Adenylate Energy Charge during Batch Culture of Beneckea natriegens

Niven, Donald F.; Collins, P.; Knowles, Christopher J.

doi:10.1099/00221287-98-1-95

Cited by 47 publications

(23 citation statements)

References 55 publications

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“…Growth requires sea-salt-based medium. It grows in the mineral medium of Niven (1977), although it grows better with organic nitrogen than with ammonium. When tyrosine or peptone are present, eumelanin is produced during late phases of growth.…”

Section: Abbreviation : Cp Complex Polysaccharidesmentioning

confidence: 99%

Phylogenetic characterization of marine bacterium strain 2-40, a degrader of complex polysaccharides.

González¹,

Weiner²

2000

International Journal of Systematic and Evolutionary Microbiology

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The marine bacterium strain 2-40 was isolated from the salt marsh cord grass, Spartina alterniflora, in the Chesapeake Bay watershed, VA, USA. It is Gram-negative, requires sea salts and is a strict aerobe. It degrades numerous complex polysaccharides and synthesizes eumelanin. By 16S rDNA analysis, the isolate was shown to be a member of the γ-subclass of the Proteobacteria, related to Microbulbifer hydrolyticus and to a cellulolytic nitrogen-fixing bacterium.

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Section: Abbreviation : Cp Complex Polysaccharidesmentioning

confidence: 99%

Phylogenetic characterization of marine bacterium strain 2-40, a degrader of complex polysaccharides.

González¹,

Weiner²

2000

International Journal of Systematic and Evolutionary Microbiology

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“…Measurements of ATP level can be complemented with those of AMP and ADP, which allows the energy charge [(ATP + +ADP)/(ATP + ADP + AMP)] to be calculated (Atkinson, 1968 ; Bomsel & Pradet, 1968). This method has been used for several years as a biomass criterion in fields such as oceanography (Holm-Hansen, 1969), soil microbiology (MacLeold et al, 1969) and in bacteriology where it is useful in studying metabolic adaptation to different environmental conditions (see Niven et al, 1977;Knowles, 1977). To check the validity of this method for measuring the growth of Mollicutes, we have measured the ATP level and energy charge during the growth of batch cultures of Spiroplasma citri, together with other indicators of growth such as pH of the medium, number of colony-forming units (c.f.u.)…”

Section: Introductionmentioning

confidence: 99%

ATP and Energy Charge as Criteria of Growth and Metabolic Activity of Mollicutes: Application to Spiroplasma citri

Saglio

Daniels

Pradet³

1979

Journal of General Microbiology

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Intracellular ATP concentrations and energy charge were monitored during the growth of Spiroplasma citri and related to the number of colony-forming units, to the pH of the medium and to incorporation of radioactive precursors. Of three different methods used for extracting nucleotides -trichloroacetic acid, perchloric acid and boiling Tris/H,SO, buffer -the trichloroacetic acid treatment was the most effective. During the active growth phase the ATP concentration increased exponentially, in the same way as other growth parameters. However, as soon as the metabolic activity slowed down, there was a rapid drop of ATP concentration and of total adenylate content, which was followed much later by a parallel decrease in the number of colony-forming units. During active growth, energy charge remained at around 0.9 and decreased only slowly during the stationary phase. Incorporation of labelled thymidine or phenylalanine followed a different pattern during the late phase of the culture and gave less information concerning the viability of the organisms. I N T R O D U C T I O NThe methods available for measuring the growth of Mollicutes are limited and not always very suitable. In practice, only two standard methods give accurate results: (i) serial dilution followed by plating out, a sensitive, but time consuming method, and (ii) monitoring the incorporation of radioactive material into the culture (Saglio et al., 1973). The latter method is very simple and quick but does not discriminate between viable and non-viable organisms, which the former procedure does.The difficulties encountered when using other bacteriological techniques for measuring growth of Mollicutes have been summarized by Windsor & Boarer (1972) who described a rapid enumeration method based on the assessment of lactate dehydrogenase. The method is, of course, limited to organisms containing this enzyme and cannot detect less than lo7 organisms ml-I.For these reasons another criterion was sought to measure the growth of Mollicutes. namely ATP concentration. Study of ATP content as a growth criterion is facilitated by using the highly specific and very sensitive bioluminescent reaction of luciferase. Measurements of ATP level can be complemented with those of AMP and ADP, which allows the energy charge [(ATP + +ADP)/(ATP + ADP + AMP)] to be calculated

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“…The size of the adenine nucleotide pool and its proportional make-up, expressed as an energy charge of 0.8 to 0.9, are strictly controlled during growth of bacteria (Chapman, Fall & Atkinson, 1971 ;Niven, Collins & Knowles, 1977;Knowles, 1977).…”

Section: Introductionmentioning

confidence: 99%

“…Recent experiments on the adenine nucleotide content of growing Berzeckea natriegens have shown that, on starvation, the energy charge is maintained at the growth value of about 0.9 for several hours and there is little decrease in total nucleotide content (Niven et al, 1977). We have therefore examined the pathways of AMP catabolism by B. natriegens in order to determine how the adenine nucleotide content is regulated.…”

Section: Introductionmentioning

confidence: 99%

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Catabolism of Adenosine 5'-Monophosphate by Extracts of the Marine Bacterium Beneckea natriegens

Niven¹,

Collins

Knowles

1977

Journal of General Microbiology

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S U M M A R YAMP is catabolized by cell-free extracts of Benecked natriegens to inosine via adenosine by AMP nucleotidase and adenosine deaminase. In the presence of ATP, the AMP nucleotidase is inhibited and an AMP deaminase is activated, resulting in formation of IMP. When low concentrations of ATP are used, the IMP is converted, simultaneously with ATP consumption, to inosine by IMP nucleotidase, which is presumably ATP-sensitive. Since 5'-nucleotidases from various organisms are known to catabolize several ribonucleoside monophosphates, the AMP and IMP nucleotidase activities of B. natriegens may be due to the same enzyme. CTP, GTP and UTP inhibit AMP nucleotidase from B. natriegens without stimulating AMP deaminase, thus severely decreasing the rate of AMP breakdown.

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Adenylate Energy Charge during Batch Culture of Beneckea natriegens

Cited by 47 publications

References 55 publications

Phylogenetic characterization of marine bacterium strain 2-40, a degrader of complex polysaccharides.

Phylogenetic characterization of marine bacterium strain 2-40, a degrader of complex polysaccharides.

ATP and Energy Charge as Criteria of Growth and Metabolic Activity of Mollicutes: Application to Spiroplasma citri

Catabolism of Adenosine 5'-Monophosphate by Extracts of the Marine Bacterium Beneckea natriegens

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