Adhesion molecule expression and response to chemotactic agents of human monocyte-derived macrophages

Abstract

SUMMARYHuman monocyte-derived macrophages have been proposed as agents of anti-tumour immunotherapy. The aim of the present study was to investigate in vitro the properties of these cells likely to control their recruitment to the sites of inflammation and tumours. The expression of adhesion molecules involved in the binding of monocytes to endothelial cells was modified during monocyte-macrophage differentiation, with a significant increase in CD11c, CD14 and intercellular adhesion molecule-1 (ICAM-1). Monocyte-derived macrophages were sensitive to chemoattractants, in particular to the monocytespecific chemokine monocyte chemotactic protein-1 (MCP-1). They responded by an increased expression of adhesion molecules and were attracted by the cytokine in an under-agarose migration assay. The migration response, however, decreased after days 4-5 of monocyte differentiation into macrophage. In conclusion, human monocyte-derived macrophages show alterations of surface structures involved in the recognition of inflammatory endothelium. This may explain why the cells are poorly recruited to the sites of inflammation and tumours when introduced into the circulation.

TNF-α priming effect on polymorphonuclear leukocyte reactive oxygen species generation and adhesion molecule expression in hemodialyzed patients

“…The disproportionate increase in CD11b without an increase in CD18 forming the CD11b/CD18 heterodimer on the PMN surface does not provide any straightforward conclusions on the protein stoichiometry, as CD18 forms heterodimers also with CD11a and CD11c. Indeed, similar patterns of increased CD11b expression without accompanying CD18 increase was previously reported in flow cytometry analysis [1]. Despite the transient changes in PMN surface phenotype, reportedly related to complement activation in the course of reversible PMN sequestration during HD with cuprophane membranes, the up-regulated expressions of CD10, CD13, and CD11b were permanent, even though other CD receptor types returned to baseline following initial down-or up-regulation, in addition to those unaffected by the HD session [26].…”

Organization and Differential Expression of the Human Monocyte Chemoattractant Protein 1 Receptor Gene

“…In the case of both the type A and type B MCP-1 receptors, mRNA levels fell as the monocytes differentiated into macrophages. Taken together, these data are consistent with earlier studies in which radiolabeled MCP-1 failed to bind to macrophages (25)(26)(27) and suggest a possible mechanism for limiting the mobility of macrophages at sites of inflammation or injury.…”

Monocyte Subsets and Natural Killer Cells in Acute Pancreatitis

“…It is accompanied by an increase in CD11c and CD14 [38] . Moreover, monocytes and macrophages that were recovered from inflammatory sites, such as from the peritoneal fluid of patients with peritonitis, had a high expression of ICAM-1 [39] .…”