Aderência bacteriana e formação de biofilme aos fios de dermossustentação facial

Abstract

Flabbiness and expression wrinkles can be helped by undergoing a face lifting or by implanting subcutaneous tissues of polyurethane or polypropylene threads. Under certain conditions microorganisms can attach themselves to the threads and interact with these surfaces initiating cellular growth. The goal of the present study was to evaluate bacterial attachment to polyurethane and polypropylene threads by means of scanning electron microscopy and microbiological method. The threads were sectioned into segments of 1.0cm in length and inserted, one by one, into separated Falcon tubes (50 mL) containing Mueller Hinton broth (15 mL), with 200µl of the bacterial suspension (10 8 CFU/mL) prepared, and incubated for 1 hour and 30 minutes, at 4, 24, 48, 72 and 120 hour periods. After each incubation period, the coupons were rinsed three times and, inserted, one by one, into 5.0 mL of sterile physiological saline solution, sonicated at 40kHz for 8 minutes and vortexed for 10 seconds. This solution was diluted threefold (1/10 to 1/1000), from dilution the 1/1000 dilution an aliquot of 0.1 mL was plated onto Tryptic Soy agar (TSA). The plates were incubated at 37ºC from 18 to 24h. After the incubation periods, the viable bacteria were counted and the results noted in terms of colony forming units (CFU/ml). The coupons destined for scanning electron microscopy observations were fixed in glutaraldehyde, dehydrated in alcohol series, dried in vacuum centryfuge and metalized with gold. A quantitative evaluation was recorded of the growth of S. aureus on the surface of polyurethane, after 1h and 30 minutes of contact, the results shown 4.0±0.0 CFU/ml, S. epidermidis 4.07±0.1 CFU/ml and P. aeruginosa 5.08±0.14 CFU/ml. After 4-120h the number of viable cells of S. aureus on polyurethane surfaces were 5.49±0,04 CFU/ml, S. epidermidis 4.99±0.07 CFU/ml and P. aeruginosa 6.52±0.03 CFU/ml. A quantitative evaluation was recorded of the growth of S. aureus on the surface of polypropylene after 1h and 30 minutes of contact, the results shown 4.24±0.20 CFU/ml, with S. epidermidis 4.14±0.1 CFU/ml and P. aeruginosa 5.77±0.05 CFU/ml. After 4-120h the number of viable cells of S. aureus on polypropylene surfaces were 5.96±0.07 CFU/ml, S. epidermidis 4.96±0.07 CFU/ml and P. aeruginosa 6.63±0.05 CFU/ml. The number of viable cells of S. aureus, S. epidermidis, P. aeruginosa on polyurethane and polypropylene surfaces were significantly different (p<0.05). A biofilm was observed on both threads (polyurethane and polypropylene) as demonstrated by scanning electron microscopy.

Effect of polycaprolactone nanocapsules loaded with essential oils on biofilm formation by Staphylococcus aureus strains isolated from bovine mastitis cases

“…A previous test of biofilm formation was performed before treating different surfaces with the PCL nanocapsules. Maximum biofilm formation was observed after 120 h (5 days), and these results are concordant with those obtained by Leite (2008), where SEM images also showed maximum biofilm formation after 120 h. This stage was performed prior to the surfaces tests to avoid false positives, since a biofilm reading after the moment of maximum biofilm formation could demonstrate lower results due to the potential accumulation of toxic components of the colony itself, falsifying the results. In addition, this amount of time was possibly necessary for S. aureus to obtain a minor distance between material and colony bacteria.…”

Biofilme em marca-passo artificial: ficção ou realidade?

“…The subpectoral implantation technique proved to be a feasible alternative in the treatment of pacemaker generator pocket infection, showing 100% effectiveness, a shorter hospital stay and a lower cost. However, further studies and follow-up are necessary for a definitive conclusion [3][4][5][6][7][8][9][10][11][12] .…”

“…In biofilms, microcolonies are heterogeneous, consisting of microbial cells of one (monomicrobial) or more species (polymicrobial), functionally organized, where the organisms are protected from the action of macrophages and antibiotics. Moreover, the biofilm is roughly composed of 10 to 25% microorganisms, and 75 to 90% extracellular polymeric substances 11 .…”

“…One concern among scholars is the infection that the biofilm triggers, and in many cases of implants, device replacement is the alternative indicated 11 . Accordingly, it is worth noting that it is crucial to investigate the biofilm when there is persistence of infection 12 .…”

“…Several conducts have been described for the treatment of infection of pacemaker pocket. More conservative therapeutic approaches seem to produce higher rates of relapse or failure of treatment [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18] , and the best results relate to more aggressive approaches, with complete removal and implantation of new endocardial systems [3][4][5][6][7][8] , which is usually associated with high hospitalization rates and high costs 3 .…”