Adherence ability of Candida africana: a comparative study with Candida albicans and Candida dubliniensis

Romeo, Orazio; Leo, Filomena De; Criseo, Giuseppe

doi:10.1111/j.1439-0507.2009.01833.x

Cited by 23 publications

(16 citation statements)

References 25 publications

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“…africana among HIV‐infected patients in Cameroon. The isolation of the yeast in vaginal fluid is in accordance with what has been found previously, supporting the vaginal mucosa as a particular niche for C. africana . The patients’ CD4+ counts were higher enough to not consider the immunosuppression as being involved in the presence of the yeast.…”

Section: Discussionsupporting

confidence: 89%

“…The patients’ CD4+ counts were higher enough to not consider the immunosuppression as being involved in the presence of the yeast. In fact, it has already been shown that C. africana might colonise mucosae without a real implication in an infection . The patient with itching also presented Gardnerella vaginalis , a bacterium that colonises vaginal mucosa, thereby destroying Lactobacillus , providing a free space for the development of opportunistic pathogens such as Candida species .…”

Section: Discussionmentioning

confidence: 99%

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Investigation of minor species Candida africana, Candida stellatoidea and Candida dubliniensis in the Candida albicans complex among Yaoundé (Cameroon) HIV‐infected patients

Ngouana

Krasteva

Drakulovski

et al. 2014

Mycoses

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Minor species of the Candida albicans complex may cause overestimation of the epidemiology of C. albicans, and misidentifications could mask their implication in human pathology. Authors determined the occurrence of minor species of the C. albicans complex (C. africana, C. dubliniensis and C. stellatoidea) among Yaoundé HIV-infected patients, Cameroon. Stool, vaginal discharge, urine and oropharyngeal samples were analysed by mycological diagnosis. Isolates were identified by conventional methods and mass spectrometry (MS; carried out by the matrix-assisted laser desorption-ionisation time-of-flight MS protocol). Candida albicans isolates were thereafter submitted to the PCR amplification of the Hwp1 gene. The susceptibility of isolates to antifungal drugs was tested using the Clinical and Laboratory Standards Institute M27-A3 protocol. From 115 C. albicans obtained isolates, neither C. dubliniensis nor C. stellatoidea was observed; two strains of C. africana (422PV and 448PV) were identified by PCR electrophoretic profiles at 700 bp. These two C. africana strains were vaginal isolates. The isolate 448PV was resistant to ketoconazole at the minimal inhibitory concentration of 2 μg ml(-1), and showed reduced susceptibility to amphotericin B at 1 μg ml(-1). This first report on C. africana occurrence in Cameroon brings clues for the understanding of the global epidemiology of this yeast as well as that of minor species of the C. albicans complex.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Investigation of minor species Candida africana, Candida stellatoidea and Candida dubliniensis in the Candida albicans complex among Yaoundé (Cameroon) HIV‐infected patients

Ngouana

Krasteva

Drakulovski

et al. 2014

Mycoses

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“…The hwp1 gene in C. albicans is reported to be an important adhesin that crosslinks C. albicans to epithelial cells. The distinct hwp1 allele found in C. africana may be responsible for the low hyphal production, reduced adhesion and decreased virulence . Similarly, the rlm1 gene is required for cell wall integrity under stressed conditions such as the presence of the antifungal caspofungin.…”

Section: Discussionmentioning

confidence: 99%

Multilocus sequence typing of Candida africana from patients with vulvovaginal candidiasis in New Delhi, India

Sharma

Muralidhar

et al. 2014

Mycoses

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We investigated the prevalence of vulvovaginal candidiasis due to C. africana in an STD clinic in India and analysed the genetic relatedness of these C. africana isolates with those outside India. A total of 283 germ-tube-positive yeasts were identified by VITEK2. Molecular characterisation of all isolates was carried out by hwp1-gene-specific PCR. Of 283 germ-tube-positive yeast isolates, four were identified as C. africana using hwp1-gene-specific PCR. All hwp1 PCR positive C. africana were subjected to antifungal susceptibility testing, ITS and D1/D2 region sequencing and were typed by using MLST approach. Similar to C. africana isolates from the United Kingdom and unlike those from Africa, the Indian C. africana grew at 42°C. Sequencing of eight gene fragments in MLST identified all four strains to have different genotypes not reported previously. Furthermore, though the Indian C. africana isolates were susceptible to most of the 14 tested antifungal drugs, differences in susceptibility were observed among the four strains. Our results indicate genetic and phenotypic heterogeneity among C. africana from different geographical regions. Due to lack of data on epidemiology and genetic variability of this under-reported yeast, more studies using molecular methods are warranted.

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“…This amino sugar is metabolized by all typical C. albicans strains investigated (Tietz et al, 1995) and it is essential for various cellular processes in this species including white-opaque switching and yeast-to-hyphal transition (Huang et al, 2010; Konopka, 2012). In addition, C. africana also shows poor adhesion to human epithelial cells (Romeo et al, 2011), a notable low level of filamentation (Borman et al, 2013) and decreased virulence as recently demonstrated using both mammalian and insect infection models (Borman et al, 2013; Pagniez et al, 2015). All these characteristics could be the reflection of a peculiar genetic background that is worth to be investigated in detail.…”

Section: Introductionmentioning

confidence: 92%

Whole Genome-Based Amplified Fragment Length Polymorphism Analysis Reveals Genetic Diversity in Candida africana

et al. 2017

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This study aimed at investigating the genetic diversity of a panel of Candida africana strains recovered from vaginal samples in different countries. All fungal strains were heterozygous at the mating-type-like locus and belonged to the genotype A of Candida albicans. Moreover, all examined C. africana strains lack N-acetylglucosamine assimilation and sequence analysis of the HXK1 gene showed a distinctive polymorphism that impair the utilization of this amino sugar in this yeast. Multi-locus sequencing of seven housekeeping genes revealed a substantial genetic homogeneity among the strains, except for the CaMPIb, SYA1 and VPS13 loci which contributed significantly to the classification of our set of C. africana strains into six existing diploid sequence types. Amplified fragment length polymorphism fingerprint analysis yielded greater genotypic heterogeneity among the C. africana strains. Overall the data reported here show that in C. africana genetic diversity occurs and the existence of this intriguing group of C. albicans strains with specific phenotypes associated could be useful for future comparative studies in order to better understand the genetics and evolution of this important human pathogen.

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Adherence ability of Candida africana: a comparative study with Candida albicans and Candida dubliniensis

Cited by 23 publications

References 25 publications

Investigation of minor species Candida africana, Candida stellatoidea and Candida dubliniensis in the Candida albicans complex among Yaoundé (Cameroon) HIV‐infected patients

Investigation of minor species Candida africana, Candida stellatoidea and Candida dubliniensis in the Candida albicans complex among Yaoundé (Cameroon) HIV‐infected patients

Multilocus sequence typing of Candida africana from patients with vulvovaginal candidiasis in New Delhi, India

Whole Genome-Based Amplified Fragment Length Polymorphism Analysis Reveals Genetic Diversity in Candida africana

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