Adherence of Candida albicans to human buccal epithelial cells after growth on different carbon sources

Douglas, L

doi:10.1016/0378-1097(81)90144-0

Cited by 23 publications

(47 citation statements)

References 3 publications

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“…Previous investigations into factors affecting the adherence of C. albicans have shown that the level of adherence to epithelial tissue is in uenced by the carbon source in which the yeast is cultured [16,17]. C. albicans 10231, a commercially available Bakers' yeast product and four of the clinical S. cerevisiae isolates (1227, 1464, 128 and 436) were grown to the stationary phase in YEPD, YEPGal or YEPSuc and adherence assays were performed as described.…”

Section: Resultsmentioning

confidence: 99%

“…C. albicans 10231 and Bakers' yeast showed signi cant increases in adherence ability when cultured in galactose or sucrose while the adherence of clinical S. cerevisiae isolates to BECs showed no signi cant change when cultured in different carbon sources. The nature of the carbon source has been shown previously to have a profound effect on the adherence ability of C. albicans to BECs [16] and cultured cells [22] and has been linked to changes in the yeast cell surface, particularly in the production and secretion of mannoprotein, a putative adhesin associated with the brillar layer of the cell surface [23]. This work indicates that while the adherence of Bakers' yeast to BECs is in uenced by the nature of the carbon source used for growth this effect is not evident in clinical S. cerevisiae isolates.…”

Section: Discussionmentioning

confidence: 99%

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Adherence of clinical isolates of Saccharomyces cerevisiae to buccal epithelial cells

Murphy

Kavanagh

2001

Med Mycol

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A number of isolates of Saccharomyces cerevisiae have been associated with disease in immunocompromised individuals. Such isolates display a variety of characteristics that enable colonization and persistence in the host. The aim of the work presented here was to establish whether clinical isolates of S. cerevisiae were capable of adhering to epithelial tissue. Adherence to host tissue has been shown to be crucial to the virulence of the pathogenic yeast Candida albicans, and identi cation of this ability in S. cerevisiae might indicate a role for adherence in tissue colonization by this emerging pathogen. Clinical S. cerevisiae isolates were found to be capable of adhering to exfoliated buccal epithelial cells (BECs) but to a lesser degree than C. albicans. In contrast to the situation evident with C. albicans, the adherence of S. cerevisiae isolates to BECs was not in uenced by the carbon source in which the yeast was grown. Treatment of S. cerevisiae with trypsin or proteinase K resulted in a signi cant reduction in adherence ability while adherence was unaffected by treatment of cells with mannosidase, thus indicating a possible role for proteins rather than mannoproteins in the adherence of S. cerevisiae to BECs.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Adherence of clinical isolates of Saccharomyces cerevisiae to buccal epithelial cells

Murphy

Kavanagh

2001

Med Mycol

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“…N-Acetyl-Dglucosamine (GlcNAc) induction of hyphal formation (Mattia et al, 1982) was performed by resuspending cells that had been grown to stationary phase in supplemented Lee's medium, p H 4.5,37 "C, washed and starved in distilled water for 24 h, into 25 ml basal salt medium [0*5% (w/v) (NH,),SO,, 0.02 YO (w/v) MgSO,, 0.5 '/O (w/v) NaCl, 0.001 '/O biotin] with GlcNAc added to 4 mM, to a cell density of 2 x lo6 cells ml-l at 37 "C. Serum induction of hyphal formation (Gow & Gooday, 1982) was performed by resuspending cells that had been grown to stationary phase in supplemented Lee's medium, pH 4.5, 37 "C, and washed in Sullivan et al (1997) distilled water, into water containing 10 '/ o (v/v) newborn-calf serum (Sigma) to a cell density of 2 x lo6 cells ml-l at 37 "C. Adherence assays. Adherence assays were conducted on yeast cells grown for exactly 24 h in medium containing 0.67% (w/v) yeast nitrogen base without amino acids (Difco) and either 50 mM glucose or 500 mM galactose (Douglas et al, 1981). Yeast cells (1 x lo8 ml-l) and human buccal epithelial cells (BECs; 1 x lo5 ml-l) were incubated together for 45 min at 37 "C, then recovered on polycarbonate filters (pore size 12 pm; Costar) and Gram-stained.…”

