Adhesion-dependent rupturing of
            <i>Saccharomyces cerevisiae</i>
            on biological antimicrobial nanostructured surfaces

Nowlin, Kyle; Boseman, Adam; Covell, Alan D.; LaJeunesse, Dennis

doi:10.1098/rsif.2014.0999

Cited by 138 publications

(173 citation statements)

References 37 publications

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“…22,23 In follow-up studies, the nanopillars on the dragonfly wing were found to kill Gram-positive bacteria as well as yeast. 24,25 Similar nanopillars found on specially treated silicon wafers (black silicon) 26 had similar effects. According to these researchers, bacterial cells are killed on contact as they stretch over the pillars.…”

Section: Introductionsupporting

confidence: 55%

Nanopatterned polymer surfaces with bactericidal properties

Dickson¹,

Liang²,

Rodríguez³

et al. 2015

Biointerphases

233

240

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Bacteria that adhere to the surfaces of implanted medical devices can cause catastrophic infection. Since chemical modifications of materials' surfaces have poor long-term performance in preventing bacterial buildup, approaches using bactericidal physical surface topography have been investigated. The authors used Nanoimprint Lithography was used to fabricate a library of biomimetic nanopillars on the surfaces of poly(methyl methacrylate) (PMMA) films. After incubation of Escherichia coli (E. coli) on the structured PMMA surfaces, pillared surfaces were found to have lower densities of adherent cells compared to flat films (67%-91% of densities on flat films). Moreover, of the E. coli that did adhere a greater fraction of them were dead on pillared surfaces (16%-141% higher dead fraction than on flat films). Smaller more closely spaced nanopillars had better performance. The smallest most closely spaced nanopillars were found to reduce the bacterial load in contaminated aqueous suspensions by 50% over a 24-h period compared to flat controls. Through quantitative analysis of cell orientation data, it was determined that the minimum threshold for optimal nanopillar spacing is between 130 and 380 nm. Measurements of bacterial cell length indicate that nanopillars adversely affect E. coli morphology, eliciting a filamentous response. Taken together, this work shows that imprinted polymer nanostructures with precisely defined geometries can kill bacteria without any chemical modifications. These results effectively translate bactericidal nanopillar topographies to PMMA, an important polymer used for medical devices.

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Section: Introductionsupporting

confidence: 55%

Nanopatterned polymer surfaces with bactericidal properties

Dickson¹,

Liang²,

Rodríguez³

et al. 2015

Biointerphases

233

240

View full text Add to dashboard Cite

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“…In a mathematical model by Xue 27 explaining the mechanism of the bactericidal properties of the cicada wing, they suggest that when nanofeatures become sharper and the spacing between them is larger, the antibacterial properties of the surface are enhanced. Nowlin 28 also report that nanopillars with the smallest diameters were able to kill even the weakest adhering strain of Saccharomyces cerevisiae. This supports the findings from percentage kill differences between the nanocone features on surfaces A and B.…”

Section: B Topographical Effect On Bacterial Adhesion and Viabilitymentioning

confidence: 99%

Bactericidal activity of biomimetic diamond nanocone surfaces

Fisher

Yang²,

Yuen³

et al. 2016

Biointerphases

118

105

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The formation of biofilms on implant surfaces and the subsequent development of medical device-associated infections are difficult to resolve and can cause considerable morbidity to the patient. Over the past decade, there has been growing recognition that physical cues, such as surface topography, can regulate biological responses and possess bactericidal activity. In this study, diamond nanocone-patterned surfaces, representing biomimetic analogs of the naturally bactericidal cicada fly wing, were fabricated using microwave plasma chemical vapor deposition, followed by bias-assisted reactive ion etching. Two structurally distinct nanocone surfaces were produced, characterized, and the bactericidal ability examined. The sharp diamond nanocone features were found to have bactericidal capabilities with the surface possessing the more varying cone dimension, nonuniform array, and decreased density, showing enhanced bactericidal ability over the more uniform, highly dense nanocone surface. Future research will focus on using the fabrication process to tailor surface nanotopographies on clinically relevant materials that promote both effective killing of a broader range of microorganisms and the desired mammalian cell response. This study serves to introduce a technology that may launch a new and innovative direction in the design of biomaterials with capacity to reduce the risk of medical device-associated infections.

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“…20,22,[24][25][26]31,32,[34][35][36]38,[53][54][55][56][57] While the precise mechanism responsible for the antibacterial activity is still the subject of debate, 32,58 the consensus between the results obtained from both experimental [22][23][24][25][26]31,32,38,59,60 and theoretical 32,33 studies suggest that a torsional force is induced across the bacterial membrane upon surface adsorption.…”

Section: Concentrationsmentioning

confidence: 99%

Multi-directional electrodeposited gold nanospikes for antibacterial surface applications

et al. 2019

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The incorporation of high-aspect-ratio nanostructures across surfaces has been widely reported to impart antibacterial characteristics to a substratum. This occurs because the presence of such nanostructures can induce the mechanical rupture of attaching bacteria, causing cell death. As such, the development of highefficacy antibacterial nano-architectures fabricated on a variety of biologically relevant materials is critical to the wider acceptance of this technology. In this study, we report the antibacterial behavior of a series of substrata containing multi-directional electrodeposited gold (Au) nanospikes, as both a function of deposition time and precursor concentration. Firstly, the bactericidal efficacy of substrata containing Au nanospikes was assessed as a function of deposition time to elucidate the nanopattern that exhibited the greatest degree of biocidal activity. Here, it was established that multi-directional nanospikes with an average height of $302 nm AE 57 nm (formed after a deposition time of 540 s) exhibited the greatest level of biocidal activity, with $88% AE 8% of the bacterial cells being inactivated. The deposition time was then kept constant, while the concentration of the HAuCl 4 and Pb(CH 3 COO) 2 precursor materials (used for the formation of the Au nanospikes) was varied, resulting in differing nanospike architectures.Altering the Pb(CH 3 COO) 2 precursor concentration produced multi-directional nanostructures with a wider distribution of heights, which increased the average antibacterial efficacy against both Gramnegative Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus bacteria. Importantly, the in situ electrochemical fabrication method used in this work is robust and straightforward, and is able to produce highly reproducible antibacterial surfaces. The results of this research will assist in the wider utilization of mechano-responsive nano-architectures for antimicrobial surface technologies.

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Adhesion-dependent rupturing of Saccharomyces cerevisiae on biological antimicrobial nanostructured surfaces

Cited by 138 publications

References 37 publications

Nanopatterned polymer surfaces with bactericidal properties

Nanopatterned polymer surfaces with bactericidal properties

Bactericidal activity of biomimetic diamond nanocone surfaces

Multi-directional electrodeposited gold nanospikes for antibacterial surface applications

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