Adhesion and biofilm formation by Staphylococcus aureus from food processing plants as affected by growth medium, surface type and incubation temperature

Jerônimo, Heloísa Maria Ângelo; Queiroga, Rita de Cássia Ramos do Egypto; Costa, Ana Caroliny Vieira da; Barbosa, Isabella de Medeiros; Conceição, Maria Lúcia da; Souza, Evandro Leite de

doi:10.1590/s1984-82502012000400018

Cited by 5 publications

(5 citation statements)

References 19 publications

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“…However, no difference was observed between 25 and 37 8C (P > 0.05). Similar results were obtained by Jeronimo et al, (2012), who found no difference between 28 and 37 8C on the adherence capability of S. aureus. We detected cell viability of the isolates in biofilm using the MTT assay (Fig.…”

Section: Resultssupporting

confidence: 88%

“…The biofilm forming capacity of S. aureus has been affected by several factors such as the nature of the surface, the growth medium, and other environmental conditions (Jeronimo et al, 2012;Zhao et al, 2017). In a study, most S. aureus strains produced more biofilm at 37 8C than at 25 8C, and showed better biofilm production with addition of glucose (Vazquez-Sanchez et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Biofilm formation, icaABCD genes and agr genotyping of Staphylococcus aureus from fish and ground beef

Özdemir

Arslan

Eken

2021

AAlim

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A total of 46 Staphylococcus aureus isolates from fish and ground beef were tested for the agr types, icaABCD genes, and biofilm formation at 12, 25 and 37 °C by the microtiter plate and the MTT assays. All isolates were positive for the icaABD genes, while 97.8% were positive for the icaC. All isolates produced biofilms at 37 and 25 °C, but 93.5% of them were also biofilm producers at 12 °C. There was no significant difference in biofilm formation between 25 and 37 °C using the crystal violet assay (P > 0.05). However, statistically significant differences were detected between 12 and 25 °C as well as 12 and 37 °C (P < 0.05). All isolates were significantly different in biofilm production by the MTT assay at all tested temperatures. Furthermore, a relationship between the presence of the icaABCD genes and biofilm formation was observed. The agr type I was the most prevalent (54.4%) among the isolates, followed by agr type II (41.3%) and agr type III (9.6%). In this study, the S. aureus isolates exhibited biofilm formation ability responsible for persistence of bacteria in foods, which may lead to food spoilage and human health problems.

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Section: Resultssupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Biofilm formation, icaABCD genes and agr genotyping of Staphylococcus aureus from fish and ground beef

Özdemir

Arslan

Eken

2021

AAlim

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“…The assayed strains revealed high capacities to adhere and form biofilms on polypropylene and stainless steel surfaces under different growth conditions, and the cells in biofilm matrixes were resistant to total removal when exposed to the sanitizers such as sodium hypochlorite and peracetic acid (Souza et al, 2014;Jerônimo et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Detection of exopolysaccharide production and biofilm-related genes in Staphylococcus spp. isolated from a poultry processing plant

Ferreira¹,

Tette²,

Mendonça³

et al. 2014

Food Sci. Technol (Campinas)

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Staphylococcus spp. can survive in biofilms for long periods of time, and they can be transferred from one point to another and cause environmental contamination in food processing. The aim of this study was to detect Staphylococcus strains isolated from a poultry processing plant by the presence of adhesion genes and the phenotypic production of exopolysaccharide. In the present study, the production of exopolysaccharide and the presence of adhesion genes in 65 strains of Staphylococcus spp. were evaluated. All strains of Staphylococcus spp. produced exopolysaccharide, as confirmed by formation of black and opaque colonies in Congo Red Agar. The variation of sucrose content was critical for the production of exopolysaccharide in Congo Red Agar since at low sucrose concentrations all strains presented a characteristic result, i.e., there was no exopolysaccharide production. The atl gene was found in all strains, and the icaA and icaD genes were found in 97% of them. The data obtained suggest that Staphylococcus spp. isolated from the poultry processing plant evaluated has a potential for biofilm formation. An efficient control of this microorganism in food processing environment is necessary as they may represent a potential risk to consumers.

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“…1). Different strains of S. aureus were characterized by intensive reversible attachment to surfaces which depends on the surface properties, available nutrients, mobility of the environment, exposure temperature and time [47][48][49]. In the presence of nutrients and under static conditions, S. aureus cells are fixed in large numbers on some surfaces [47][48][49].…”

Section: Attachment and Growth Of Bacteria On Apple Fruitsmentioning

confidence: 99%

“…Different strains of S. aureus were characterized by intensive reversible attachment to surfaces which depends on the surface properties, available nutrients, mobility of the environment, exposure temperature and time [47][48][49]. In the presence of nutrients and under static conditions, S. aureus cells are fixed in large numbers on some surfaces [47][48][49]. In this experiment, the weaker attachment of S. aureus and its easier removal from the apple surface by swabbing were probably caused by limited nutrients and the occasional shaking of the solution with the apples.…”

Section: Attachment and Growth Of Bacteria On Apple Fruitsmentioning

confidence: 99%

Attachment and survival of bacteria on apples with the creation of a kinetic mathematical model

et al. 2021

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The estimation of growth or inactivation of bacterial population in fruits during preservation and storage provides useful information for the improvement of the safety of fresh-cut fruits and vegetables. This paper addressed the attachment to the surface and the growth in the flesh of apple fruits of four bacterial cultures (Escherichia coli, Bacillus cereus, Staphylococcus aureus and Pseudomonas aeruginosa). The growth of the bacterial cultures in apple flesh was monitored at particular time intervals, and Gompertz parameters, i.e. maximum number of bacteria (P m ), the maximum growth rate of bacteria r p,m , and lag time t l , were used to determine the growth kinetics. After the immersion, the highest number of P. aeruginosa and the lowest number of B. cereus adhered to the apples. After washing and swabbing, E. coli was reduced from the surface of apples to the highest extent (by 3.34 log cfu g −1 ), while the number of B. cereus was reduced to the lowest extent (1.66 log cfu g −1 ). Fitted curves of the Gompertz model corresponded quite well to the measured values of the number of microorganisms with R 2 = 0.92-0.98. The values of the standard error (0.17-0.37) and extremely low p values of the Fischer test (p < 0.0001) indicated strict dependence between the model predicted and the maximum population density. The predicted values of the maximum number of microorganisms (P m ) correspond almost exactly to the actual values. A similar conclusion can be drawn for the maximum growth rate of microorganisms (r p,m ), with the measured value being slightly higher than predicted values.

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Adhesion and biofilm formation by Staphylococcus aureus from food processing plants as affected by growth medium, surface type and incubation temperature

Cited by 5 publications

References 19 publications

Biofilm formation, icaABCD genes and agr genotyping of Staphylococcus aureus from fish and ground beef

Biofilm formation, icaABCD genes and agr genotyping of Staphylococcus aureus from fish and ground beef

Detection of exopolysaccharide production and biofilm-related genes in Staphylococcus spp. isolated from a poultry processing plant

Attachment and survival of bacteria on apples with the creation of a kinetic mathematical model

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