Adhesin-dependent binding and uptake of Salmonella enterica serovar Typhimurium by dendritic cells

Guo, Aizhen; Lasaro, Melissa A.; Sirard, Jean‐Claude; Kraehenbuhl, Jean–Pierre; Schifferli, Dieter M.

doi:10.1099/mic.0.2006/000331-0

Cited by 37 publications

(51 citation statements)

References 57 publications

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“…Preparation of murine bone marrow-derived DCs and characterization of immature DCs were performed as described previously (Guo et al, 2007). Six-to 8-weekold C57BL/6N female mice were purchased from Zhongnan Hospital, Wuhan University, and temporarily housed in the animal facility at Huazhong Agricultural University.…”

Section: Methodsmentioning

confidence: 99%

Mycobacterium bovis and BCG induce different patterns of cytokine and chemokine production in dendritic cells and differentiation patterns in CD4+ T cells

Zhang

Luo

et al. 2013

Microbiology

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Mycobacterium tuberculosis can infect dendritic cells (DCs), but the molecular mechanism by which these cells contribute to tuberculosis pathogenesis is largely unclear. Using Mycobacterium bovis and the attenuated strain M. bovis BCG as model strains, we analysed cytokine and chemokine secretion in murine DCs infected with M. bovis and BCG at 6, 12 and 24 h post-infection. BCG enhanced production of MCP-1, RANTES, IL-12, TNF-a and IL-6 in DCs, while M. bovis promoted secretion of IL-1b, IL-10 and IL-23. Heat-killed BCG and M. bovis both stimulated cytokine production, but at significantly lower concentrations than corresponding live bacteria. Quantitative RT-PCR and Western blotting indicated that NF-kB regulates production of most cytokines and chemokines. After DCs were infected for 24 h, the culture was used to activate naïve CD4 + T cells. A combination of the supernatant and activated DCs infected with M. bovis gave high expression of foxp3 and IL-10, directing differentiation of naïve CD4 + T cells into regulatory T cells (CD4 + CD25 + Foxp3 + ) more effectively than BCG. Furthermore, M. bovis-infected DC cultures induced CD4 + T cells to express significantly higher levels of IL-17, a Th17-type cytokine, while BCG-infected DC cultures stimulated an apparently higher production of IFN-c, a Th1-type cytokine. In addition, the mycobacteria did not exert a direct effect on the differentiation of CD4 + T cells. These differential cytokine profiles in DCs and CD4 + T cells, and the resultant development of CD4 + T subsets, may be related to the pathogenesis of tuberculosis.

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Section: Methodsmentioning

confidence: 99%

Mycobacterium bovis and BCG induce different patterns of cytokine and chemokine production in dendritic cells and differentiation patterns in CD4+ T cells

Zhang

Luo

et al. 2013

Microbiology

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“…With the help of flagellar and chemotaxis machinery, the pathogen can cross the mucus barrier and gain access to the epithelial layer (6). Salmonella clings onto the epithelial cells with the help of flagella and fimbriae, inducing its uptake into the cells (4,(7)(8)(9)(10). The flagellin monomers bind to Toll-like receptor 5 (TLR5) on the epithelial cells (4), initiating signal transduction cascades that induce the expression of the proinflammatory genes via NF-B and mitogenactivated protein kinases (MAPKs), namely, p38, Jun amino-terminal kinase (JNK), extracellular signal-related kinase 1 (ERK1), and ERK2.…”

mentioning

confidence: 99%

Curcumin Reduces the Motility of Salmonella enterica Serovar Typhimurium by Binding to the Flagella, Thereby Leading to Flagellar Fragility and Shedding

