Adherence pili of pathogenic strains of avian Escherichia coli

Vidotto, Marilda Carlos; Navarro, Helio R.; Gaziri, L. C. J.

doi:10.1016/s0378-1135(97)00124-7

Cited by 28 publications

(22 citation statements)

References 27 publications

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“…This study also confirms previous observations that type 1 fimbriae are frequently detected in APEC (7,20,29,40). A total of 93.7% E. coli isolates were found to hybridize with fim DNA probe, but only 26.5% exhibited mannose-sensitive hemagglutination pattern (MSHA).…”

Section: Discussionsupporting

confidence: 91%

“…Pourbakhsh et al (31) demonstrated the involvement of type 1 fimbriae in the colonization of the upper respiratory tract in experimentally inoculated chickens and suggested that P fimbriae may be involved in the colonization of internal organs and in the development of septicaemia. However, the major part of E.coli isolated from colibacillosis was negative for fimbriae P, and the mannose resistant adherence cannot always be attributed to P pili (33,40).…”

Section: Discussionmentioning

confidence: 99%

“…These virulence factors are very important for the host infection and to development of septicemia (10,24,29,(31)(32)(33)(34)39,40). Type 1 fimbriae have been involved with the initial stages of the upper respiratory colonization, whereas the P fimbriae are involved in colonization of the internal organs (31).…”

Section: Introductionmentioning

confidence: 99%

“…The role of these adhesins in pathogenesis of colibacillosis has not been elucidated, but poultry may act as a reservoir for human pathogenic E. coli (12,19,20,24,30,36,40).…”

Section: Introductionmentioning

confidence: 99%

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Some adhesins of avian pathogenic Escherichia coli (APEC) isolated from septicemic poultry in Brazil

Knöbl¹,

Gomes²,

Vieira³

et al. 2006

Braz. J. Microbiol.

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Three hundred and fifty strains of E. coli isolated from septicemic poultry from seven states of Brazil were examined for presence of nine adhesion-encoding genes, hemagglutination and adherence to chicken tracheal cells (in vitro). Analysis of the strains by colony hybridization tests demonstrated that 93.7% of the isolates were fim +, 17% pap+ and 5.7% were sfa+. The mannose sensitive fimbriae occur with similar frequency in APEC isolated from all Brazilians states, while significant differences among pap and sfa genes distributions were observed. The results showed that 0.85% and 0.28% of APEC were positive for genes that encoded enteroaggregative adhesins and EPEC adherence factor, respectively. None of APEC was positive for DA, afa, Bfp and Eae probes. The adherence to chicken tracheal cells showed 96% positive strains, while hemagglutination assays showed 26.5% of the isolates were mannose sensitive and 21.7% were mannose resistant.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…The role of these adhesins in pathogenesis of colibacillosis has not been elucidated, but poultry may act as a reservoir for human pathogenic E. coli (12,19,20,24,30,36,40).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Some adhesins of avian pathogenic Escherichia coli (APEC) isolated from septicemic poultry in Brazil

Knöbl¹,

Gomes²,

Vieira³

et al. 2006

Braz. J. Microbiol.

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“…Nas aves, a colibacilose inicia-se no epitélio traqueal, em contraste com a maioria das doenças causadas pela E. coli em humanos e outros mamíferos, afetados inicialmente no epitélio intestinal e urinário (ViDotto et al, 1997) e é responsável por condenações parciais ou totais das carcaças devido a vários processos inflamatórios, entre eles a aerossaculite (brasil, 1998).…”

Section: Introductionunclassified

PCR na detecção de gene Fel A de Escherichia Coli em frangos de corte condenados por aerossaculite pela Inspeção Sanitária Federal

et al. 2013

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A colibacilose é considerada uma das principais doenças da indústria avícola moderna, devido aos grandes prejuízos econômicos causados. A Escherichia coli contribui não só para a doença em si, levando à perda de peso das aves, bem como para o aumento da taxa condenação de carcaças durante o abate e processamento. A detecção de fatores de virulência de cepas de E. coli do patotipo APEC colabora para a caracterização de sua patogenicidade e as técnicas de PCR têm sido muito úteis na pesquisa de genes que os codificam. Este estudo objetivou diagnosticar E. coli pela detecção o gene Fel A por PCR e relacionar a positividade para este agente com o baixo peso em frangos de corte provenientes de lotes condenados por aerossaculite. Foram estudados 40 lotes de frangos de corte abatidos em um matadouro avícola sob Inspeção Sanitária Federal, localizado no Estado do Rio Grande do Sul. Foram colhidos aleatoriamente 3 frangos e obtidos "pools" de três traqueias em cada um deles para PCR. O DNA foi extraído pelo método de fenol-clorofórmio e amplificado com pares de "primers" específicos para gene Fel A de E. coli. Dos 40 lotes analisados pela PCR, 35% (14/40) foram positivos para o gene Fel A. A PCR foi eficaz para a detecção do gene Fel A em lotes de frangos de corte e houve relação entre a presença do gene Fel A, a queda de peso e aumento na taxa de aerossaculite.

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Virulence characteristics of Escherichia coli isolates obtained from broiler breeders with salpingitis

Monroy

Knöbl

Bottino

et al. 2005

Comparative Immunology, Microbiology and Infectious Diseases

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Adherence pili of pathogenic strains of avian Escherichia coli

Cited by 28 publications

References 27 publications

Some adhesins of avian pathogenic Escherichia coli (APEC) isolated from septicemic poultry in Brazil

Some adhesins of avian pathogenic Escherichia coli (APEC) isolated from septicemic poultry in Brazil

PCR na detecção de gene Fel A de Escherichia Coli em frangos de corte condenados por aerossaculite pela Inspeção Sanitária Federal

Virulence characteristics of Escherichia coli isolates obtained from broiler breeders with salpingitis

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