Section: Methodsmentioning

confidence: 99%

“…It has not yet been established whether this increased adherence in relation to growth in glucose is a feature common to oral-adapted strains of Candida, or is peculiar to C. dubliniensis. Diabetes, antibiotic treatment and steroid therapy increase a patients level of salivary glucose -and all three conditions predispose a patient to candidosis (McCourtie & Douglas, 1981). It is possible that the increased adhesion of CD36 and other C .…”

Section: Ln Vivo Virulence Testsmentioning

confidence: 99%

Candida dubliniensis: phylogeny and putative virulence factors

et al. 1998

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Candida dubhiensis is a recently identified species which is implicated in oral candidosis in HIV-infected and AIDS patients. The species shares many phenotypic characteristics with, and is phylogenetically closely related to, Candida albicans. In this study the phylogenetic relationship between these two species was investigated and a comparison of putative virulence factors was performed. Four isolates of C. dubhiensis from different clinical sources were chosen for comparison with two reference C. albicans strains. First, the distinct phylogenetic position of C. dubhiensis was further established by the comparison of the sequence of its small rRNA subunit with representative Candida species. The C. dubhiensis isolates formed true unconstricted hyphae under most induction conditions tested but failed to produce true hyphae when induced using N-acetylglucosamine. Oral C. dubhiensis isolates were more adherent to human buccal epithelial cells than the reference C. albicans isolates when grown in glucose and equally adherent when grown in galactose. The C. dubhiensis isolates were sensitive to f luconazole, itraconazole, ketoconazole and amphotericin B. Homologues of seven tested C. albicans secretory aspartyl proteinase (SAP) genes were detected in C. dubhiensis by Southern analysis. In vivo virulence assays using a systemic mouse model suggest that C. dubhiensis is marginally less virulent than C. albicans. These data further confirm the distinct phenotypic and genotypic nature of C. dubhiensis and suggest that this species may be particularly adapted to colonization of the oral cavity.

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“…Fibrils mediating the adherence of C. albicans to surfaces have also been observed (27,31,41) and seem to be analogous to the fimbriae of bacteria (8,36) which play an important role in the establishment of adherence (38). The precise chemical nature of these morphological structures is still unknown (11) but they represent distinct adhering entities with a high ability to adhere to epithelial cells.…”

Section: Resultsmentioning

confidence: 99%

Experimental Model of Candida Albicans (Serotypes a and B) Adherence in Vitro

Pires

Corrêa

Gambale

et al. 2001

Braz. J. Microbiol.

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The adherence of Candida albicans is considered an important virulence factor since it determines the colonization of the yeast in various regions of the host, being the initial cause of the infection. In this work we studied the adherence of C. albicans (serotypes A and B) to HeLa and Vero cells after culture for 18 h and 30 h in chemically defined medium (YNB) plus carbohydrate sources such as glucose, sucrose, maltose, galactose and mannose (500 mM). Each assay was performed in triplicate. The best results were obtained with the medium containing glucose. The adherence was greatest in 18 h cultures at 37ºC and at 1 h contact time. The interaction of yeast cells with the host cells was studied by scanning microscopy.

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Adherence of Candida albicans to human buccal epithelial cells after growth on different carbon sources

Cited by 23 publications

References 3 publications

Adherence of clinical isolates of Saccharomyces cerevisiae to buccal epithelial cells

Adherence of clinical isolates of Saccharomyces cerevisiae to buccal epithelial cells

Candida dubliniensis: phylogeny and putative virulence factors

Experimental Model of Candida Albicans (Serotypes a and B) Adherence in Vitro

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