“…N-Acetyl-Dglucosamine (GlcNAc) induction of hyphal formation (Mattia et al, 1982) was performed by resuspending cells that had been grown to stationary phase in supplemented Lee's medium, p H 4.5,37 "C, washed and starved in distilled water for 24 h, into 25 ml basal salt medium [0*5% (w/v) (NH,),SO,, 0.02 YO (w/v) MgSO,, 0.5 '/O (w/v) NaCl, 0.001 '/O biotin] with GlcNAc added to 4 mM, to a cell density of 2 x lo6 cells ml-l at 37 "C. Serum induction of hyphal formation (Gow & Gooday, 1982) was performed by resuspending cells that had been grown to stationary phase in supplemented Lee's medium, pH 4.5, 37 "C, and washed in Sullivan et al (1997) distilled water, into water containing 10 '/ o (v/v) newborn-calf serum (Sigma) to a cell density of 2 x lo6 cells ml-l at 37 "C. Adherence assays. Adherence assays were conducted on yeast cells grown for exactly 24 h in medium containing 0.67% (w/v) yeast nitrogen base without amino acids (Difco) and either 50 mM glucose or 500 mM galactose (Douglas et al, 1981). Yeast cells (1 x lo8 ml-l) and human buccal epithelial cells (BECs; 1 x lo5 ml-l) were incubated together for 45 min at 37 "C, then recovered on polycarbonate filters (pore size 12 pm; Costar) and Gram-stained.…”