Adhesion of Calcium Oxalate
Crystals to Madin-Darby Canine
Kidney Cells and Some Effects
of Glycosaminoglycans or
Cell Injuries

Ebisuno, Shoichi; Kohjimoto, Yasuo; Tamura, Miyako; Ohkawa, Tadashi

doi:10.1159/000475023

Cited by 29 publications

(32 citation statements)

References 12 publications

(18 reference statements)

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“…On the other hand, Lieskc et al [7] noted the engulfment of crystals into tubular epithelial cells in a transplanted kid ney of a patient with primary hyperoxaluria. They then confirmed this phenomenon experimentally using cal cium-containing crystals and tubular cells in culture [8,9], We have also observed that COM crystals adhered to the intact surface of Madin-Darby canine kidney (MDCK) cells, in a time-and concentration-dependent manner, and that the presence of glycosaminoglycans and cell inju ries reduced the attachment [10], In order to better understand the mechanisms in volved in crystal growth within the kidneys, it is necessary to identify the initial site and the detailed mechanisms of crystal deposition within the tubules. We present some histological evidence of the attachments of calcium oxa late crystals to three kinds of renal tubular cells, MDCK cells in culture, and rat and human kidney cells.…”

Section: Introductionmentioning

confidence: 54%

Histological Observations of the Adhesion and Endocytosis of Calcium Oxalate Crystals in MDCK Cells and in Rat and Human Kidney

Ebisuno¹,

Kohjimoto²,

Tamura³

et al. 1997

Urol Int

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Adhesion and/or endocytosis of calcium oxalate crystals to the three kinds of tubular cells (Madin-Darby canine kidney (MDCK) cells, rat and human kidney) were demonstrated morphologically to presume the initial formation of kidney stone. After removal of the nonadhesion crystals, the cells were subsequently recultured in the vertical position. At various times thereafter, the interactions between COM crystals and MDCK cells were evaluated morphologically by SEM. COM crystals adhered to the surface of MDCK cells immediately, and the crystals were then endocytosed. The microvilli of the cells appeared to play an important role in these processes. At later times, some complexes that consist of aggregated calcium oxalate crystals and cell debris were observed sporadically. Kidney tissues were obtained from male Sprague-Dawley rats which were injected with sodium oxalate intraperitoneally. Experimentally induced calcium oxalate crystals were evaluated histologically using polarized light microscopy. Some crystals in the cortical portion were attached to the tubular epithelium or internalized into the luminal membrane. Whereas in the papilla, the aggregated crystals were observed lying free from the degenerated tubular lumen along with the cell debris. Human kidney tissues were obtained from 38 patients with calcium oxalate nephrolithiasis who underwent nephrolithotomy or partial nephrectomy before the era of ESWL. The specimens were examined for calcium crystals within the tubular lumen, attached to the tubular walls or internalized into the tubular cells, by polarized light microscopy. Approximately 50% of the specimens observed crystals attached to the tubular cell epithelium and some of them were seen inside the tubular cells. In conclusion, crystal-cell interaction resulted in movement of crystals from the lumen into the cells by an action of microvilli from the results of MDCK cells. However, it was not clear from the results in rats or human kidney tissue that crystal adhesion and/or endocytosis might be vital in the crystal growth in the kidney.

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Section: Introductionmentioning

confidence: 54%

Histological Observations of the Adhesion and Endocytosis of Calcium Oxalate Crystals in MDCK Cells and in Rat and Human Kidney

Ebisuno¹,

Kohjimoto²,

Tamura³

et al. 1997

Urol Int

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“…In the previous reports indicated that GAGs play an important role in crystal-cell interactions [3,9,10]. Removal of GAGs from renal epithelial cell surfaces promotes crystal adhesion and the process is recovered by treatment with sulfated polysaccharides, such as heparin, HS and chondroitin sulfate [9,10].…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, several researchers have reported that heparin or HS inhibit calcium oxalate crystal aggregation, as well as crystal adhesion on the renal epithelial cells via the formation of a charge barrier [1][2][3]. Previously, we reported that HSPG (syndecan) mRNA significantly increased in the rat kidney during CaOx nephrolithiasis [4].…”

mentioning

confidence: 88%

“…Mardin-Darby canine kidney (MDCK) cells also have been used commonly. Recently, several investigators have reported that presence of GAGs, such as HS and chondroitin sulfate (ChS) prevent crystal attachment to the tubular epithelial surface [3,9,10]. Unfortunately, previous reports failed to confirm the exact role of cell surface HS, because the wild type MDCK cells do not express any type of cell surface heparan sulfate proteoglycan (HSPG, syndecan) [11].…”

mentioning

confidence: 99%

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Increased Expression of Heparan Sulfate Proteoglycan on the Cultured Renal Epithelial Cells during Oxalate Exposure

Matsuo

2008

Kurume Med. J.

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“…On the other hand, normal urothelium, especially that of the bladder, is covered by GAG layers, particularly important for mucosal resistance to bacterial adherence and which could act as a barrier against adhesion of crystals. Thus, disturbances in urinary GAG might play a critical role in calcium oxalate urolithiasis [16,[18][19][20].…”

Section: Discussionmentioning

confidence: 99%

Study of Calcium Oxalate Crystalluria on Renal and Vesical Urines in Stone Formers and Normal Subjects

Robert¹,

Boularan

Delbos³

et al. 1998

Urol Int

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Objective: The aim of this study is to compare vesical and renal calcium oxalate crystalluria in an attempt to correlate crystal formation with chemical composition and calcium oxalate saturation of renal urine. Material and Methods: Urine specimens were directly collected from the bladder and the kidney, of 11 stone formers and 11 control subjects under general anesthesia. The type of crystals present in urine as well as their size, number by cubic millimeter and state of aggregation were determined. In addition, calcium, magnesium, sodium, chloride, phosphate, citrate, oxalate, pyrophosphate and uric acid were measured in order to evaluate the calcium saturation status (EQUIL V program). Results: Calcium oxalate crystals were detected in 3 stone formers (27%) and 2 control subjects (18%) in vesical urine and in 4 stone formers (36%) and 3 control subjects (27%) in renal urine. Only 2 stone formers presented with simultaneous renal and vesical crystalluria. Subjects of the two groups with and without renal crystalluria were compared in terms of chemical composition and calcium oxalate saturation of renal urine. Crystalluric subjects (n = 7) had significantly higher uricosuria (p = 0.02), calciuria (p = 0.04), magnesiuria (p = 0.04) and calcium oxalate molar product (p = 0.05) than noncrystalluric (n = 15); calcium oxalate saturation was similar (p = 0.5). Conclusions: Beyond theorical considerations on lithogenesis, our observations and in particular the apparent discrepancy between renal and vesical crystalluria pose the problem of the clinical interest of the evaluation of calcium oxalate crystalluria based on freshly voided urine in the assessing the lithogenic risk or in the follow-up of patients who are particularly prone to stone recurrence.

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Adhesion of Calcium Oxalate Crystals to Madin-Darby Canine Kidney Cells and Some Effects of Glycosaminoglycans or Cell Injuries

Cited by 29 publications

References 12 publications

Histological Observations of the Adhesion and Endocytosis of Calcium Oxalate Crystals in MDCK Cells and in Rat and Human Kidney

Histological Observations of the Adhesion and Endocytosis of Calcium Oxalate Crystals in MDCK Cells and in Rat and Human Kidney

Increased Expression of Heparan Sulfate Proteoglycan on the Cultured Renal Epithelial Cells during Oxalate Exposure

Study of Calcium Oxalate Crystalluria on Renal and Vesical Urines in Stone Formers and Normal Subjects

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