caries prevention, its influence on biofilms under sliding wear remains unknown. The main aim of this work was to assess the effect of mixed biofilms and fluoride on the tribocorrosion behavior of titanium. Streptococcus mutans and Candida albicans mixed biofilms were cultured for 8 days on ground and polished commercially pure titanium. Biofilms' formation was influenced by topography: higher roughness surfaces presented higher levels of biomass. Reciprocating sliding tests were performed on both surfaces, with and without biofilms, immersed in artificial saliva in the absence or presence of fluoride (30 and 227 ppm F -). The low friction coefficient registered indicated the lubricating effect of biofilms on titanium surfaces. Fluoride was also found to have lubricating properties on titanium during sliding, probably due to the precipitation of calcium fluoride (or like) salts. The presence of fluoride influenced the biotribological behavior of titanium surfaces covered with biofilms, facilitating their structural disruption and detachment. Nevertheless, open circuit potential tests reduced the corrosion of titanium in the presence of fluoride (227 ppm F -) and biofilms. In conclusion, it is highlighted that fluoride interaction with biofilms can significantly influence the tribological properties of commercially pure titanium in the oral cavity.

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Adhesion of Candida albicans to various dental implant surfaces and the influence of salivary pellicle proteins

Cited by 115 publications

References 41 publications

Plant-Derived Decapeptide OSIP108 Interferes with Candida albicans Biofilm Formation without Affecting Cell Viability

Plant-Derived Decapeptide OSIP108 Interferes with Candida albicans Biofilm Formation without Affecting Cell Viability

In vitro biocompatibility of anodized titanium with deposited silver nanodendrites

Combined Influence of Fluoride and Biofilms on the Biotribocorrosion Behavior of Titanium Used for Dental Applications

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