et al. 2016

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One of the important virulence properties of the pathogen is its ability to travel to a favorable environment, cross the viscous mucus barrier (intestinal barrier for enteric pathogens), and reach the epithelia to initiate pathogenesis with the help of an appendage, like flagella. Nonetheless, flagella can act as an "Achilles heel," revealing the pathogen's presence to the host through the stimulation of innate and adaptive immune responses. We assessed whether curcumin, a dietary polyphenol, could alter the motility of Salmonella, a foodborne pathogen. It reduced the motility of Salmonella enterica serovar Typhimurium by shortening the length of the flagellar filament (from ϳ8 m to ϳ5 m) and decreasing its density (4 or 5 flagella/bacterium instead of 8 or 9 flagella/bacterium). Upon curcumin treatment, the percentage of flagellated bacteria declined from ϳ84% to 59%. However The flagellum is one of the important organelles for bacterial motility. It is made up of a hook-basal body, a motor complex, and a filament that is composed of around 20,000 flagellin monomers (1). The formation of the functional flagellar apparatus is highly coordinated and governed by environmental cues (2, 3). Flagella contribute to the virulence of the pathogen by aiding motility, adherence, and invasion of the host cells (4, 5). With the help of flagellar and chemotaxis machinery, the pathogen can cross the mucus barrier and gain access to the epithelial layer (6). Salmonella clings onto the epithelial cells with the help of flagella and fimbriae, inducing its uptake into the cells (4, 7-10). The flagellin monomers bind to Toll-like receptor 5 (TLR5) on the epithelial cells (4), initiating signal transduction cascades that induce the expression of the proinflammatory genes via NF-B and mitogenactivated protein kinases (MAPKs), namely, p38, Jun amino-terminal kinase (JNK), extracellular signal-related kinase 1 (ERK1), and ERK2. Flagellin protein also stimulates antibody production and maturation of dendritic cells (DCs), and it promotes antigen presentation by DCs in a MyD88-dependent manner (4). At the gut mucosal site, signaling through TLR5 is crucial, considering the downregulation of lipopolysaccharide (LPS)-mediated signaling via the TLR4 receptor (11). The proinflammatory responses mediated via TLR5 are essential in recruiting macrophages, dendritic cells, and neutrophils at the infection site. These cells sample the bacteria and disseminate them to systemic organs (4). A similar process occurs during infection with Salmonella enterica serovars Typhimurium (in mice) and Typhi (in humans), leading to systemic typhoid fever.Salmonellosis is endemic in Southeast Asian countries. In these countries, turmeric is widely used as a spice. Salmonella, being a

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“…It has been proposed that Salmonella from the intestinal lumen is absorbed via dendritic projections that pass between adjacent intestinal epithelial cells without disturbing the epithelial barrier integrity (Rescigno et al, 2001). This is through the utilization of fimbriae which selectively adheres Salmonella to dendritic projections, allowing Salmonella to invade DCs, thereby utilizing DCs as an additional entry point, as demonstrated in a recent study (Guo et al, 2007).…”

Section: Dendritic Cells (Dc)mentioning

confidence: 89%

“…A recent study has shown fimbriae, namely type 1 fimbrial adhesion FimH, mediates Salmonella uptake into murine DCs in a T3SS-independent fashion (Guo et al, 2007). It is thought that 13 fimbrial loci exist in Salmonella, which are thought to aid the internalization process through biofilm formation, attachment to host cells and colonization (Humphries et al, 2001).…”

Section: Fimbriae and Biofilmmentioning

confidence: 99%

Salmonella: Invasion, Evasion & Persistence

Chami¹,

Bao²

2012

Salmonella - Distribution, Adaptation, Control Measures and Molecular Technologies

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Adhesin-dependent binding and uptake of Salmonella enterica serovar Typhimurium by dendritic cells

Cited by 37 publications

References 57 publications

Mycobacterium bovis and BCG induce different patterns of cytokine and chemokine production in dendritic cells and differentiation patterns in CD4+ T cells

Mycobacterium bovis and BCG induce different patterns of cytokine and chemokine production in dendritic cells and differentiation patterns in CD4+ T cells

Curcumin Reduces the Motility of Salmonella enterica Serovar Typhimurium by Binding to the Flagella, Thereby Leading to Flagellar Fragility and Shedding

Salmonella: Invasion, Evasion & Persistence

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