Nanopatterned polymer surfaces with bactericidal properties

Dickson, Mary Nora; Liang, Elena I.; Rodríguez, L. A.; Vollereaux, Nicolas; Yee, Albert F.

doi:10.1116/1.4922157

Cited by 227 publications

(261 citation statements)

References 35 publications

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“…This effect was reproduced with similar topographies on both black silicon [8] and PMMA [10]. To achieve antimicrobial nanopillar surfaces on Ti and Ti alloy implants, a fabrication technique is needed that can mimic the dimensions of such surface features found in nature, ideally on arbitrary shaped and porous materials.…”

Section: Introductionmentioning

confidence: 95%

“…This requires contact points between the bacterial cell wall and the surface that are much smaller than the bacteria and with sufficient spacing in between the contact points. Dickson et al [10] suggested that the spacing in between nanopillars should be between 130-380 nm. The nanospikes on our surfaces are more closely packed, but the random arrangement of the spikes means that bacteria can be exposed to sufficient local stress at selected points.…”

Section: E Coli In Vitro Studymentioning

confidence: 99%

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Bactericidal nanospike surfaces via thermal oxidation of Ti alloy substrates

Sjöström

Nobbs

2016

Materials Letters

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Highlights Thermal oxidation was used to grow bioinspired nanospikes on Ti alloy. Nanospike dimension and arrangement could be controlled. The method worked on arbitrary shaped Ti alloy surfaces. A 40 % reduction of E. Coli viability was achieved on Ti alloy nanospike surfaces. AbstractWith the aim to fabricate bio-inspired antibacterial nanotopography surfaces, nanospikes with varying dimensions were grown on Ti alloy surfaces using a thermal oxidation method. By controlling the acetone vapour concentration inside the tube furnace, the resulting oxide surface changed from nanocolumn shapes to nanospikes with approximately 20 nm diameters. The nanospikee growth was demonstrated to work on 3D Ti alloy bead surfaces, which means translation of the method to implant surfaces would be possible. Microbiology studies using Escherichia coli. showed that the nanospikes on the Ti alloy surfaces has potential to reduce bacterial viability. More dead bacteria were present on the nanospike surfaces compared to a smooth control and a 40 % reduction of viability was noted in bacterial suspensions incubated with a nanospike surface. It was shown that by annealing the Ti alloy surfaces prior to thermal oxidation, it is possible to grow vertically aligned nanospikes. This could be highly valuable when designing implant surfaces with antimicrobial properties.

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Section: Introductionmentioning

confidence: 95%

Section: E Coli In Vitro Studymentioning

confidence: 99%

Bactericidal nanospike surfaces via thermal oxidation of Ti alloy substrates

Sjöström

Nobbs

2016

Materials Letters

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“…However, research regarding the capacity for nanopatterned surfaces to attract bacterial attachment is contradictory. Dickson 10 found that lower cell densities were retained on PMMA pillared surfaces following 20 h culture compared to on flat surfaces. In contrast, other research findings have illustrated that the presence of nanofeatures can promote bacterial adhesion.…”

Section: B Topographical Effect On Bacterial Adhesion and Viabilitymentioning

confidence: 99%

“…Two of the most widely studied physical-based strategies for antimicrobial surfaces using micro- 8,9 or nanotopographies [10][11][12][13] are inhibition of microbial attachment (antibiofouling) or contact-killing. An example of an antibiofouling surface is the Sharklet micropattern surface, inspired by shark skin.…”

Section: Introductionmentioning

confidence: 99%

Bactericidal activity of biomimetic diamond nanocone surfaces

Fisher

Yang²,

Yuen³

et al. 2016

Biointerphases

114

105

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The formation of biofilms on implant surfaces and the subsequent development of medical device-associated infections are difficult to resolve and can cause considerable morbidity to the patient. Over the past decade, there has been growing recognition that physical cues, such as surface topography, can regulate biological responses and possess bactericidal activity. In this study, diamond nanocone-patterned surfaces, representing biomimetic analogs of the naturally bactericidal cicada fly wing, were fabricated using microwave plasma chemical vapor deposition, followed by bias-assisted reactive ion etching. Two structurally distinct nanocone surfaces were produced, characterized, and the bactericidal ability examined. The sharp diamond nanocone features were found to have bactericidal capabilities with the surface possessing the more varying cone dimension, nonuniform array, and decreased density, showing enhanced bactericidal ability over the more uniform, highly dense nanocone surface. Future research will focus on using the fabrication process to tailor surface nanotopographies on clinically relevant materials that promote both effective killing of a broader range of microorganisms and the desired mammalian cell response. This study serves to introduce a technology that may launch a new and innovative direction in the design of biomaterials with capacity to reduce the risk of medical device-associated infections.

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“…This effective nanospike height is consistent with that reported in a number of previous studies, 22,23,27,38,46-48 which suggests that a nanofeature height of $300 nm tends to display a greater level of biocidal activity towards both Gram-negative and Gram-positive bacteria than substrata possessing nanospikes with greater or less height. 22,23,27,38,[46][47][48] The reason for the effectiveness of this 'optimal' peak height in killing bacteria is still largely unknown. The inter-pillar spacing on the substrata used in this study was slightly larger than that of a number of previously reported effective antibacterial surfaces, which would initially suggest that nanofeature height plays an important role in the overall bactericidal behaviour of the substrates.…”

Section: 2526mentioning

confidence: 99%

Multi-directional electrodeposited gold nanospikes for antibacterial surface applications

et al. 2019

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The incorporation of high-aspect-ratio nanostructures across surfaces has been widely reported to impart antibacterial characteristics to a substratum. This occurs because the presence of such nanostructures can induce the mechanical rupture of attaching bacteria, causing cell death. As such, the development of highefficacy antibacterial nano-architectures fabricated on a variety of biologically relevant materials is critical to the wider acceptance of this technology. In this study, we report the antibacterial behavior of a series of substrata containing multi-directional electrodeposited gold (Au) nanospikes, as both a function of deposition time and precursor concentration. Firstly, the bactericidal efficacy of substrata containing Au nanospikes was assessed as a function of deposition time to elucidate the nanopattern that exhibited the greatest degree of biocidal activity. Here, it was established that multi-directional nanospikes with an average height of $302 nm AE 57 nm (formed after a deposition time of 540 s) exhibited the greatest level of biocidal activity, with $88% AE 8% of the bacterial cells being inactivated. The deposition time was then kept constant, while the concentration of the HAuCl 4 and Pb(CH 3 COO) 2 precursor materials (used for the formation of the Au nanospikes) was varied, resulting in differing nanospike architectures.Altering the Pb(CH 3 COO) 2 precursor concentration produced multi-directional nanostructures with a wider distribution of heights, which increased the average antibacterial efficacy against both Gramnegative Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus bacteria. Importantly, the in situ electrochemical fabrication method used in this work is robust and straightforward, and is able to produce highly reproducible antibacterial surfaces. The results of this research will assist in the wider utilization of mechano-responsive nano-architectures for antimicrobial surface technologies.

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Nanopatterned polymer surfaces with bactericidal properties

Cited by 227 publications

References 35 publications

Bactericidal nanospike surfaces via thermal oxidation of Ti alloy substrates

Bactericidal nanospike surfaces via thermal oxidation of Ti alloy substrates

Bactericidal activity of biomimetic diamond nanocone surfaces

Multi-directional electrodeposited gold nanospikes for antibacterial surface applications

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